Serum level and tissue expression of c-erbB-1 and c-erbB-2 proto-oncogene products in patients with squamous cell carcinoma of the head and neck
The proto-oncogene products erbB-l (EGF-Receptor) and erbB-2 (HER-2/ neu), distinct members of the epidermal growth factor receptor family, are frequently overexpressed in squamous cell carcinoma of the head and neck (SCCHN). The accumulation of these transmembrane proteins may lead to significant a...
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| Veröffentlicht in: | Oral oncology 2001, Vol.37 (1), p.50-56 |
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| creator | Hoffmann, T.K Balló, H Braunstein, S Van Lierop, A Wagenmann, M Bier, H |
| description | The proto-oncogene products erbB-l (EGF-Receptor) and erbB-2 (HER-2/
neu), distinct members of the epidermal growth factor receptor family, are frequently overexpressed in squamous cell carcinoma of the head and neck (SCCHN). The accumulation of these transmembrane proteins may lead to significant amounts of the respective extracellular receptor domains (ECD) that are shed from the tumour cell surface and enter blood circulation, thus representing potential serum tumour markers. For erbB-l and erbB-2, we determined the ECD serum levels with enzyme-linked immunosorbent assays and evaluated the protein expression in tumour tissue by immunohistochemistry. The present study included 49 patients (37 untreated, 12 recurrences) and the same number of age- and sex-matched healthy controls. In 24 patients ECD serum levels were determined before and 6 weeks after surgery. Mean ECD serum levels for erbB-1 and erbB-2 were 54.8±1.6 and 153.7±6.1 fmol/ml in cancer patients, and 54±1.5 and 147.9±4.5 fmol/ml in healthy controls, respectively. There was no significant difference between untreated and recurrent disease. Serum ECD follow-ups 6 weeks after surgery revealed a significant 12.3% decline of erbB-1 but no change of erbB-2 values. Immunohistochemistry showed strong staining for erbB-1 in 78% and erbB-2 in 47% of the SCCHN specimens. No correlation was detectable between receptor ECD serum levels and receptor tissue expression, tumour stage, and tumour differentiation. Hence, ECD serum levels of erbB-1 and erbB-2 are not considered to be valuable tumour markers in SCCHN. |
| doi_str_mv | 10.1016/S1368-8375(00)00056-7 |
| format | Article |
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neu), distinct members of the epidermal growth factor receptor family, are frequently overexpressed in squamous cell carcinoma of the head and neck (SCCHN). The accumulation of these transmembrane proteins may lead to significant amounts of the respective extracellular receptor domains (ECD) that are shed from the tumour cell surface and enter blood circulation, thus representing potential serum tumour markers. For erbB-l and erbB-2, we determined the ECD serum levels with enzyme-linked immunosorbent assays and evaluated the protein expression in tumour tissue by immunohistochemistry. The present study included 49 patients (37 untreated, 12 recurrences) and the same number of age- and sex-matched healthy controls. In 24 patients ECD serum levels were determined before and 6 weeks after surgery. Mean ECD serum levels for erbB-1 and erbB-2 were 54.8±1.6 and 153.7±6.1 fmol/ml in cancer patients, and 54±1.5 and 147.9±4.5 fmol/ml in healthy controls, respectively. There was no significant difference between untreated and recurrent disease. Serum ECD follow-ups 6 weeks after surgery revealed a significant 12.3% decline of erbB-1 but no change of erbB-2 values. Immunohistochemistry showed strong staining for erbB-1 in 78% and erbB-2 in 47% of the SCCHN specimens. No correlation was detectable between receptor ECD serum levels and receptor tissue expression, tumour stage, and tumour differentiation. Hence, ECD serum levels of erbB-1 and erbB-2 are not considered to be valuable tumour markers in SCCHN.</description><identifier>ISSN: 1368-8375</identifier><identifier>EISSN: 1879-0593</identifier><identifier>DOI: 10.1016/S1368-8375(00)00056-7</identifier><identifier>PMID: 11120483</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biological and medical sciences ; Biomarkers, Tumor - blood ; Biomarkers, Tumor - metabolism ; c-erbB-1 ; c-erbB-2 ; Carcinoma, Squamous Cell - blood ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Ent. Stomatology ; Enzyme-Linked Immunosorbent Assay ; Female ; Follow-Up Studies ; Head and neck cancer ; Head and Neck Neoplasms - blood ; Head and Neck Neoplasms - metabolism ; Head and Neck Neoplasms - pathology ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Male ; Medical sciences ; Middle Aged ; Neoplasm Proteins - blood ; Neoplasm Proteins - metabolism ; Neoplasm Staging ; Otorhinolaryngology (head neck, general aspects and miscellaneous) ; Otorhinolaryngology. Stomatology ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Receptor, Epidermal Growth Factor - blood ; Receptor, Epidermal Growth Factor - metabolism ; Receptor, ErbB-2 - blood ; Receptor, ErbB-2 - metabolism ; Tumors ; Tumour marker</subject><ispartof>Oral oncology, 2001, Vol.37 (1), p.50-56</ispartof><rights>2001 Elsevier Science Ltd</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c304t-e880c1171049c55d4b809e681a8697e67031c1564d74c008254bc31d4fc07fae3</citedby><cites>FETCH-LOGICAL-c304t-e880c1171049c55d4b809e681a8697e67031c1564d74c008254bc31d4fc07fae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1368-8375(00)00056-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,4012,27906,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=860459$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11120483$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoffmann, T.K</creatorcontrib><creatorcontrib>Balló, H</creatorcontrib><creatorcontrib>Braunstein, S</creatorcontrib><creatorcontrib>Van Lierop, A</creatorcontrib><creatorcontrib>Wagenmann, M</creatorcontrib><creatorcontrib>Bier, H</creatorcontrib><title>Serum level and tissue expression of c-erbB-1 and c-erbB-2 proto-oncogene products in patients with squamous cell carcinoma of the head and neck</title><title>Oral oncology</title><addtitle>Oral Oncol</addtitle><description>The proto-oncogene products erbB-l (EGF-Receptor) and erbB-2 (HER-2/
neu), distinct members of the epidermal growth factor receptor family, are frequently overexpressed in squamous cell carcinoma of the head and neck (SCCHN). The accumulation of these transmembrane proteins may lead to significant amounts of the respective extracellular receptor domains (ECD) that are shed from the tumour cell surface and enter blood circulation, thus representing potential serum tumour markers. For erbB-l and erbB-2, we determined the ECD serum levels with enzyme-linked immunosorbent assays and evaluated the protein expression in tumour tissue by immunohistochemistry. The present study included 49 patients (37 untreated, 12 recurrences) and the same number of age- and sex-matched healthy controls. In 24 patients ECD serum levels were determined before and 6 weeks after surgery. Mean ECD serum levels for erbB-1 and erbB-2 were 54.8±1.6 and 153.7±6.1 fmol/ml in cancer patients, and 54±1.5 and 147.9±4.5 fmol/ml in healthy controls, respectively. There was no significant difference between untreated and recurrent disease. Serum ECD follow-ups 6 weeks after surgery revealed a significant 12.3% decline of erbB-1 but no change of erbB-2 values. Immunohistochemistry showed strong staining for erbB-1 in 78% and erbB-2 in 47% of the SCCHN specimens. No correlation was detectable between receptor ECD serum levels and receptor tissue expression, tumour stage, and tumour differentiation. Hence, ECD serum levels of erbB-1 and erbB-2 are not considered to be valuable tumour markers in SCCHN.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - blood</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>c-erbB-1</subject><subject>c-erbB-2</subject><subject>Carcinoma, Squamous Cell - blood</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Ent. Stomatology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Head and neck cancer</subject><subject>Head and Neck Neoplasms - blood</subject><subject>Head and Neck Neoplasms - metabolism</subject><subject>Head and Neck Neoplasms - pathology</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Neoplasm Proteins - blood</subject><subject>Neoplasm Proteins - metabolism</subject><subject>Neoplasm Staging</subject><subject>Otorhinolaryngology (head neck, general aspects and miscellaneous)</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Receptor, Epidermal Growth Factor - blood</subject><subject>Receptor, Epidermal Growth Factor - metabolism</subject><subject>Receptor, ErbB-2 - blood</subject><subject>Receptor, ErbB-2 - metabolism</subject><subject>Tumors</subject><subject>Tumour marker</subject><issn>1368-8375</issn><issn>1879-0593</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O1DAQhC0EYpeBRwBZQkJwCLQn8U9OCFb8SStxWDhbnnaHMST2rJ0s8BY8MslMgCMnd0tfl0tVjD0U8FyAUC-uRK1MZWotnwI8AwCpKn2LnQuj2wpkW9-e5z_IGbtXytcFEhLusjMhxBYaU5-zX1eUp4H3dEM9d9HzMZQyEacfh0ylhBR56jhWlHevK3Ek1mXLDzmNqUoR0xeKtKx-wrHwEPnBjYHiPH8P456X68kNaSocqe85uowhpsEtyuOe-J6cPypHwm_32Z3O9YUerO-GfX775tPF--ry47sPF68uK6yhGSsyBlAILaBpUUrf7Ay0pIxwRrWalIZaoJCq8bpBALOVzQ5r4ZsOQXeO6g17ctKdbV9PVEY7hLL4c5Fmq1aD1FKBnkF5AjGnUjJ19pDD4PJPK8AuVdhjFXbJ2QLYYxV2uXu0fjDtBvL_rtbsZ-DxCriCru-yixjKX84oaOYaN-zliaI5jJtA2Raco0XyIROO1qfwHyO_AW-xpR8</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Hoffmann, T.K</creator><creator>Balló, H</creator><creator>Braunstein, S</creator><creator>Van Lierop, A</creator><creator>Wagenmann, M</creator><creator>Bier, H</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>Serum level and tissue expression of c-erbB-1 and c-erbB-2 proto-oncogene products in patients with squamous cell carcinoma of the head and neck</title><author>Hoffmann, T.K ; Balló, H ; Braunstein, S ; Van Lierop, A ; Wagenmann, M ; Bier, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c304t-e880c1171049c55d4b809e681a8697e67031c1564d74c008254bc31d4fc07fae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - blood</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>c-erbB-1</topic><topic>c-erbB-2</topic><topic>Carcinoma, Squamous Cell - blood</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Ent. Stomatology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Head and neck cancer</topic><topic>Head and Neck Neoplasms - blood</topic><topic>Head and Neck Neoplasms - metabolism</topic><topic>Head and Neck Neoplasms - pathology</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Neoplasm Proteins - blood</topic><topic>Neoplasm Proteins - metabolism</topic><topic>Neoplasm Staging</topic><topic>Otorhinolaryngology (head neck, general aspects and miscellaneous)</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Receptor, Epidermal Growth Factor - blood</topic><topic>Receptor, Epidermal Growth Factor - metabolism</topic><topic>Receptor, ErbB-2 - blood</topic><topic>Receptor, ErbB-2 - metabolism</topic><topic>Tumors</topic><topic>Tumour marker</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoffmann, T.K</creatorcontrib><creatorcontrib>Balló, H</creatorcontrib><creatorcontrib>Braunstein, S</creatorcontrib><creatorcontrib>Van Lierop, A</creatorcontrib><creatorcontrib>Wagenmann, M</creatorcontrib><creatorcontrib>Bier, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Oral oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoffmann, T.K</au><au>Balló, H</au><au>Braunstein, S</au><au>Van Lierop, A</au><au>Wagenmann, M</au><au>Bier, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum level and tissue expression of c-erbB-1 and c-erbB-2 proto-oncogene products in patients with squamous cell carcinoma of the head and neck</atitle><jtitle>Oral oncology</jtitle><addtitle>Oral Oncol</addtitle><date>2001</date><risdate>2001</risdate><volume>37</volume><issue>1</issue><spage>50</spage><epage>56</epage><pages>50-56</pages><issn>1368-8375</issn><eissn>1879-0593</eissn><abstract>The proto-oncogene products erbB-l (EGF-Receptor) and erbB-2 (HER-2/
neu), distinct members of the epidermal growth factor receptor family, are frequently overexpressed in squamous cell carcinoma of the head and neck (SCCHN). The accumulation of these transmembrane proteins may lead to significant amounts of the respective extracellular receptor domains (ECD) that are shed from the tumour cell surface and enter blood circulation, thus representing potential serum tumour markers. For erbB-l and erbB-2, we determined the ECD serum levels with enzyme-linked immunosorbent assays and evaluated the protein expression in tumour tissue by immunohistochemistry. The present study included 49 patients (37 untreated, 12 recurrences) and the same number of age- and sex-matched healthy controls. In 24 patients ECD serum levels were determined before and 6 weeks after surgery. Mean ECD serum levels for erbB-1 and erbB-2 were 54.8±1.6 and 153.7±6.1 fmol/ml in cancer patients, and 54±1.5 and 147.9±4.5 fmol/ml in healthy controls, respectively. There was no significant difference between untreated and recurrent disease. Serum ECD follow-ups 6 weeks after surgery revealed a significant 12.3% decline of erbB-1 but no change of erbB-2 values. Immunohistochemistry showed strong staining for erbB-1 in 78% and erbB-2 in 47% of the SCCHN specimens. No correlation was detectable between receptor ECD serum levels and receptor tissue expression, tumour stage, and tumour differentiation. Hence, ECD serum levels of erbB-1 and erbB-2 are not considered to be valuable tumour markers in SCCHN.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>11120483</pmid><doi>10.1016/S1368-8375(00)00056-7</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged Aged, 80 and over Biological and medical sciences Biomarkers, Tumor - blood Biomarkers, Tumor - metabolism c-erbB-1 c-erbB-2 Carcinoma, Squamous Cell - blood Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Ent. Stomatology Enzyme-Linked Immunosorbent Assay Female Follow-Up Studies Head and neck cancer Head and Neck Neoplasms - blood Head and Neck Neoplasms - metabolism Head and Neck Neoplasms - pathology Humans Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Neoplasm Proteins - blood Neoplasm Proteins - metabolism Neoplasm Staging Otorhinolaryngology (head neck, general aspects and miscellaneous) Otorhinolaryngology. Stomatology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Receptor, Epidermal Growth Factor - blood Receptor, Epidermal Growth Factor - metabolism Receptor, ErbB-2 - blood Receptor, ErbB-2 - metabolism Tumors Tumour marker |
| title | Serum level and tissue expression of c-erbB-1 and c-erbB-2 proto-oncogene products in patients with squamous cell carcinoma of the head and neck |
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