Heterogeneity of the Memory CD4 T Cell Response: Persisting Effectors and Resting Memory T Cells
Defining the cellular composition of the memory T cell pool has been complicated by an inability to distinguish effector and memory T cells. We present here an activation profile assay, using anti-CD3 and antigenic stimuli, that clearly distinguishes effector and memory CD4 T cells and defines subse...
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| Veröffentlicht in: | The Journal of immunology (1950) 2001-01, Vol.166 (2), p.926-935 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 166 |
| creator | Ahmadzadeh, Mojgan Hussain, S. Farzana Farber, Donna L |
| description | Defining the cellular composition of the memory T cell pool has been complicated by an inability to distinguish effector and memory T cells. We present here an activation profile assay, using anti-CD3 and antigenic stimuli, that clearly distinguishes effector and memory CD4 T cells and defines subsets of long-lived memory CD4 T cells based on CD62 ligand (CD62L) expression. The CD62L(low) memory subset functionally resembles effector cells, exhibiting hyper-responsiveness to antigenic and anti-CD3 mediated stimuli, high proliferative capacity, and rapid activation kinetics. The CD62L(high) memory subset functionally resembles resting memory cells, exhibiting hyporesponsiveness to anti-CD3 stimuli, lower proliferative capacity, and slower activation kinetics. Our results indicate that the memory CD4 T cell pool is heterogeneous, consisting of persisting effectors and resting memory T cells. |
| doi_str_mv | 10.4049/jimmunol.166.2.926 |
| format | Article |
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The CD62L(high) memory subset functionally resembles resting memory cells, exhibiting hyporesponsiveness to anti-CD3 stimuli, lower proliferative capacity, and slower activation kinetics. 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The CD62L(low) memory subset functionally resembles effector cells, exhibiting hyper-responsiveness to antigenic and anti-CD3 mediated stimuli, high proliferative capacity, and rapid activation kinetics. The CD62L(high) memory subset functionally resembles resting memory cells, exhibiting hyporesponsiveness to anti-CD3 stimuli, lower proliferative capacity, and slower activation kinetics. Our results indicate that the memory CD4 T cell pool is heterogeneous, consisting of persisting effectors and resting memory T cells.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>11145669</pmid><doi>10.4049/jimmunol.166.2.926</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 2001-01, Vol.166 (2), p.926-935 |
| issn | 0022-1767 1550-6606 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adoptive Transfer Animals CD4-Positive T-Lymphocytes - cytology CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism CD4-Positive T-Lymphocytes - transplantation CD62L protein Cell Separation Cell Survival - immunology Cytokines - biosynthesis Epitopes, T-Lymphocyte - immunology Flow Cytometry Hemagglutinin Glycoproteins, Influenza Virus - immunology Histocompatibility Antigens Class II Immunologic Memory Interphase - immunology L-Selectin - biosynthesis Lymphocyte Activation Mice Mice, Inbred BALB C Mice, Knockout Mice, Transgenic Receptors, Antigen, T-Cell - genetics Receptors, Antigen, T-Cell - physiology T-Lymphocyte Subsets - cytology T-Lymphocyte Subsets - immunology T-Lymphocyte Subsets - metabolism T-Lymphocyte Subsets - transplantation |
| title | Heterogeneity of the Memory CD4 T Cell Response: Persisting Effectors and Resting Memory T Cells |
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