Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery
Cell polarization is a crucial process during plant development, as well as in plant-microbe interactions, and is frequently associated with extensive cytoskeletal rearrangements. In interactions of plants with inappropriate fungal pathogens (so-called non-host interactions), the actin cytoskeleton...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2007-06, Vol.144 (2), p.1132-1143 |
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| creator | Miklis, Marco Consonni, Chiara Bhat, Riyaz A Lipka, Volker Schulze-Lefert, Paul Panstruga, Ralph |
| description | Cell polarization is a crucial process during plant development, as well as in plant-microbe interactions, and is frequently associated with extensive cytoskeletal rearrangements. In interactions of plants with inappropriate fungal pathogens (so-called non-host interactions), the actin cytoskeleton is thought to contribute to the establishment of effective barriers at the cell periphery against fungal ingress. Here, we impeded actin cytoskeleton function in various types of disease resistance using pharmacological inhibitors and genetic interference via ectopic expression of an actin-depolymerizing factor-encoding gene, ADF. We demonstrate that barley (Hordeum vulgare) epidermal cells require actin cytoskeleton function for basal defense to the appropriate powdery mildew pathogen Blumeria graminis f. sp. hordei and for mlo-mediated resistance at the cell wall, but not for several tested race-specific immune responses. Analysis of non-host resistance to two tested inappropriate powdery mildews, Erysiphe pisi and B. graminis f. sp. tritici, revealed the existence of actin-dependent and actin-independent resistance pathways acting at the cell periphery. These pathways act synergistically and appear to be under negative control by the plasma membrane-resident MLO protein. |
| doi_str_mv | 10.1104/pp.107.098897 |
| format | Article |
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In interactions of plants with inappropriate fungal pathogens (so-called non-host interactions), the actin cytoskeleton is thought to contribute to the establishment of effective barriers at the cell periphery against fungal ingress. Here, we impeded actin cytoskeleton function in various types of disease resistance using pharmacological inhibitors and genetic interference via ectopic expression of an actin-depolymerizing factor-encoding gene, ADF. We demonstrate that barley (Hordeum vulgare) epidermal cells require actin cytoskeleton function for basal defense to the appropriate powdery mildew pathogen Blumeria graminis f. sp. hordei and for mlo-mediated resistance at the cell wall, but not for several tested race-specific immune responses. Analysis of non-host resistance to two tested inappropriate powdery mildews, Erysiphe pisi and B. graminis f. sp. tritici, revealed the existence of actin-dependent and actin-independent resistance pathways acting at the cell periphery. These pathways act synergistically and appear to be under negative control by the plasma membrane-resident MLO protein.</description><identifier>ISSN: 0032-0889</identifier><identifier>ISSN: 1532-2548</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.107.098897</identifier><identifier>PMID: 17449647</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>Actin Depolymerizing Factors - metabolism ; Actins ; Actins - metabolism ; Bacterial Proteins - metabolism ; Barley ; Biological and medical sciences ; Blumeria graminis ; Cell kinetics ; Cell physiology ; Cytoskeleton - metabolism ; Epidermal cells ; Erysiphe pisi ; Fundamental and applied biological sciences. Psychology ; Genotype ; Genotypes ; Glucans - metabolism ; Hordeum - genetics ; Hordeum - metabolism ; Hordeum - microbiology ; Hordeum vulgare ; Microfilaments ; Mildews ; Pathogens ; Pharmacology ; Phosphorylation ; Plant cells ; Plant Diseases - microbiology ; Plant Epidermis - metabolism ; Plant physiology and development ; Plant Proteins - metabolism ; Plants ; Plants Interacting with Other Organisms</subject><ispartof>Plant physiology (Bethesda), 2007-06, Vol.144 (2), p.1132-1143</ispartof><rights>Copyright 2007 American Society of Plant Biologists</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-a8421198ea80007d47ab1895c8c14b4aa3b0c55fc9fc5e5fd794aa69a53b5e4d3</citedby><cites>FETCH-LOGICAL-c540t-a8421198ea80007d47ab1895c8c14b4aa3b0c55fc9fc5e5fd794aa69a53b5e4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40065557$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40065557$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18846736$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17449647$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miklis, Marco</creatorcontrib><creatorcontrib>Consonni, Chiara</creatorcontrib><creatorcontrib>Bhat, Riyaz A</creatorcontrib><creatorcontrib>Lipka, Volker</creatorcontrib><creatorcontrib>Schulze-Lefert, Paul</creatorcontrib><creatorcontrib>Panstruga, Ralph</creatorcontrib><title>Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Cell polarization is a crucial process during plant development, as well as in plant-microbe interactions, and is frequently associated with extensive cytoskeletal rearrangements. In interactions of plants with inappropriate fungal pathogens (so-called non-host interactions), the actin cytoskeleton is thought to contribute to the establishment of effective barriers at the cell periphery against fungal ingress. Here, we impeded actin cytoskeleton function in various types of disease resistance using pharmacological inhibitors and genetic interference via ectopic expression of an actin-depolymerizing factor-encoding gene, ADF. We demonstrate that barley (Hordeum vulgare) epidermal cells require actin cytoskeleton function for basal defense to the appropriate powdery mildew pathogen Blumeria graminis f. sp. hordei and for mlo-mediated resistance at the cell wall, but not for several tested race-specific immune responses. Analysis of non-host resistance to two tested inappropriate powdery mildews, Erysiphe pisi and B. graminis f. sp. tritici, revealed the existence of actin-dependent and actin-independent resistance pathways acting at the cell periphery. These pathways act synergistically and appear to be under negative control by the plasma membrane-resident MLO protein.</description><subject>Actin Depolymerizing Factors - metabolism</subject><subject>Actins</subject><subject>Actins - metabolism</subject><subject>Bacterial Proteins - metabolism</subject><subject>Barley</subject><subject>Biological and medical sciences</subject><subject>Blumeria graminis</subject><subject>Cell kinetics</subject><subject>Cell physiology</subject><subject>Cytoskeleton - metabolism</subject><subject>Epidermal cells</subject><subject>Erysiphe pisi</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Glucans - metabolism</subject><subject>Hordeum - genetics</subject><subject>Hordeum - metabolism</subject><subject>Hordeum - microbiology</subject><subject>Hordeum vulgare</subject><subject>Microfilaments</subject><subject>Mildews</subject><subject>Pathogens</subject><subject>Pharmacology</subject><subject>Phosphorylation</subject><subject>Plant cells</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Epidermis - metabolism</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>Plants Interacting with Other Organisms</subject><issn>0032-0889</issn><issn>1532-2548</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EokvLkSPgC9yytWM7do7LFkqlrVoJeo4mzqSbKuuktiO0_x5XCfTY04ze-_Q0mkfIB87WnDN5Po5rzvSalcaU-hVZcSXyLFfSvCYrxtLOknFC3oXwwBjjgsu35IRrKctC6hXpv4Hv8Uivdzf0emimHiIGurGxc9kFjugadJGCaxbtKgn_1I2LXTu5e-jpBbboAtJbiPs_cAwUIo17pFvse3qLvhv36I9n5E0LfcD3yzwldz--_97-zHY3l1fbzS6zSrKYgZE556VBMOlk3UgNNTelssZyWUsAUTOrVGvL1ipUbaPLJBYlKFErlI04JV_n3NEPjxOGWB26YNMp4HCYQqWZ0oUU5kUwHVFIlYsEZjNo_RCCx7YafXcAf6w4q556qMYxrbqae0j8pyV4qg_YPNPL4xPwZQEgWOhbD8524ZkzRhZaFIn7OHMPIQ7-vy8ZK5RSTzmfZ7-FoYJ7nzLufuWp5_Q4w7UW4i8nCKMN</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Miklis, Marco</creator><creator>Consonni, Chiara</creator><creator>Bhat, Riyaz A</creator><creator>Lipka, Volker</creator><creator>Schulze-Lefert, Paul</creator><creator>Panstruga, Ralph</creator><general>American Society of Plant Biologists</general><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070601</creationdate><title>Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery</title><author>Miklis, Marco ; Consonni, Chiara ; Bhat, Riyaz A ; Lipka, Volker ; Schulze-Lefert, Paul ; Panstruga, Ralph</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-a8421198ea80007d47ab1895c8c14b4aa3b0c55fc9fc5e5fd794aa69a53b5e4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Actin Depolymerizing Factors - metabolism</topic><topic>Actins</topic><topic>Actins - metabolism</topic><topic>Bacterial Proteins - metabolism</topic><topic>Barley</topic><topic>Biological and medical sciences</topic><topic>Blumeria graminis</topic><topic>Cell kinetics</topic><topic>Cell physiology</topic><topic>Cytoskeleton - metabolism</topic><topic>Epidermal cells</topic><topic>Erysiphe pisi</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Glucans - metabolism</topic><topic>Hordeum - genetics</topic><topic>Hordeum - metabolism</topic><topic>Hordeum - microbiology</topic><topic>Hordeum vulgare</topic><topic>Microfilaments</topic><topic>Mildews</topic><topic>Pathogens</topic><topic>Pharmacology</topic><topic>Phosphorylation</topic><topic>Plant cells</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Epidermis - metabolism</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - metabolism</topic><topic>Plants</topic><topic>Plants Interacting with Other Organisms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miklis, Marco</creatorcontrib><creatorcontrib>Consonni, Chiara</creatorcontrib><creatorcontrib>Bhat, Riyaz A</creatorcontrib><creatorcontrib>Lipka, Volker</creatorcontrib><creatorcontrib>Schulze-Lefert, Paul</creatorcontrib><creatorcontrib>Panstruga, Ralph</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miklis, Marco</au><au>Consonni, Chiara</au><au>Bhat, Riyaz A</au><au>Lipka, Volker</au><au>Schulze-Lefert, Paul</au><au>Panstruga, Ralph</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>144</volume><issue>2</issue><spage>1132</spage><epage>1143</epage><pages>1132-1143</pages><issn>0032-0889</issn><issn>1532-2548</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Cell polarization is a crucial process during plant development, as well as in plant-microbe interactions, and is frequently associated with extensive cytoskeletal rearrangements. In interactions of plants with inappropriate fungal pathogens (so-called non-host interactions), the actin cytoskeleton is thought to contribute to the establishment of effective barriers at the cell periphery against fungal ingress. Here, we impeded actin cytoskeleton function in various types of disease resistance using pharmacological inhibitors and genetic interference via ectopic expression of an actin-depolymerizing factor-encoding gene, ADF. We demonstrate that barley (Hordeum vulgare) epidermal cells require actin cytoskeleton function for basal defense to the appropriate powdery mildew pathogen Blumeria graminis f. sp. hordei and for mlo-mediated resistance at the cell wall, but not for several tested race-specific immune responses. Analysis of non-host resistance to two tested inappropriate powdery mildews, Erysiphe pisi and B. graminis f. sp. tritici, revealed the existence of actin-dependent and actin-independent resistance pathways acting at the cell periphery. These pathways act synergistically and appear to be under negative control by the plasma membrane-resident MLO protein.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>17449647</pmid><doi>10.1104/pp.107.098897</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Depolymerizing Factors - metabolism Actins Actins - metabolism Bacterial Proteins - metabolism Barley Biological and medical sciences Blumeria graminis Cell kinetics Cell physiology Cytoskeleton - metabolism Epidermal cells Erysiphe pisi Fundamental and applied biological sciences. Psychology Genotype Genotypes Glucans - metabolism Hordeum - genetics Hordeum - metabolism Hordeum - microbiology Hordeum vulgare Microfilaments Mildews Pathogens Pharmacology Phosphorylation Plant cells Plant Diseases - microbiology Plant Epidermis - metabolism Plant physiology and development Plant Proteins - metabolism Plants Plants Interacting with Other Organisms |
| title | Barley MLO Modulates Actin-Dependent and Actin-Independent Antifungal Defense Pathways at the Cell Periphery |
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