[23] Expression and properties of Rab25 in polarized Madin-Darby canine kidney cells

This chapter discusses the expression and properties of Rab25 in polarized Madin-Darby canine kidney cells. In Madin-Darby canine kidney (MDCK) cells, Rab25 levels are very low, whereas endogenous Rablla levels are relatively high. Therefore, to study Rab25 in MDCK cells, cell lines are isolated sta...

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Veröffentlicht in:	Methods in Enzymology 2001, Vol.329, p.225-234
Hauptverfasser:	Goldenring, James R., Aron, Lorraine M., Lapierre, Lynne A., Navarre, Jennifer, Casanova, James E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Monoclonal - immunology Antibodies, Monoclonal - isolation & purification Antibody Specificity - immunology Cell Line Cell Polarity Dogs Enzyme-Linked Immunosorbent Assay - methods Fluorescent Antibody Technique Kidney - cytology Kidney - enzymology Microscopy, Fluorescence Molecular Sequence Data Peptide Fragments - immunology Peptide Fragments - metabolism rab GTP-Binding Proteins - analysis rab GTP-Binding Proteins - genetics rab GTP-Binding Proteins - immunology rab GTP-Binding Proteins - metabolism RNA, Messenger - analysis RNA, Messenger - genetics Transfection
Online-Zugang:	Volltext
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