Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene
The murine gap junction protein connexin43 (Cx43) is expressed in blood vessels, with vastly different contribution by endothelial and smooth muscle cells. We have used the Cre recombinase under control of TIE2 transcriptional elements to inactivate a floxed Cx43 gene specifically in endothelial cel...
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| Veröffentlicht in: | Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2001-01, Vol.29 (1), p.1-13 |
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| container_title | Genesis (New York, N.Y. : 2000) |
| container_volume | 29 |
| creator | Theis, Martin de Wit, Cor Schlaeger, Thorsten M. Eckardt, Dominik Krüger, Olaf Döring, Britta Risau, Werner Deutsch, Urban Pohl, Ulrich Willecke, Klaus |
| description | The murine gap junction protein connexin43 (Cx43) is expressed in blood vessels, with vastly different contribution by endothelial and smooth muscle cells. We have used the Cre recombinase under control of TIE2 transcriptional elements to inactivate a floxed Cx43 gene specifically in endothelial cells. Cre‐mediated deletion led to replacement of the Cx43 coding region by a lacZ reporter gene. This allowed us to monitor the extent of deletion and to visualize the endothelial expression pattern of Cx43. We found widespread endothelial expression of the Cx43 gene during embryonic development, which became restricted largely to capillaries and small vessels in all adult organs examined. Mice lacking Cx43 in endothelium did not exhibit altered blood pressure, in contrast to mice deficient in Cx40. Our results show that lacZ activation after deletion of the target gene allows us to determine the extent of cell type‐specific deletion after phenotypical investigation of the same animal. genesis 29:1–13, 2001. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/1526-968X(200101)29:1<1::AID-GENE1000>3.0.CO;2-0 |
| format | Article |
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We have used the Cre recombinase under control of TIE2 transcriptional elements to inactivate a floxed Cx43 gene specifically in endothelial cells. Cre‐mediated deletion led to replacement of the Cx43 coding region by a lacZ reporter gene. This allowed us to monitor the extent of deletion and to visualize the endothelial expression pattern of Cx43. We found widespread endothelial expression of the Cx43 gene during embryonic development, which became restricted largely to capillaries and small vessels in all adult organs examined. Mice lacking Cx43 in endothelium did not exhibit altered blood pressure, in contrast to mice deficient in Cx40. 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We have used the Cre recombinase under control of TIE2 transcriptional elements to inactivate a floxed Cx43 gene specifically in endothelial cells. Cre‐mediated deletion led to replacement of the Cx43 coding region by a lacZ reporter gene. This allowed us to monitor the extent of deletion and to visualize the endothelial expression pattern of Cx43. We found widespread endothelial expression of the Cx43 gene during embryonic development, which became restricted largely to capillaries and small vessels in all adult organs examined. Mice lacking Cx43 in endothelium did not exhibit altered blood pressure, in contrast to mice deficient in Cx40. Our results show that lacZ activation after deletion of the target gene allows us to determine the extent of cell type‐specific deletion after phenotypical investigation of the same animal. genesis 29:1–13, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>beta-Galactosidase - metabolism</subject><subject>blood pressure</subject><subject>Blood Pressure Determination</subject><subject>cell autonomous expression</subject><subject>Connexin 43 - genetics</subject><subject>Cre/loxP system</subject><subject>DNA - analysis</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>gap junctions</subject><subject>Gene Expression</subject><subject>Gene Targeting - methods</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Integrases - genetics</subject><subject>Integrases - metabolism</subject><subject>Lac Operon</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Stem Cells - physiology</subject><subject>Transcription, Genetic</subject><subject>vascular endothelium</subject><subject>Viral Proteins</subject><issn>1526-954X</issn><issn>1526-968X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFtvEzEQhVcIREvLX0D7hOBhg8de7yUgpGqbhkpVAwJE1JeR1zsOhr2E9UZt_j1eJU2fKlWW7JHnzDmaLwgyYBNgjH8AyZMoT7LlO84YMHjP8yl8gun07PI8ms-uZ17FPosJmxSLjzxiz4Ljw8jz-1rGy6PglXN_vFZmnL8MjgBAyFimx8Fy1lbd8Jtqu2kityZtjdVhT-taaWqoHcLOhL4f6q5t6c62sfBlZduVF61s14blNlShV9-MU10_UB-uqKXT4IVRtaPX-_ck-Hkx-1F8ia4W88vi7CrSccJZpCSDpExZpaDUAEYz4--cmUQkOdcG4lhDVgIjSirQqhRG-saozFRuMnESvN35rvvu34bcgI11mupatdRtHKZ-Zynz2Au_7oS675zryeC6t43qtwgMR9o44sIRHe5oI88R_EFPG-9po0CGxQI5Mm_5Zp-9KRuqHgz3eMVh6Vtb0_bJgY_kHf68b7TztW6gu4Ov6v9ikopU4q_rOco0v_lWXHzHVPwH_I-niw</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Theis, Martin</creator><creator>de Wit, Cor</creator><creator>Schlaeger, Thorsten M.</creator><creator>Eckardt, Dominik</creator><creator>Krüger, Olaf</creator><creator>Döring, Britta</creator><creator>Risau, Werner</creator><creator>Deutsch, Urban</creator><creator>Pohl, Ulrich</creator><creator>Willecke, Klaus</creator><general>John Wiley & Sons, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene</title><author>Theis, Martin ; 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals beta-Galactosidase - metabolism blood pressure Blood Pressure Determination cell autonomous expression Connexin 43 - genetics Cre/loxP system DNA - analysis Embryo, Mammalian - metabolism Endothelium, Vascular - cytology Endothelium, Vascular - metabolism gap junctions Gene Expression Gene Targeting - methods Genes, Reporter Humans Immunoenzyme Techniques Integrases - genetics Integrases - metabolism Lac Operon Mice Mice, Transgenic NG-Nitroarginine Methyl Ester - pharmacology Nitric Oxide Synthase - antagonists & inhibitors RNA, Messenger - biosynthesis Stem Cells - physiology Transcription, Genetic vascular endothelium Viral Proteins |
| title | Endothelium-specific replacement of the connexin43 coding region by a lacZ reporter gene |
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