Structural and phylogenetic analysis of the γ-actin encoding gene from the penicillin-producing fungus Penicillium chrysogenum
The nucleotide sequence of a 2994-bp genomic fragment, including the gamma-actin encoding gene from Penicillium chrysogenum, has been determined, showing an open reading frame (ORF) of 1756 bp interrupted by five introns with fungal consensus splice-site junctions. The 5' untranslated region co...
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| Veröffentlicht in: | Current microbiology 2001-02, Vol.42 (2), p.117-121 |
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| creator | DIEZ, Bruno MARCOS, Ana Teresa RODRIGUEZ, Marta DE LA FUENTE, Juan Luis BARREDO, José Luis |
| description | The nucleotide sequence of a 2994-bp genomic fragment, including the gamma-actin encoding gene from Penicillium chrysogenum, has been determined, showing an open reading frame (ORF) of 1756 bp interrupted by five introns with fungal consensus splice-site junctions. The 5' untranslated region contains a consensus TATA box, five CAAT motifs, and two large pyrimidine stretches. The predicted protein (375 amino acids) revealed high identity to gamma-actins from fungi (>90%), and gene phylogenies support the grouping of P. chrysogenum actin close to those from the majority of the filamentous fungi. The actA gene is present as a single copy in the genome of P. chrysogenum, and its expression is constitutive during penicillin fermentation, showing a single 1.4-kb transcript. |
| doi_str_mv | 10.1007/s002840010189 |
| format | Article |
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The 5' untranslated region contains a consensus TATA box, five CAAT motifs, and two large pyrimidine stretches. The predicted protein (375 amino acids) revealed high identity to gamma-actins from fungi (>90%), and gene phylogenies support the grouping of P. chrysogenum actin close to those from the majority of the filamentous fungi. The actA gene is present as a single copy in the genome of P. chrysogenum, and its expression is constitutive during penicillin fermentation, showing a single 1.4-kb transcript.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s002840010189</identifier><identifier>PMID: 11136133</identifier><identifier>CODEN: CUMIDD</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>5' Untranslated Regions ; Actins - genetics ; Bacteriology ; Biological and medical sciences ; Cloning, Molecular ; Fundamental and applied biological sciences. 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The 5' untranslated region contains a consensus TATA box, five CAAT motifs, and two large pyrimidine stretches. The predicted protein (375 amino acids) revealed high identity to gamma-actins from fungi (>90%), and gene phylogenies support the grouping of P. chrysogenum actin close to those from the majority of the filamentous fungi. The actA gene is present as a single copy in the genome of P. chrysogenum, and its expression is constitutive during penicillin fermentation, showing a single 1.4-kb transcript.</description><subject>5' Untranslated Regions</subject><subject>Actins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>g-Actin</subject><subject>Gene Dosage</subject><subject>Genes, Fungal</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Penicillium chrysogenum</subject><subject>Penicillium chrysogenum - classification</subject><subject>Penicillium chrysogenum - genetics</subject><subject>Phylogeny</subject><subject>Sequence Analysis, DNA</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0LtOwzAUAFALgWgpjKzIA2ILXL_yGFHFS0ICCZgj13FaI8cJcTxk4qP4D74Jt7SsTLZ8z30ZoVMClwQgu_IANOcABEhe7KEp4YwmUBRkH02BcZbkqSATdOT9e0S0AHKIJoQQlhLGpujzZeiDGkIvLZauwt1qtO1SOz0YFR-kHb3xuK3xsNL4-yuRajAOa6fayrglXktc922ziXfaGWWsNS7p-rYKak3q4JbB4-ddLDRYrfrRr7uE5hgd1NJ6fbI9Z-jt9uZ1fp88Pt09zK8fE0U5GxJGOS0YLFRcLBUikzkjPM1zWWe8UjKjSqSFFFlaxRsTRDFJo9OsEqTKOWczdPFbNw72EbQfysZ4pa2VTrfBlxnEZA7sX0iyTABsKp5tYVg0uiq73jSyH8vd30ZwvgXSK2nrXjpl_J-LA1OAlP0AdKuJfw</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>DIEZ, Bruno</creator><creator>MARCOS, Ana Teresa</creator><creator>RODRIGUEZ, Marta</creator><creator>DE LA FUENTE, Juan Luis</creator><creator>BARREDO, José Luis</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20010201</creationdate><title>Structural and phylogenetic analysis of the γ-actin encoding gene from the penicillin-producing fungus Penicillium chrysogenum</title><author>DIEZ, Bruno ; MARCOS, Ana Teresa ; RODRIGUEZ, Marta ; DE LA FUENTE, Juan Luis ; BARREDO, José Luis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c243t-3242930bc9916557a8314688af74dca72c569a576d2c5351c3a2655e3d51d8443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>5' Untranslated Regions</topic><topic>Actins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>g-Actin</topic><topic>Gene Dosage</topic><topic>Genes, Fungal</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Penicillium chrysogenum</topic><topic>Penicillium chrysogenum - classification</topic><topic>Penicillium chrysogenum - genetics</topic><topic>Phylogeny</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DIEZ, Bruno</creatorcontrib><creatorcontrib>MARCOS, Ana Teresa</creatorcontrib><creatorcontrib>RODRIGUEZ, Marta</creatorcontrib><creatorcontrib>DE LA FUENTE, Juan Luis</creatorcontrib><creatorcontrib>BARREDO, José Luis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DIEZ, Bruno</au><au>MARCOS, Ana Teresa</au><au>RODRIGUEZ, Marta</au><au>DE LA FUENTE, Juan Luis</au><au>BARREDO, José Luis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural and phylogenetic analysis of the γ-actin encoding gene from the penicillin-producing fungus Penicillium chrysogenum</atitle><jtitle>Current microbiology</jtitle><addtitle>Curr Microbiol</addtitle><date>2001-02-01</date><risdate>2001</risdate><volume>42</volume><issue>2</issue><spage>117</spage><epage>121</epage><pages>117-121</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><coden>CUMIDD</coden><abstract>The nucleotide sequence of a 2994-bp genomic fragment, including the gamma-actin encoding gene from Penicillium chrysogenum, has been determined, showing an open reading frame (ORF) of 1756 bp interrupted by five introns with fungal consensus splice-site junctions. The 5' untranslated region contains a consensus TATA box, five CAAT motifs, and two large pyrimidine stretches. The predicted protein (375 amino acids) revealed high identity to gamma-actins from fungi (>90%), and gene phylogenies support the grouping of P. chrysogenum actin close to those from the majority of the filamentous fungi. The actA gene is present as a single copy in the genome of P. chrysogenum, and its expression is constitutive during penicillin fermentation, showing a single 1.4-kb transcript.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>11136133</pmid><doi>10.1007/s002840010189</doi><tpages>5</tpages></addata></record> |
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| subjects | 5' Untranslated Regions Actins - genetics Bacteriology Biological and medical sciences Cloning, Molecular Fundamental and applied biological sciences. Psychology g-Actin Gene Dosage Genes, Fungal Genetics Microbiology Molecular Sequence Data Penicillium chrysogenum Penicillium chrysogenum - classification Penicillium chrysogenum - genetics Phylogeny Sequence Analysis, DNA |
| title | Structural and phylogenetic analysis of the γ-actin encoding gene from the penicillin-producing fungus Penicillium chrysogenum |
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