In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy
In this study we coated a new biocompatible, nanostructured titanium alloy, Ti13Nb13Zr, with a thin layer of hydroxyapatite nanocrystals and we investigated the response of human bone‐marrow‐derived mesenchymal cells. The coating was realized using a slightly supersaturated CaP solution, which provo...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2007-07, Vol.82A (1), p.213-221 |
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| creator | Bigi, A. Nicoli-Aldini, N. Bracci, B. Zavan, B. Boanini, E. Sbaiz, F. Panzavolta, S. Zorzato, G. Giardino, R. Facchini, A. Abatangelo, G. Cortivo, R. |
| description | In this study we coated a new biocompatible, nanostructured titanium alloy, Ti13Nb13Zr, with a thin layer of hydroxyapatite nanocrystals and we investigated the response of human bone‐marrow‐derived mesenchymal cells. The coating was realized using a slightly supersaturated CaP solution, which provokes a fast deposition of nanocrystalline hydroxyapatite. A thin layer of deposition is appreciable on the etched Ti13Nb13Zr substrates after just 1.5 h soaking in the CaP solution, and it reaches a thickness of 1–2 μm after 3 h soaking. The coating seems thinner than that deposited on Ti6Al4V, which was examined for comparison, likely because of the different roughness profiles of the two etched alloys, and it is constituted of elongated HA nanocrystals, with a mean length of about 100 nm. Mesenchymal stem cells were seeded onto coated and uncoated Ti alloys and cultured for up to 35 days. Cell morphology, proliferation and differentiation were evaluated. The cells display good adhesion and proliferation on the uncoated substrates, whereas the presence of hydroxyapatite coating slightly reduces cell proliferation and induces differentiation of MSCs towards a phenotypic osteoblastic lineage, in agreement with the increase of the expression of osteopontin, osteonectin and collagen type I, evaluated by means of rt‐PCR. Type I collagen expression is higher in Ti13Nb13Zr MSC culture compared to Ti6Al4V, standing for a more efficient extracellular matrix deposition. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007 |
| doi_str_mv | 10.1002/jbm.a.31132 |
| format | Article |
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The coating was realized using a slightly supersaturated CaP solution, which provokes a fast deposition of nanocrystalline hydroxyapatite. A thin layer of deposition is appreciable on the etched Ti13Nb13Zr substrates after just 1.5 h soaking in the CaP solution, and it reaches a thickness of 1–2 μm after 3 h soaking. The coating seems thinner than that deposited on Ti6Al4V, which was examined for comparison, likely because of the different roughness profiles of the two etched alloys, and it is constituted of elongated HA nanocrystals, with a mean length of about 100 nm. Mesenchymal stem cells were seeded onto coated and uncoated Ti alloys and cultured for up to 35 days. Cell morphology, proliferation and differentiation were evaluated. The cells display good adhesion and proliferation on the uncoated substrates, whereas the presence of hydroxyapatite coating slightly reduces cell proliferation and induces differentiation of MSCs towards a phenotypic osteoblastic lineage, in agreement with the increase of the expression of osteopontin, osteonectin and collagen type I, evaluated by means of rt‐PCR. Type I collagen expression is higher in Ti13Nb13Zr MSC culture compared to Ti6Al4V, standing for a more efficient extracellular matrix deposition. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</description><identifier>ISSN: 1549-3296</identifier><identifier>EISSN: 1552-4965</identifier><identifier>DOI: 10.1002/jbm.a.31132</identifier><identifier>PMID: 17266017</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Alloys - chemistry ; Base Sequence ; Bone Marrow Cells - cytology ; Bone Marrow Cells - metabolism ; Cell Adhesion ; Cell Culture Techniques - methods ; Cell Differentiation ; Cell Proliferation ; Coated Materials, Biocompatible - chemistry ; coating ; Collagen Type I - genetics ; DNA Primers - genetics ; Durapatite - chemistry ; Gene Expression ; Humans ; Materials Testing ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Microscopy, Electron, Scanning ; nanocrystalline hydroxyapatite ; Nanoparticles - chemistry ; ostegenic markers ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Osteonectin - genetics ; Osteopontin - genetics ; proliferation ; Ti alloys ; Titanium - chemistry</subject><ispartof>Journal of biomedical materials research. Part A, 2007-07, Vol.82A (1), p.213-221</ispartof><rights>Copyright © 2007 Wiley Periodicals, Inc.</rights><rights>Copyright 2007 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5012-f03a479b9d88b9d059e4eaed65f70f9be4fea0b8eb3597529da5ca94edcaf2f13</citedby><cites>FETCH-LOGICAL-c5012-f03a479b9d88b9d059e4eaed65f70f9be4fea0b8eb3597529da5ca94edcaf2f13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbm.a.31132$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbm.a.31132$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17266017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bigi, A.</creatorcontrib><creatorcontrib>Nicoli-Aldini, N.</creatorcontrib><creatorcontrib>Bracci, B.</creatorcontrib><creatorcontrib>Zavan, B.</creatorcontrib><creatorcontrib>Boanini, E.</creatorcontrib><creatorcontrib>Sbaiz, F.</creatorcontrib><creatorcontrib>Panzavolta, S.</creatorcontrib><creatorcontrib>Zorzato, G.</creatorcontrib><creatorcontrib>Giardino, R.</creatorcontrib><creatorcontrib>Facchini, A.</creatorcontrib><creatorcontrib>Abatangelo, G.</creatorcontrib><creatorcontrib>Cortivo, R.</creatorcontrib><title>In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy</title><title>Journal of biomedical materials research. Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>In this study we coated a new biocompatible, nanostructured titanium alloy, Ti13Nb13Zr, with a thin layer of hydroxyapatite nanocrystals and we investigated the response of human bone‐marrow‐derived mesenchymal cells. The coating was realized using a slightly supersaturated CaP solution, which provokes a fast deposition of nanocrystalline hydroxyapatite. A thin layer of deposition is appreciable on the etched Ti13Nb13Zr substrates after just 1.5 h soaking in the CaP solution, and it reaches a thickness of 1–2 μm after 3 h soaking. The coating seems thinner than that deposited on Ti6Al4V, which was examined for comparison, likely because of the different roughness profiles of the two etched alloys, and it is constituted of elongated HA nanocrystals, with a mean length of about 100 nm. Mesenchymal stem cells were seeded onto coated and uncoated Ti alloys and cultured for up to 35 days. Cell morphology, proliferation and differentiation were evaluated. The cells display good adhesion and proliferation on the uncoated substrates, whereas the presence of hydroxyapatite coating slightly reduces cell proliferation and induces differentiation of MSCs towards a phenotypic osteoblastic lineage, in agreement with the increase of the expression of osteopontin, osteonectin and collagen type I, evaluated by means of rt‐PCR. Type I collagen expression is higher in Ti13Nb13Zr MSC culture compared to Ti6Al4V, standing for a more efficient extracellular matrix deposition. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</description><subject>Alloys - chemistry</subject><subject>Base Sequence</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>coating</subject><subject>Collagen Type I - genetics</subject><subject>DNA Primers - genetics</subject><subject>Durapatite - chemistry</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Materials Testing</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Microscopy, Electron, Scanning</subject><subject>nanocrystalline hydroxyapatite</subject><subject>Nanoparticles - chemistry</subject><subject>ostegenic markers</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteonectin - genetics</subject><subject>Osteopontin - genetics</subject><subject>proliferation</subject><subject>Ti alloys</subject><subject>Titanium - chemistry</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1v1DAQBmALgegHnLgjn7igLP6I7fWRVrQUSjlQhMTFmjhjNSWJF9uhzb8nyy5wg4vHh2dejV5CnnG24oyJV7fNsIKV5FyKB-SQKyWq2mr1cPuvbSWF1QfkKOfbBWumxGNywI3QmnFzSOBipD-6kiL1U1-mhDQGOmDG0d_MA_TUY99nGscS6Qhj9GnOBfq-G5HezG2K9zNsoHQFKx-hYEuvOy6vGi6_Jrq4OD8hjwL0GZ_u5zH5fPbm-vRtdfnx_OL09WXlFeOiCkxCbWxj2_V6eZiyWCNgq1UwLNgG64DAmjU2UlmjhG1BebA1th6CCFwekxe73E2K3yfMxQ1d3l4PI8YpO8OUVkLp_0Jh18YIqRb4cgd9ijknDG6TugHS7Dhz2-rdUr0D96v6RT_fx07NgO1fu-96AXwH7roe539luXcnH36HVrudLhe8_7MD6ZvTRhrlvlyduxOp5Sf1_sxJ-RPuhp9w</recordid><startdate>200707</startdate><enddate>200707</enddate><creator>Bigi, A.</creator><creator>Nicoli-Aldini, N.</creator><creator>Bracci, B.</creator><creator>Zavan, B.</creator><creator>Boanini, E.</creator><creator>Sbaiz, F.</creator><creator>Panzavolta, S.</creator><creator>Zorzato, G.</creator><creator>Giardino, R.</creator><creator>Facchini, A.</creator><creator>Abatangelo, G.</creator><creator>Cortivo, R.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200707</creationdate><title>In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy</title><author>Bigi, A. ; Nicoli-Aldini, N. ; Bracci, B. ; Zavan, B. ; Boanini, E. ; Sbaiz, F. ; Panzavolta, S. ; Zorzato, G. ; Giardino, R. ; Facchini, A. ; Abatangelo, G. ; Cortivo, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5012-f03a479b9d88b9d059e4eaed65f70f9be4fea0b8eb3597529da5ca94edcaf2f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Alloys - chemistry</topic><topic>Base Sequence</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cell Adhesion</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>coating</topic><topic>Collagen Type I - genetics</topic><topic>DNA Primers - genetics</topic><topic>Durapatite - chemistry</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Materials Testing</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Microscopy, Electron, Scanning</topic><topic>nanocrystalline hydroxyapatite</topic><topic>Nanoparticles - chemistry</topic><topic>ostegenic markers</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - metabolism</topic><topic>Osteonectin - genetics</topic><topic>Osteopontin - genetics</topic><topic>proliferation</topic><topic>Ti alloys</topic><topic>Titanium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bigi, A.</creatorcontrib><creatorcontrib>Nicoli-Aldini, N.</creatorcontrib><creatorcontrib>Bracci, B.</creatorcontrib><creatorcontrib>Zavan, B.</creatorcontrib><creatorcontrib>Boanini, E.</creatorcontrib><creatorcontrib>Sbaiz, F.</creatorcontrib><creatorcontrib>Panzavolta, S.</creatorcontrib><creatorcontrib>Zorzato, G.</creatorcontrib><creatorcontrib>Giardino, R.</creatorcontrib><creatorcontrib>Facchini, A.</creatorcontrib><creatorcontrib>Abatangelo, G.</creatorcontrib><creatorcontrib>Cortivo, R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bigi, A.</au><au>Nicoli-Aldini, N.</au><au>Bracci, B.</au><au>Zavan, B.</au><au>Boanini, E.</au><au>Sbaiz, F.</au><au>Panzavolta, S.</au><au>Zorzato, G.</au><au>Giardino, R.</au><au>Facchini, A.</au><au>Abatangelo, G.</au><au>Cortivo, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2007-07</date><risdate>2007</risdate><volume>82A</volume><issue>1</issue><spage>213</spage><epage>221</epage><pages>213-221</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>In this study we coated a new biocompatible, nanostructured titanium alloy, Ti13Nb13Zr, with a thin layer of hydroxyapatite nanocrystals and we investigated the response of human bone‐marrow‐derived mesenchymal cells. The coating was realized using a slightly supersaturated CaP solution, which provokes a fast deposition of nanocrystalline hydroxyapatite. A thin layer of deposition is appreciable on the etched Ti13Nb13Zr substrates after just 1.5 h soaking in the CaP solution, and it reaches a thickness of 1–2 μm after 3 h soaking. The coating seems thinner than that deposited on Ti6Al4V, which was examined for comparison, likely because of the different roughness profiles of the two etched alloys, and it is constituted of elongated HA nanocrystals, with a mean length of about 100 nm. Mesenchymal stem cells were seeded onto coated and uncoated Ti alloys and cultured for up to 35 days. Cell morphology, proliferation and differentiation were evaluated. The cells display good adhesion and proliferation on the uncoated substrates, whereas the presence of hydroxyapatite coating slightly reduces cell proliferation and induces differentiation of MSCs towards a phenotypic osteoblastic lineage, in agreement with the increase of the expression of osteopontin, osteonectin and collagen type I, evaluated by means of rt‐PCR. Type I collagen expression is higher in Ti13Nb13Zr MSC culture compared to Ti6Al4V, standing for a more efficient extracellular matrix deposition. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17266017</pmid><doi>10.1002/jbm.a.31132</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alloys - chemistry Base Sequence Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Cell Adhesion Cell Culture Techniques - methods Cell Differentiation Cell Proliferation Coated Materials, Biocompatible - chemistry coating Collagen Type I - genetics DNA Primers - genetics Durapatite - chemistry Gene Expression Humans Materials Testing Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Microscopy, Electron, Scanning nanocrystalline hydroxyapatite Nanoparticles - chemistry ostegenic markers Osteoblasts - cytology Osteoblasts - metabolism Osteonectin - genetics Osteopontin - genetics proliferation Ti alloys Titanium - chemistry |
| title | In vitro culture of mesenchymal cells onto nanocrystalline hydroxyapatite-coated Ti13Nb13Zr alloy |
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