Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF
The study aimed to establish zona pellucida induced acrosome reaction response (ZIAR) among 35 couples with normal and G-pattern sperm morphology and repeated poor fertilization results during assisted reproduction treatment. ZIAR tests were performed using 0.25 zona pellucida/μl co-incubated with s...
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| Veröffentlicht in: | Human reproduction (Oxford) 2001-01, Vol.16 (1), p.138-144 |
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| creator | Esterhuizen, A.D. Franken, D.R. Lourens, J.G.H. van Rooyen, L.H. |
| description | The study aimed to establish zona pellucida induced acrosome reaction response (ZIAR) among 35 couples with normal and G-pattern sperm morphology and repeated poor fertilization results during assisted reproduction treatment. ZIAR tests were performed using 0.25 zona pellucida/μl co-incubated with spermatozoa for 60 min. Acrosome reactions were measured with FITC–PSA staining, and expressed as the difference between stimulated and unstimulated (spontaneous) sperm populations. Results were compared with IVF rates of metaphase II oocytes. Interactive dot diagrams divided the patients into two groups, i.e. ZIAR 15%, with mean fertilization rates of 49 and 79% respectively. The sensitivity and specificity for ZIAR results versus fertilization were 93 and 100% respectively. The area under the curve was 99% and the 95% confidence interval did not include 0.5 which implies that the ZIAR test is able to predict fertilization failure among IVF patients. In conclusion, the ZIAR test has diagnostic potential since it can assist the clinician to identify couples that will benefit from intracytoplasmic sperm injection therapy. |
| doi_str_mv | 10.1093/humrep/16.1.138 |
| format | Article |
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ZIAR tests were performed using 0.25 zona pellucida/μl co-incubated with spermatozoa for 60 min. Acrosome reactions were measured with FITC–PSA staining, and expressed as the difference between stimulated and unstimulated (spontaneous) sperm populations. Results were compared with IVF rates of metaphase II oocytes. Interactive dot diagrams divided the patients into two groups, i.e. ZIAR <15% and ZIAR > 15%, with mean fertilization rates of 49 and 79% respectively. The sensitivity and specificity for ZIAR results versus fertilization were 93 and 100% respectively. The area under the curve was 99% and the 95% confidence interval did not include 0.5 which implies that the ZIAR test is able to predict fertilization failure among IVF patients. In conclusion, the ZIAR test has diagnostic potential since it can assist the clinician to identify couples that will benefit from intracytoplasmic sperm injection therapy.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/humrep/16.1.138</identifier><identifier>PMID: 11139552</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>acrosome reaction ; Acrosome Reaction - physiology ; Adult ; Biological and medical sciences ; failed fertilization ; Female ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; Humans ; Infertility, Female - diagnosis ; Infertility, Female - physiopathology ; Infertility, Female - therapy ; Male ; Mammalian reproduction. General aspects ; Predictive Value of Tests ; ROC Curve ; Sperm Count ; Sperm Injections, Intracytoplasmic ; Sperm Motility ; Treatment Failure ; Vertebrates: reproduction ; zona pellucida ; Zona Pellucida - physiology</subject><ispartof>Human reproduction (Oxford), 2001-01, Vol.16 (1), p.138-144</ispartof><rights>European Society of Human Reproduction and Embryology 2001</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-1a973a48734ca8ab9280b67a31fb6a5e3d6c6a1e0f24356648c16cff8bc23dc23</citedby><cites>FETCH-LOGICAL-c435t-1a973a48734ca8ab9280b67a31fb6a5e3d6c6a1e0f24356648c16cff8bc23dc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1583,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=855426$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11139552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Esterhuizen, A.D.</creatorcontrib><creatorcontrib>Franken, D.R.</creatorcontrib><creatorcontrib>Lourens, J.G.H.</creatorcontrib><creatorcontrib>van Rooyen, L.H.</creatorcontrib><title>Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF</title><title>Human reproduction (Oxford)</title><addtitle>Hum. Reprod</addtitle><addtitle>Hum. Reprod</addtitle><description>The study aimed to establish zona pellucida induced acrosome reaction response (ZIAR) among 35 couples with normal and G-pattern sperm morphology and repeated poor fertilization results during assisted reproduction treatment. ZIAR tests were performed using 0.25 zona pellucida/μl co-incubated with spermatozoa for 60 min. Acrosome reactions were measured with FITC–PSA staining, and expressed as the difference between stimulated and unstimulated (spontaneous) sperm populations. Results were compared with IVF rates of metaphase II oocytes. Interactive dot diagrams divided the patients into two groups, i.e. ZIAR <15% and ZIAR > 15%, with mean fertilization rates of 49 and 79% respectively. The sensitivity and specificity for ZIAR results versus fertilization were 93 and 100% respectively. The area under the curve was 99% and the 95% confidence interval did not include 0.5 which implies that the ZIAR test is able to predict fertilization failure among IVF patients. In conclusion, the ZIAR test has diagnostic potential since it can assist the clinician to identify couples that will benefit from intracytoplasmic sperm injection therapy.</description><subject>acrosome reaction</subject><subject>Acrosome Reaction - physiology</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>failed fertilization</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Infertility, Female - diagnosis</subject><subject>Infertility, Female - physiopathology</subject><subject>Infertility, Female - therapy</subject><subject>Male</subject><subject>Mammalian reproduction. General aspects</subject><subject>Predictive Value of Tests</subject><subject>ROC Curve</subject><subject>Sperm Count</subject><subject>Sperm Injections, Intracytoplasmic</subject><subject>Sperm Motility</subject><subject>Treatment Failure</subject><subject>Vertebrates: reproduction</subject><subject>zona pellucida</subject><subject>Zona Pellucida - physiology</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0E1r3DAQBmBRWppN2nNvQVDoIeBdjWXJ2mNYmg8I5NA2hF7UsSwRtbblSHZo--uj4CW55iBGiGdGzEvIJ2BrYFu-uZv7aMcNyDWsgas3ZAWVZEXJBXtLVqyUqgCQcEAOU_rNWL4q-Z4cAADfClGuyK9d5wdvsKO-H0OccDCWBkf_hwHpaLtuNr7Fwg_tbGxL0cSQQm9ptGgmHwaKQ0v9lOgYbevz04OlD9jNlroQ6eXN2QfyzmGX7Md9PSI_zr5-310UV9fnl7vTq8JUXEwF4LbmWKmaVwYVNttSsUbWyME1EoXlrTQSwTJXZi9lpQxI45xqTMnbfI7Il2XuGMP9bNOke59MXgAHG-akayZqIRXPcLPAp1VStE6P0fcY_2lg-ilUvYSqQWrQOdTccbwfPTe9bV_8PsUMPu8BphylizlFn56dEqIqZVYniwrz-Io_iwX7NNm_zxzjHy1rXgt9cftTf1NwLm5vSl3xR6icnls</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Esterhuizen, A.D.</creator><creator>Franken, D.R.</creator><creator>Lourens, J.G.H.</creator><creator>van Rooyen, L.H.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200101</creationdate><title>Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF</title><author>Esterhuizen, A.D. ; Franken, D.R. ; Lourens, J.G.H. ; van Rooyen, L.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-1a973a48734ca8ab9280b67a31fb6a5e3d6c6a1e0f24356648c16cff8bc23dc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>acrosome reaction</topic><topic>Acrosome Reaction - physiology</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>failed fertilization</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Infertility, Female - diagnosis</topic><topic>Infertility, Female - physiopathology</topic><topic>Infertility, Female - therapy</topic><topic>Male</topic><topic>Mammalian reproduction. General aspects</topic><topic>Predictive Value of Tests</topic><topic>ROC Curve</topic><topic>Sperm Count</topic><topic>Sperm Injections, Intracytoplasmic</topic><topic>Sperm Motility</topic><topic>Treatment Failure</topic><topic>Vertebrates: reproduction</topic><topic>zona pellucida</topic><topic>Zona Pellucida - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Esterhuizen, A.D.</creatorcontrib><creatorcontrib>Franken, D.R.</creatorcontrib><creatorcontrib>Lourens, J.G.H.</creatorcontrib><creatorcontrib>van Rooyen, L.H.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Esterhuizen, A.D.</au><au>Franken, D.R.</au><au>Lourens, J.G.H.</au><au>van Rooyen, L.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF</atitle><jtitle>Human reproduction (Oxford)</jtitle><stitle>Hum. Reprod</stitle><addtitle>Hum. Reprod</addtitle><date>2001-01</date><risdate>2001</risdate><volume>16</volume><issue>1</issue><spage>138</spage><epage>144</epage><pages>138-144</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>The study aimed to establish zona pellucida induced acrosome reaction response (ZIAR) among 35 couples with normal and G-pattern sperm morphology and repeated poor fertilization results during assisted reproduction treatment. ZIAR tests were performed using 0.25 zona pellucida/μl co-incubated with spermatozoa for 60 min. Acrosome reactions were measured with FITC–PSA staining, and expressed as the difference between stimulated and unstimulated (spontaneous) sperm populations. Results were compared with IVF rates of metaphase II oocytes. Interactive dot diagrams divided the patients into two groups, i.e. ZIAR <15% and ZIAR > 15%, with mean fertilization rates of 49 and 79% respectively. The sensitivity and specificity for ZIAR results versus fertilization were 93 and 100% respectively. The area under the curve was 99% and the 95% confidence interval did not include 0.5 which implies that the ZIAR test is able to predict fertilization failure among IVF patients. In conclusion, the ZIAR test has diagnostic potential since it can assist the clinician to identify couples that will benefit from intracytoplasmic sperm injection therapy.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>11139552</pmid><doi>10.1093/humrep/16.1.138</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | acrosome reaction Acrosome Reaction - physiology Adult Biological and medical sciences failed fertilization Female Fertilization in Vitro Fundamental and applied biological sciences. Psychology Humans Infertility, Female - diagnosis Infertility, Female - physiopathology Infertility, Female - therapy Male Mammalian reproduction. General aspects Predictive Value of Tests ROC Curve Sperm Count Sperm Injections, Intracytoplasmic Sperm Motility Treatment Failure Vertebrates: reproduction zona pellucida Zona Pellucida - physiology |
| title | Clinical importance of zona pellucida-induced acrosome reaction and its predictive value for IVF |
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