Durability of sodium sulfate-treated polylysine-alginate microcapsules
Chelated hollow microcapsules are unstable under in vitro conditions because of their hygroscopic nature. Nongel inducing cations, such as Na+, stabilize the outer membrane of the alginate‐polylysine‐alginate microcapsules leading to more stable beads. We made different batches of empty capsules wit...
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| Veröffentlicht in: | Journal of biomedical materials research 2001-03, Vol.54 (3), p.396-399 |
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| creator | Darrabie, Marcus Freeman, Brandi Kaye Kendall Jr, William F. Hobbs, Hasan A. Opara, Emmanuel C. |
| description | Chelated hollow microcapsules are unstable under in vitro conditions because of their hygroscopic nature. Nongel inducing cations, such as Na+, stabilize the outer membrane of the alginate‐polylysine‐alginate microcapsules leading to more stable beads. We made different batches of empty capsules with a mean ± SEM diameter of 607 ± 11 microns, and found that within 1 week of incubating these capsules in normal saline at 37°C, they increased to 718 ± 10 microns (p < 0.05, n = 5). In initial experiments, we made different batches of capsules and divided them into two groups. One group was left untreated (control) whereas the other was treated with 6 mM Na2SO4 for 30 min, before incubation in saline at 37°C. Control capsules increased in weight and size, before beginning to melt in less than 1 week. In contrast, treated capsules rapidly lost weight and remained intact during 1 month of follow‐up. In perifusion experiments, we found no deleterious effect of sodium sulfate treatment on the function of islets enclosed in the capsules. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 396–399, 2001 |
| doi_str_mv | 10.1002/1097-4636(20010305)54:3<396::AID-JBM120>3.0.CO;2-B |
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Nongel inducing cations, such as Na+, stabilize the outer membrane of the alginate‐polylysine‐alginate microcapsules leading to more stable beads. We made different batches of empty capsules with a mean ± SEM diameter of 607 ± 11 microns, and found that within 1 week of incubating these capsules in normal saline at 37°C, they increased to 718 ± 10 microns (p < 0.05, n = 5). In initial experiments, we made different batches of capsules and divided them into two groups. One group was left untreated (control) whereas the other was treated with 6 mM Na2SO4 for 30 min, before incubation in saline at 37°C. Control capsules increased in weight and size, before beginning to melt in less than 1 week. In contrast, treated capsules rapidly lost weight and remained intact during 1 month of follow‐up. In perifusion experiments, we found no deleterious effect of sodium sulfate treatment on the function of islets enclosed in the capsules. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 396–399, 2001</description><identifier>ISSN: 0021-9304</identifier><identifier>EISSN: 1097-4636</identifier><identifier>DOI: 10.1002/1097-4636(20010305)54:3<396::AID-JBM120>3.0.CO;2-B</identifier><identifier>PMID: 11189046</identifier><identifier>CODEN: JBMRBG</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Absorbable Implants ; Alginates - chemistry ; Animals ; bioartificial pancreas ; Biological and medical sciences ; Capsules - chemistry ; Chelation ; diabetes ; Diabetes. Impaired glucose tolerance ; Drug Compounding ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Glucose - pharmacology ; Islets of Langerhans - drug effects ; Islets of Langerhans - metabolism ; Islets of Langerhans Transplantation - physiology ; Male ; Management. Various non-drug treatments. Langerhans islet grafts ; Medical sciences ; microcapsules ; Particle Size ; Polylysine - chemistry ; Rats ; Rats, Sprague-Dawley ; Saline water ; Scanning electron microscopy ; Sodium compounds ; Sulfates - chemistry ; transplantation ; Transplantation (surgical)</subject><ispartof>Journal of biomedical materials research, 2001-03, Vol.54 (3), p.396-399</ispartof><rights>Copyright © 2000 John Wiley & Sons, Inc.</rights><rights>2001 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3720-57fa184a6242974dfdc52268f35b477b0b32dae8a85bf076a0911e1503ca163b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1097-4636%2820010305%2954%3A3%3C396%3A%3AAID-JBM120%3E3.0.CO%3B2-B$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1097-4636%2820010305%2954%3A3%3C396%3A%3AAID-JBM120%3E3.0.CO%3B2-B$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=854708$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11189046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Darrabie, Marcus</creatorcontrib><creatorcontrib>Freeman, Brandi Kaye</creatorcontrib><creatorcontrib>Kendall Jr, William F.</creatorcontrib><creatorcontrib>Hobbs, Hasan A.</creatorcontrib><creatorcontrib>Opara, Emmanuel C.</creatorcontrib><title>Durability of sodium sulfate-treated polylysine-alginate microcapsules</title><title>Journal of biomedical materials research</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Chelated hollow microcapsules are unstable under in vitro conditions because of their hygroscopic nature. Nongel inducing cations, such as Na+, stabilize the outer membrane of the alginate‐polylysine‐alginate microcapsules leading to more stable beads. We made different batches of empty capsules with a mean ± SEM diameter of 607 ± 11 microns, and found that within 1 week of incubating these capsules in normal saline at 37°C, they increased to 718 ± 10 microns (p < 0.05, n = 5). In initial experiments, we made different batches of capsules and divided them into two groups. One group was left untreated (control) whereas the other was treated with 6 mM Na2SO4 for 30 min, before incubation in saline at 37°C. Control capsules increased in weight and size, before beginning to melt in less than 1 week. In contrast, treated capsules rapidly lost weight and remained intact during 1 month of follow‐up. In perifusion experiments, we found no deleterious effect of sodium sulfate treatment on the function of islets enclosed in the capsules. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 396–399, 2001</description><subject>Absorbable Implants</subject><subject>Alginates - chemistry</subject><subject>Animals</subject><subject>bioartificial pancreas</subject><subject>Biological and medical sciences</subject><subject>Capsules - chemistry</subject><subject>Chelation</subject><subject>diabetes</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Drug Compounding</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Glucose - pharmacology</subject><subject>Islets of Langerhans - drug effects</subject><subject>Islets of Langerhans - metabolism</subject><subject>Islets of Langerhans Transplantation - physiology</subject><subject>Male</subject><subject>Management. Various non-drug treatments. Langerhans islet grafts</subject><subject>Medical sciences</subject><subject>microcapsules</subject><subject>Particle Size</subject><subject>Polylysine - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Saline water</subject><subject>Scanning electron microscopy</subject><subject>Sodium compounds</subject><subject>Sulfates - chemistry</subject><subject>transplantation</subject><subject>Transplantation (surgical)</subject><issn>0021-9304</issn><issn>1097-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkV-L00AUxQdR3O7qV5CAIO5D6p3_SRVh27rrympRFMWXyySZyOikqZkEzbd3SmN9EcSnC4ffnLn3HEIWFOYUgD2hkOtUKK4eMwAKHOS5FAv-jOdqsbi4Xqevlq8pg-d8DvPV5ilLl7fI7PjoNplFE5rmHMQJOQ3hKwDkOad3yQmlNMtBqBm5XA-dKZx3_Zi0dRLayg1NEgZfm96mfWfjqJJd60c_Bre1qfFf3DaKSePKri3NLrI23CN3auODvT_NM_Lh8sX71cv0ZnN1vbq4SUuuGaRS14ZmwigmWK5FVVelZExlNZeF0LqAgrPK2MxksqhBKwM5pZZK4KWhihf8jDw6-O669vtgQ4-NC6X13mxtOwTUIHW8jP0TZFRIBkpG8N0BjNeE0Nkad51rTDciBdzXgPtEcZ8o_q4BpUCOsQbEWAMeaogK4GqDDJfR9MH0-1A0tvpjOeUegYcTYEJpfN2ZbenCkcuk0JDxY4o_nLfjfy32170mJfqmB18Xevvz6Gu6b6g01xI_vrnCT0v9dq3EZ-T8F5SCu1g</recordid><startdate>20010305</startdate><enddate>20010305</enddate><creator>Darrabie, Marcus</creator><creator>Freeman, Brandi Kaye</creator><creator>Kendall Jr, William F.</creator><creator>Hobbs, Hasan A.</creator><creator>Opara, Emmanuel C.</creator><general>John Wiley & Sons, Inc</general><general>John Wiley & Sons</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010305</creationdate><title>Durability of sodium sulfate-treated polylysine-alginate microcapsules</title><author>Darrabie, Marcus ; Freeman, Brandi Kaye ; Kendall Jr, William F. ; Hobbs, Hasan A. ; Opara, Emmanuel C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3720-57fa184a6242974dfdc52268f35b477b0b32dae8a85bf076a0911e1503ca163b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Absorbable Implants</topic><topic>Alginates - chemistry</topic><topic>Animals</topic><topic>bioartificial pancreas</topic><topic>Biological and medical sciences</topic><topic>Capsules - chemistry</topic><topic>Chelation</topic><topic>diabetes</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Drug Compounding</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Glucose - pharmacology</topic><topic>Islets of Langerhans - drug effects</topic><topic>Islets of Langerhans - metabolism</topic><topic>Islets of Langerhans Transplantation - physiology</topic><topic>Male</topic><topic>Management. Various non-drug treatments. Langerhans islet grafts</topic><topic>Medical sciences</topic><topic>microcapsules</topic><topic>Particle Size</topic><topic>Polylysine - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Saline water</topic><topic>Scanning electron microscopy</topic><topic>Sodium compounds</topic><topic>Sulfates - chemistry</topic><topic>transplantation</topic><topic>Transplantation (surgical)</topic><toplevel>online_resources</toplevel><creatorcontrib>Darrabie, Marcus</creatorcontrib><creatorcontrib>Freeman, Brandi Kaye</creatorcontrib><creatorcontrib>Kendall Jr, William F.</creatorcontrib><creatorcontrib>Hobbs, Hasan A.</creatorcontrib><creatorcontrib>Opara, Emmanuel C.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Darrabie, Marcus</au><au>Freeman, Brandi Kaye</au><au>Kendall Jr, William F.</au><au>Hobbs, Hasan A.</au><au>Opara, Emmanuel C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Durability of sodium sulfate-treated polylysine-alginate microcapsules</atitle><jtitle>Journal of biomedical materials research</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2001-03-05</date><risdate>2001</risdate><volume>54</volume><issue>3</issue><spage>396</spage><epage>399</epage><pages>396-399</pages><issn>0021-9304</issn><eissn>1097-4636</eissn><coden>JBMRBG</coden><abstract>Chelated hollow microcapsules are unstable under in vitro conditions because of their hygroscopic nature. Nongel inducing cations, such as Na+, stabilize the outer membrane of the alginate‐polylysine‐alginate microcapsules leading to more stable beads. We made different batches of empty capsules with a mean ± SEM diameter of 607 ± 11 microns, and found that within 1 week of incubating these capsules in normal saline at 37°C, they increased to 718 ± 10 microns (p < 0.05, n = 5). In initial experiments, we made different batches of capsules and divided them into two groups. One group was left untreated (control) whereas the other was treated with 6 mM Na2SO4 for 30 min, before incubation in saline at 37°C. Control capsules increased in weight and size, before beginning to melt in less than 1 week. In contrast, treated capsules rapidly lost weight and remained intact during 1 month of follow‐up. In perifusion experiments, we found no deleterious effect of sodium sulfate treatment on the function of islets enclosed in the capsules. © 2000 John Wiley & Sons, Inc. J Biomed Mater Res 54: 396–399, 2001</abstract><cop>New York</cop><pub>John Wiley & Sons, Inc</pub><pmid>11189046</pmid><doi>10.1002/1097-4636(20010305)54:3<396::AID-JBM120>3.0.CO;2-B</doi><tpages>4</tpages></addata></record> |
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| subjects | Absorbable Implants Alginates - chemistry Animals bioartificial pancreas Biological and medical sciences Capsules - chemistry Chelation diabetes Diabetes. Impaired glucose tolerance Drug Compounding Endocrine pancreas. Apud cells (diseases) Endocrinopathies Glucose - pharmacology Islets of Langerhans - drug effects Islets of Langerhans - metabolism Islets of Langerhans Transplantation - physiology Male Management. Various non-drug treatments. Langerhans islet grafts Medical sciences microcapsules Particle Size Polylysine - chemistry Rats Rats, Sprague-Dawley Saline water Scanning electron microscopy Sodium compounds Sulfates - chemistry transplantation Transplantation (surgical) |
| title | Durability of sodium sulfate-treated polylysine-alginate microcapsules |
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