Adipophilin expression is increased in symptomatic carotid atherosclerosis : Correlation with red blood cells and cholesterol crystals
Adipophilin is an adipose differentiation-related protein expressed in lipid-containing cells. Using DNA microarray analysis, we previously found the adipophilin gene (ADFP) to be overexpressed in symptomatic carotid plaques (CP). This led us to further examine the role of adipophilin in carotid ath...
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| Veröffentlicht in: | Stroke (1970) 2007-06, Vol.38 (6), p.1791-1798 |
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| creator | NUOTIO, Krista ISOVIITA, Pia M KASTE, Markku LINDSBERG, Perttu J SAKSI, Jani IJÄS, Petra PITKÄNIEMI, Janne SONNINEN, Riitta SOINNE, Lauri SAIMANEN, Eija SALONEN, Oili KOVANEN, Petri T |
| description | Adipophilin is an adipose differentiation-related protein expressed in lipid-containing cells. Using DNA microarray analysis, we previously found the adipophilin gene (ADFP) to be overexpressed in symptomatic carotid plaques (CP). This led us to further examine the role of adipophilin in carotid atherosclerosis relative to symptom status.
Ninety-eight high-grade (>70%) CPs were obtained in carotid endarterectomy. The relative expression of ADFP mRNA was measured by quantitative real-time RT-PCR, and the relative amount of adipophilin protein was quantified with Western blotting. Detailed topographical correlations with extravasated red blood cells and extracellular cholesterol crystals were obtained by means of immunohistochemistry.
The relative expression of ADFP mRNA was increased in symptomatic compared with asymptomatic CPs at both the mRNA level (1.82+/-0.19[SE] versus 1.25+/-0.15, P=0.012) and the protein level (1.04+/-0.23 versus 0.46+/-0.14, P=0.043). Adipophilin colocalized with macrophage foam cells, extravasated red blood cells (P |
| doi_str_mv | 10.1161/STROKEAHA.106.478867 |
| format | Article |
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Ninety-eight high-grade (>70%) CPs were obtained in carotid endarterectomy. The relative expression of ADFP mRNA was measured by quantitative real-time RT-PCR, and the relative amount of adipophilin protein was quantified with Western blotting. Detailed topographical correlations with extravasated red blood cells and extracellular cholesterol crystals were obtained by means of immunohistochemistry.
The relative expression of ADFP mRNA was increased in symptomatic compared with asymptomatic CPs at both the mRNA level (1.82+/-0.19[SE] versus 1.25+/-0.15, P=0.012) and the protein level (1.04+/-0.23 versus 0.46+/-0.14, P=0.043). Adipophilin colocalized with macrophage foam cells, extravasated red blood cells (P<0.0001), and cholesterol crystals (P<0.0001), and its expression associated with macroscopic ulceration of CP (P<0.0001).
Intraplaque hemorrhages may contribute to intracellular lipid accumulation and consequent adipophilin expression. Because adipophilin blocks cholesterol efflux from lipid-laden cells, they may die and develop a necrotic lipid core, thereby destabilizing the plaque.</description><identifier>ISSN: 0039-2499</identifier><identifier>EISSN: 1524-4628</identifier><identifier>DOI: 10.1161/STROKEAHA.106.478867</identifier><identifier>PMID: 17446422</identifier><identifier>CODEN: SJCCA7</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Aged ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Carotid Artery Diseases - genetics ; Carotid Artery Diseases - metabolism ; Carotid Artery Diseases - pathology ; Carotid Artery, Internal - metabolism ; Carotid Artery, Internal - pathology ; Cholesterol - analysis ; Cholesterol - metabolism ; Crystallization ; Erythrocytes - metabolism ; Female ; Gene Expression Regulation - physiology ; Humans ; Male ; Medical sciences ; Membrane Proteins ; Middle Aged ; Necrosis ; Neurology ; Neurosurgery ; Peptides - genetics ; Peptides - metabolism ; Perilipin-2 ; Skull, brain, vascular surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Vascular diseases and vascular malformations of the nervous system</subject><ispartof>Stroke (1970), 2007-06, Vol.38 (6), p.1791-1798</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c284t-c40aec99cb5fb22522c4a2f997165c8cac0adf835a77d62b9211bfb1fa35c3ce3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3673,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18808186$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17446422$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NUOTIO, Krista</creatorcontrib><creatorcontrib>ISOVIITA, Pia M</creatorcontrib><creatorcontrib>KASTE, Markku</creatorcontrib><creatorcontrib>LINDSBERG, Perttu J</creatorcontrib><creatorcontrib>SAKSI, Jani</creatorcontrib><creatorcontrib>IJÄS, Petra</creatorcontrib><creatorcontrib>PITKÄNIEMI, Janne</creatorcontrib><creatorcontrib>SONNINEN, Riitta</creatorcontrib><creatorcontrib>SOINNE, Lauri</creatorcontrib><creatorcontrib>SAIMANEN, Eija</creatorcontrib><creatorcontrib>SALONEN, Oili</creatorcontrib><creatorcontrib>KOVANEN, Petri T</creatorcontrib><title>Adipophilin expression is increased in symptomatic carotid atherosclerosis : Correlation with red blood cells and cholesterol crystals</title><title>Stroke (1970)</title><addtitle>Stroke</addtitle><description>Adipophilin is an adipose differentiation-related protein expressed in lipid-containing cells. Using DNA microarray analysis, we previously found the adipophilin gene (ADFP) to be overexpressed in symptomatic carotid plaques (CP). This led us to further examine the role of adipophilin in carotid atherosclerosis relative to symptom status.
Ninety-eight high-grade (>70%) CPs were obtained in carotid endarterectomy. The relative expression of ADFP mRNA was measured by quantitative real-time RT-PCR, and the relative amount of adipophilin protein was quantified with Western blotting. Detailed topographical correlations with extravasated red blood cells and extracellular cholesterol crystals were obtained by means of immunohistochemistry.
The relative expression of ADFP mRNA was increased in symptomatic compared with asymptomatic CPs at both the mRNA level (1.82+/-0.19[SE] versus 1.25+/-0.15, P=0.012) and the protein level (1.04+/-0.23 versus 0.46+/-0.14, P=0.043). Adipophilin colocalized with macrophage foam cells, extravasated red blood cells (P<0.0001), and cholesterol crystals (P<0.0001), and its expression associated with macroscopic ulceration of CP (P<0.0001).
Intraplaque hemorrhages may contribute to intracellular lipid accumulation and consequent adipophilin expression. Because adipophilin blocks cholesterol efflux from lipid-laden cells, they may die and develop a necrotic lipid core, thereby destabilizing the plaque.</description><subject>Aged</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Carotid Artery Diseases - genetics</subject><subject>Carotid Artery Diseases - metabolism</subject><subject>Carotid Artery Diseases - pathology</subject><subject>Carotid Artery, Internal - metabolism</subject><subject>Carotid Artery, Internal - pathology</subject><subject>Cholesterol - analysis</subject><subject>Cholesterol - metabolism</subject><subject>Crystallization</subject><subject>Erythrocytes - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation - physiology</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane Proteins</subject><subject>Middle Aged</subject><subject>Necrosis</subject><subject>Neurology</subject><subject>Neurosurgery</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Perilipin-2</subject><subject>Skull, brain, vascular surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><issn>0039-2499</issn><issn>1524-4628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFu1DAQhi1ERZfCGyDkC9yytR3HcbitVoUiKlWi5RxNJo7WyImDJ6uyL8Bz16td0cvMaPT9v2Z-xj5IsZbSyOuHx5_3P242t5u1FGata2tN_YqtZKV0oY2yr9lKiLIplG6aS_aW6LcQQpW2esMuZa210Uqt2L9N7-c473zwE3d_5-SIfJy4J-4nTA7I9XnidBjnJY6weOQIKS6-57DsXIqE4Viz4AvfxpRcyFB2ePLLjqes7kKMPUcXAnGY8rSLwdGSRYFjOtACgd6xiyE39_7cr9ivrzeP29vi7v7b9-3mrkBl9VKgFuCwabCrhk6pSinUoIamqaWp0CKggH6wZQV13RvVNUrKbujkAGWFJbryin0--c4p_tnnK9rR0_E0mFzcU1uLyhgtdQb1CcT8GyU3tHPyI6RDK0V7zL_9n3_emPaUf5Z9PPvvu9H1L6Jz4Bn4dAaAEMKQYEJPL5y1wkprymfmdpM_</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>NUOTIO, Krista</creator><creator>ISOVIITA, Pia M</creator><creator>KASTE, Markku</creator><creator>LINDSBERG, Perttu J</creator><creator>SAKSI, Jani</creator><creator>IJÄS, Petra</creator><creator>PITKÄNIEMI, Janne</creator><creator>SONNINEN, Riitta</creator><creator>SOINNE, Lauri</creator><creator>SAIMANEN, Eija</creator><creator>SALONEN, Oili</creator><creator>KOVANEN, Petri T</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070601</creationdate><title>Adipophilin expression is increased in symptomatic carotid atherosclerosis : Correlation with red blood cells and cholesterol crystals</title><author>NUOTIO, Krista ; ISOVIITA, Pia M ; KASTE, Markku ; LINDSBERG, Perttu J ; SAKSI, Jani ; IJÄS, Petra ; PITKÄNIEMI, Janne ; SONNINEN, Riitta ; SOINNE, Lauri ; SAIMANEN, Eija ; SALONEN, Oili ; KOVANEN, Petri T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c284t-c40aec99cb5fb22522c4a2f997165c8cac0adf835a77d62b9211bfb1fa35c3ce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Aged</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Carotid Artery Diseases - genetics</topic><topic>Carotid Artery Diseases - metabolism</topic><topic>Carotid Artery Diseases - pathology</topic><topic>Carotid Artery, Internal - metabolism</topic><topic>Carotid Artery, Internal - pathology</topic><topic>Cholesterol - analysis</topic><topic>Cholesterol - metabolism</topic><topic>Crystallization</topic><topic>Erythrocytes - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation - physiology</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane Proteins</topic><topic>Middle Aged</topic><topic>Necrosis</topic><topic>Neurology</topic><topic>Neurosurgery</topic><topic>Peptides - genetics</topic><topic>Peptides - metabolism</topic><topic>Perilipin-2</topic><topic>Skull, brain, vascular surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NUOTIO, Krista</creatorcontrib><creatorcontrib>ISOVIITA, Pia M</creatorcontrib><creatorcontrib>KASTE, Markku</creatorcontrib><creatorcontrib>LINDSBERG, Perttu J</creatorcontrib><creatorcontrib>SAKSI, Jani</creatorcontrib><creatorcontrib>IJÄS, Petra</creatorcontrib><creatorcontrib>PITKÄNIEMI, Janne</creatorcontrib><creatorcontrib>SONNINEN, Riitta</creatorcontrib><creatorcontrib>SOINNE, Lauri</creatorcontrib><creatorcontrib>SAIMANEN, Eija</creatorcontrib><creatorcontrib>SALONEN, Oili</creatorcontrib><creatorcontrib>KOVANEN, Petri T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Stroke (1970)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NUOTIO, Krista</au><au>ISOVIITA, Pia M</au><au>KASTE, Markku</au><au>LINDSBERG, Perttu J</au><au>SAKSI, Jani</au><au>IJÄS, Petra</au><au>PITKÄNIEMI, Janne</au><au>SONNINEN, Riitta</au><au>SOINNE, Lauri</au><au>SAIMANEN, Eija</au><au>SALONEN, Oili</au><au>KOVANEN, Petri T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adipophilin expression is increased in symptomatic carotid atherosclerosis : Correlation with red blood cells and cholesterol crystals</atitle><jtitle>Stroke (1970)</jtitle><addtitle>Stroke</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>38</volume><issue>6</issue><spage>1791</spage><epage>1798</epage><pages>1791-1798</pages><issn>0039-2499</issn><eissn>1524-4628</eissn><coden>SJCCA7</coden><abstract>Adipophilin is an adipose differentiation-related protein expressed in lipid-containing cells. Using DNA microarray analysis, we previously found the adipophilin gene (ADFP) to be overexpressed in symptomatic carotid plaques (CP). This led us to further examine the role of adipophilin in carotid atherosclerosis relative to symptom status.
Ninety-eight high-grade (>70%) CPs were obtained in carotid endarterectomy. The relative expression of ADFP mRNA was measured by quantitative real-time RT-PCR, and the relative amount of adipophilin protein was quantified with Western blotting. Detailed topographical correlations with extravasated red blood cells and extracellular cholesterol crystals were obtained by means of immunohistochemistry.
The relative expression of ADFP mRNA was increased in symptomatic compared with asymptomatic CPs at both the mRNA level (1.82+/-0.19[SE] versus 1.25+/-0.15, P=0.012) and the protein level (1.04+/-0.23 versus 0.46+/-0.14, P=0.043). Adipophilin colocalized with macrophage foam cells, extravasated red blood cells (P<0.0001), and cholesterol crystals (P<0.0001), and its expression associated with macroscopic ulceration of CP (P<0.0001).
Intraplaque hemorrhages may contribute to intracellular lipid accumulation and consequent adipophilin expression. Because adipophilin blocks cholesterol efflux from lipid-laden cells, they may die and develop a necrotic lipid core, thereby destabilizing the plaque.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>17446422</pmid><doi>10.1161/STROKEAHA.106.478867</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; American Heart Association Journals; Journals@Ovid Complete; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aged Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Cardiology. Vascular system Carotid Artery Diseases - genetics Carotid Artery Diseases - metabolism Carotid Artery Diseases - pathology Carotid Artery, Internal - metabolism Carotid Artery, Internal - pathology Cholesterol - analysis Cholesterol - metabolism Crystallization Erythrocytes - metabolism Female Gene Expression Regulation - physiology Humans Male Medical sciences Membrane Proteins Middle Aged Necrosis Neurology Neurosurgery Peptides - genetics Peptides - metabolism Perilipin-2 Skull, brain, vascular surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Vascular diseases and vascular malformations of the nervous system |
| title | Adipophilin expression is increased in symptomatic carotid atherosclerosis : Correlation with red blood cells and cholesterol crystals |
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