[31] Functional analysis of ADP-ribosylation factor (ARF) guanine nucleotide exchange factors Gea1p and Gea2p in yeast

This chapter presents the functional analysis of adenosine diphosphate (ADP)-ribosylation factor (ARF) guanine nucleotide exchange factors Gea1p and Gea2p in yeast. The yeast Gealp protein has not been purified to homogeneity but useful information has been obtained as to its function from partial p...

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Veröffentlicht in:	Methods in Enzymology 2001, Vol.329, p.290-300
Hauptverfasser:	Peyroche, Anne, Jackson, Catherine L.
Format:	Artikel
Sprache:	eng
Schlagworte:	ADP-Ribosylation Factors - biosynthesis ADP-Ribosylation Factors - genetics ADP-Ribosylation Factors - isolation & purification ADP-Ribosylation Factors - metabolism Brefeldin A - pharmacology Chromatography, Affinity Culture Media - chemistry Electrophoresis, Polyacrylamide Gel Fungal Proteins - genetics Fungal Proteins - isolation & purification Fungal Proteins - metabolism Guanine Nucleotide Exchange Factors - genetics Guanine Nucleotide Exchange Factors - isolation & purification Guanine Nucleotide Exchange Factors - metabolism Guanosine 5'-O-(3-Thiotriphosphate) - metabolism Mutation Myristic Acid - metabolism Protein Binding Protein Transport - drug effects Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins
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