[51] Activation of dynamin GTPase activity by phosphoinositides and SH3 domain-containing proteins

This chapter describes procedures to isolate milligram quantities of dynamin-1 from bovine brain and to assay dynamin GTPase activity. The most commonly used methods to analyze GTP hydrolysis utilize radioactive nucleotide, labeled either in the α- or γ-phosphate. Production of guanosine 5'-dip...

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Veröffentlicht in:	Methods in Enzymology 2001, Vol.329, p.486-496
Hauptverfasser:	Barylko, Barbara, Binns, Derk D., Albanesi, Joseph P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adaptor Proteins, Signal Transducing Animals Brain - enzymology Buffers Cattle Dynamins Enzyme Activation - drug effects GRB2 Adaptor Protein GTP Phosphohydrolases - isolation & purification GTP Phosphohydrolases - metabolism Guanosine Triphosphate - metabolism Micelles Phosphatidylinositol 4,5-Diphosphate - pharmacology Phosphatidylinositols - pharmacology Proteins - chemistry Proteins - pharmacology src Homology Domains - physiology
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