Comparison of Spiculogenesis in in Vitro ADCP-primmorph and Explants Culture of Marine Sponge Hymeniacidon perleve with 3-TMOSPU Supplementation
This study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3‐(trimethoxysilyl)propyl]urea (3‐TMOSPU) as silica source in the cultures of archaeocytes‐dominant‐cell‐population (ADCP) primm...
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Veröffentlicht in: | Biotechnology progress 2007-05, Vol.23 (3), p.707-714 |
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description | This study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3‐(trimethoxysilyl)propyl]urea (3‐TMOSPU) as silica source in the cultures of archaeocytes‐dominant‐cell‐population (ADCP) primmorphs and explants of the marine sponge Hymeniacidon perleve. Analysis by Fourier Transform Infrared Spectroscopy (FT‐IR) confirmed that the organic group in 3‐TMOSPU was introduced into silica spicules. By comparing ADCP‐primmorph cultures when supplemented with Na2SiO3, 3‐TMOSPU supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. A decline in silicatein expression quantified by real‐time RT‐PCR was, however, observed during spiculogenesis. The decline was slower for the 3‐TMOSPU group whereas significantly fewer spicules were formed. When sponge papillae explants were cultured, 3‐TMOSPU supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom Nitzschia closterium. By monitoring the detectable Si concentration, it seemed that 3‐TMOSPU was converted by the sponge and its conversion was related to spiculogenesis. Analysis of spicule dimensional changes indicated that the inhibition of spiculogenesis by 3‐TMOSPU supplementation was less in ADCP‐primmorphs culture due to lower 3‐TMOSPU/detectable Si ratio in the media. |
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Analysis by Fourier Transform Infrared Spectroscopy (FT‐IR) confirmed that the organic group in 3‐TMOSPU was introduced into silica spicules. By comparing ADCP‐primmorph cultures when supplemented with Na2SiO3, 3‐TMOSPU supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. A decline in silicatein expression quantified by real‐time RT‐PCR was, however, observed during spiculogenesis. The decline was slower for the 3‐TMOSPU group whereas significantly fewer spicules were formed. When sponge papillae explants were cultured, 3‐TMOSPU supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom Nitzschia closterium. By monitoring the detectable Si concentration, it seemed that 3‐TMOSPU was converted by the sponge and its conversion was related to spiculogenesis. Analysis of spicule dimensional changes indicated that the inhibition of spiculogenesis by 3‐TMOSPU supplementation was less in ADCP‐primmorphs culture due to lower 3‐TMOSPU/detectable Si ratio in the media.</description><identifier>ISSN: 8756-7938</identifier><identifier>EISSN: 1520-6033</identifier><identifier>DOI: 10.1021/bp0603659</identifier><identifier>PMID: 17461548</identifier><identifier>CODEN: BIPRET</identifier><language>eng</language><publisher>USA: American Chemical Society</publisher><subject>Animals ; Bacillariophyceae ; Biological and medical sciences ; Biomass ; Biotechnology ; Cathepsins - genetics ; Cathepsins - metabolism ; Cell Culture Techniques - methods ; Cell Differentiation ; Fundamental and applied biological sciences. Psychology ; Hymeniacidon perleve ; Nitzschia closterium ; Porifera - cytology ; Porifera - growth & development ; Porifera - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Silicon - administration & dosage ; Silicon - chemistry ; Silicon - metabolism ; Spectroscopy, Fourier Transform Infrared</subject><ispartof>Biotechnology progress, 2007-05, Vol.23 (3), p.707-714</ispartof><rights>Copyright © 2007 American Institute of Chemical Engineers (AIChE)</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4249-d6bfe7e5164700ebbb35401dfa5d62cdf61e93253bec5c73dc1b8e49e098eea03</citedby><cites>FETCH-LOGICAL-c4249-d6bfe7e5164700ebbb35401dfa5d62cdf61e93253bec5c73dc1b8e49e098eea03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1021%2Fbp0603659$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1021%2Fbp0603659$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18841688$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17461548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cao, Xupeng</creatorcontrib><creatorcontrib>Yu, Xingju</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><title>Comparison of Spiculogenesis in in Vitro ADCP-primmorph and Explants Culture of Marine Sponge Hymeniacidon perleve with 3-TMOSPU Supplementation</title><title>Biotechnology progress</title><addtitle>Biotechnol Progress</addtitle><description>This study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3‐(trimethoxysilyl)propyl]urea (3‐TMOSPU) as silica source in the cultures of archaeocytes‐dominant‐cell‐population (ADCP) primmorphs and explants of the marine sponge Hymeniacidon perleve. Analysis by Fourier Transform Infrared Spectroscopy (FT‐IR) confirmed that the organic group in 3‐TMOSPU was introduced into silica spicules. By comparing ADCP‐primmorph cultures when supplemented with Na2SiO3, 3‐TMOSPU supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. A decline in silicatein expression quantified by real‐time RT‐PCR was, however, observed during spiculogenesis. The decline was slower for the 3‐TMOSPU group whereas significantly fewer spicules were formed. When sponge papillae explants were cultured, 3‐TMOSPU supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom Nitzschia closterium. By monitoring the detectable Si concentration, it seemed that 3‐TMOSPU was converted by the sponge and its conversion was related to spiculogenesis. Analysis of spicule dimensional changes indicated that the inhibition of spiculogenesis by 3‐TMOSPU supplementation was less in ADCP‐primmorphs culture due to lower 3‐TMOSPU/detectable Si ratio in the media.</description><subject>Animals</subject><subject>Bacillariophyceae</subject><subject>Biological and medical sciences</subject><subject>Biomass</subject><subject>Biotechnology</subject><subject>Cathepsins - genetics</subject><subject>Cathepsins - metabolism</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hymeniacidon perleve</subject><subject>Nitzschia closterium</subject><subject>Porifera - cytology</subject><subject>Porifera - growth & development</subject><subject>Porifera - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Silicon - administration & dosage</subject><subject>Silicon - chemistry</subject><subject>Silicon - metabolism</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><issn>8756-7938</issn><issn>1520-6033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEokvhwB9AvoDEIWDH8UeObVq2SP1YsVtAXCzHmbSmSRzshHb_BT8Zr3a1PSGkkayRnnlGnjdJXhP8geCMfKwGzDHlrHiSzAjLcBo7-jSZScF4KgoqD5IXIfzEGEvMs-fJARE5JyyXs-RP6bpBextcj1yDloM1U-tuoIdgA7L9pr7a0Tt0dFIu0sHbrnN-uEW6r9Hpw9DqfgyonNpx8rAxXERZD1Hk-htAZ-sOequNraN_AN_Cb0D3drxFNF1dXC0X12g5DUMLERv1aF3_MnnW6DbAq917mFx_Ol2VZ-n51fxzeXSemjzLi7TmVQMCGOG5wBiqqqIsx6RuNKt5ZuqGEyhoxmgFhhlBa0MqCXkBuJAAGtPD5N3WO3j3a4Iwqs4GA238ELgpKIFZPGhG_guSQgiOZRHB91vQeBeCh0ZtrqX9WhGsNjmpfU6RfbOTTlUH9SO5CyYCb3eADka3jde9seGRkzInXG44suXubQvrf29Ux6vFl_3ydDtjwwgP-xnt7xQXVDD17XKufmTf2fySHasT-heghbpb</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Cao, Xupeng</creator><creator>Yu, Xingju</creator><creator>Zhang, Wei</creator><general>American Chemical Society</general><general>American Institute of Chemical Engineers</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070501</creationdate><title>Comparison of Spiculogenesis in in Vitro ADCP-primmorph and Explants Culture of Marine Sponge Hymeniacidon perleve with 3-TMOSPU Supplementation</title><author>Cao, Xupeng ; Yu, Xingju ; Zhang, Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4249-d6bfe7e5164700ebbb35401dfa5d62cdf61e93253bec5c73dc1b8e49e098eea03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Bacillariophyceae</topic><topic>Biological and medical sciences</topic><topic>Biomass</topic><topic>Biotechnology</topic><topic>Cathepsins - genetics</topic><topic>Cathepsins - metabolism</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hymeniacidon perleve</topic><topic>Nitzschia closterium</topic><topic>Porifera - cytology</topic><topic>Porifera - growth & development</topic><topic>Porifera - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Silicon - administration & dosage</topic><topic>Silicon - chemistry</topic><topic>Silicon - metabolism</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cao, Xupeng</creatorcontrib><creatorcontrib>Yu, Xingju</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology progress</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cao, Xupeng</au><au>Yu, Xingju</au><au>Zhang, Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Spiculogenesis in in Vitro ADCP-primmorph and Explants Culture of Marine Sponge Hymeniacidon perleve with 3-TMOSPU Supplementation</atitle><jtitle>Biotechnology progress</jtitle><addtitle>Biotechnol Progress</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>23</volume><issue>3</issue><spage>707</spage><epage>714</epage><pages>707-714</pages><issn>8756-7938</issn><eissn>1520-6033</eissn><coden>BIPRET</coden><abstract>This study aims to test the feasibility of introducing functional chemical groups into biogenic silica spicules by examining the effect of supplementing a silican coupler [3‐(trimethoxysilyl)propyl]urea (3‐TMOSPU) as silica source in the cultures of archaeocytes‐dominant‐cell‐population (ADCP) primmorphs and explants of the marine sponge Hymeniacidon perleve. Analysis by Fourier Transform Infrared Spectroscopy (FT‐IR) confirmed that the organic group in 3‐TMOSPU was introduced into silica spicules. By comparing ADCP‐primmorph cultures when supplemented with Na2SiO3, 3‐TMOSPU supplementation showed no notable effect on the primmorphs development and cell locomotion behaviors. A decline in silicatein expression quantified by real‐time RT‐PCR was, however, observed during spiculogenesis. The decline was slower for the 3‐TMOSPU group whereas significantly fewer spicules were formed. When sponge papillae explants were cultured, 3‐TMOSPU supplementation had no negative effect on sponge growth but inhibited the growth biofouling of the diatom Nitzschia closterium. By monitoring the detectable Si concentration, it seemed that 3‐TMOSPU was converted by the sponge and its conversion was related to spiculogenesis. 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subjects | Animals Bacillariophyceae Biological and medical sciences Biomass Biotechnology Cathepsins - genetics Cathepsins - metabolism Cell Culture Techniques - methods Cell Differentiation Fundamental and applied biological sciences. Psychology Hymeniacidon perleve Nitzschia closterium Porifera - cytology Porifera - growth & development Porifera - metabolism Reverse Transcriptase Polymerase Chain Reaction Silicon - administration & dosage Silicon - chemistry Silicon - metabolism Spectroscopy, Fourier Transform Infrared |
title | Comparison of Spiculogenesis in in Vitro ADCP-primmorph and Explants Culture of Marine Sponge Hymeniacidon perleve with 3-TMOSPU Supplementation |
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