Oriented immobilization of epidermal growth factor onto culture substrates for the selective expansion of neural stem cells

Abstract To develop a culture substrate that allows efficient expansion of neural stem cells (NSCs), epidermal growth factor (EGF) was immobilized onto the Ni(II)-chelated surface of a glass-based substrate through coordination of Ni(II) to the histidine tag that was fused to the C-terminal of EGF u...

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