Cutting Edge: Restoration of the Ability to Generate CTL in Mice Immune to Adenovirus by Delivery of Virus in a Collagen-Based Matrix
Viruses are commonly used for the delivery of genes coding for tumor-associated Ags to elicit tumor-specific immune responses. The success of viral vectors has been limited in preclinical and clinical trials in part because of antiviral immunity. We investigated the ability of a collagen-based matri...
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| Veröffentlicht in: | The Journal of immunology (1950) 2001-01, Vol.166 (2), p.731-735 |
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| container_title | The Journal of immunology (1950) |
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| creator | Siemens, D. Robert Elzey, Bennett D Lubaroff, David M Bohlken, Caitlin Jensen, Robert J Swanson, Axel Karl Ratliff, Timothy L |
| description | Viruses are commonly used for the delivery of genes coding for tumor-associated Ags to elicit tumor-specific immune responses. The success of viral vectors has been limited in preclinical and clinical trials in part because of antiviral immunity. We investigated the ability of a collagen-based matrix (Gelfoam; Pharmacia and Upjohn, Kalamazoo, MI) to improve CTL activation by recombinant adenovirus. The data show that coinjection of Gelfoam with type 5 adenovirus recombinant for prostate-specific Ag (Ad5-PSA) enhanced CTL activation. Ad5-PSA priming in Gelfoam also abrogated the inhibitory effects of adenoviral immunity on CTL activation in mice naive to PSA but immune to adenovirus. Finally, Gelfoam enhanced immunization in a self-Ag model using type 5 adenovirus recombinant for membrane-bound OVA (Ad5-mOVA) in rat insulin promoter (RIP)-mOVA-transgenic mice. Thus, Gelfoam enhances CTL activation by recombinant viral vectors in a setting where preformed Ab to the virus is present and also in a tolerant self-Ag model. |
| doi_str_mv | 10.4049/jimmunol.166.2.731 |
| format | Article |
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Robert ; Elzey, Bennett D ; Lubaroff, David M ; Bohlken, Caitlin ; Jensen, Robert J ; Swanson, Axel Karl ; Ratliff, Timothy L</creator><creatorcontrib>Siemens, D. Robert ; Elzey, Bennett D ; Lubaroff, David M ; Bohlken, Caitlin ; Jensen, Robert J ; Swanson, Axel Karl ; Ratliff, Timothy L</creatorcontrib><description>Viruses are commonly used for the delivery of genes coding for tumor-associated Ags to elicit tumor-specific immune responses. The success of viral vectors has been limited in preclinical and clinical trials in part because of antiviral immunity. We investigated the ability of a collagen-based matrix (Gelfoam; Pharmacia and Upjohn, Kalamazoo, MI) to improve CTL activation by recombinant adenovirus. The data show that coinjection of Gelfoam with type 5 adenovirus recombinant for prostate-specific Ag (Ad5-PSA) enhanced CTL activation. Ad5-PSA priming in Gelfoam also abrogated the inhibitory effects of adenoviral immunity on CTL activation in mice naive to PSA but immune to adenovirus. Finally, Gelfoam enhanced immunization in a self-Ag model using type 5 adenovirus recombinant for membrane-bound OVA (Ad5-mOVA) in rat insulin promoter (RIP)-mOVA-transgenic mice. 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Robert</creatorcontrib><creatorcontrib>Elzey, Bennett D</creatorcontrib><creatorcontrib>Lubaroff, David M</creatorcontrib><creatorcontrib>Bohlken, Caitlin</creatorcontrib><creatorcontrib>Jensen, Robert J</creatorcontrib><creatorcontrib>Swanson, Axel Karl</creatorcontrib><creatorcontrib>Ratliff, Timothy L</creatorcontrib><title>Cutting Edge: Restoration of the Ability to Generate CTL in Mice Immune to Adenovirus by Delivery of Virus in a Collagen-Based Matrix</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Viruses are commonly used for the delivery of genes coding for tumor-associated Ags to elicit tumor-specific immune responses. The success of viral vectors has been limited in preclinical and clinical trials in part because of antiviral immunity. We investigated the ability of a collagen-based matrix (Gelfoam; Pharmacia and Upjohn, Kalamazoo, MI) to improve CTL activation by recombinant adenovirus. The data show that coinjection of Gelfoam with type 5 adenovirus recombinant for prostate-specific Ag (Ad5-PSA) enhanced CTL activation. Ad5-PSA priming in Gelfoam also abrogated the inhibitory effects of adenoviral immunity on CTL activation in mice naive to PSA but immune to adenovirus. Finally, Gelfoam enhanced immunization in a self-Ag model using type 5 adenovirus recombinant for membrane-bound OVA (Ad5-mOVA) in rat insulin promoter (RIP)-mOVA-transgenic mice. Thus, Gelfoam enhances CTL activation by recombinant viral vectors in a setting where preformed Ab to the virus is present and also in a tolerant self-Ag model.</description><subject>Adenoviruses, Human - genetics</subject><subject>Adenoviruses, Human - immunology</subject><subject>Adjuvants, Immunologic - administration & dosage</subject><subject>Adjuvants, Immunologic - genetics</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Collagen - administration & dosage</subject><subject>Collagen - immunology</subject><subject>Cytotoxicity, Immunologic - genetics</subject><subject>Gelatin Sponge, Absorbable - administration & dosage</subject><subject>Genetic Vectors - administration & dosage</subject><subject>Genetic Vectors - immunology</subject><subject>Humans</subject><subject>Immunization Schedule</subject><subject>Lymphocyte Activation - genetics</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred DBA</subject><subject>Mice, Transgenic</subject><subject>Prostate-Specific Antigen - administration & dosage</subject><subject>Prostate-Specific Antigen - biosynthesis</subject><subject>Prostate-Specific Antigen - genetics</subject><subject>Prostate-Specific Antigen - immunology</subject><subject>Rats</subject><subject>Swine</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>T-Lymphocytes, Cytotoxic - virology</subject><subject>Tumor Cells, Cultured</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFu2zAMhoVhxZq1e4EdBp12c0ZJtuTslnpdVyBFgaLdVZBtOlFhW60kN8sD7L2nrClyIkD-_0_yI-Qzg3kO-eLbox2GaXT9nEk553Ml2DsyY0UBmZQg35MZAOcZU1Kdko8hPAKABJ5_IKeMsbyQuZiRv9UUox3X9LJd43d6hyE6b6J1I3UdjRuky9r2Nu5odPQKR0xDpNX9itqR3tgG6fX-CNyPly2O7sX6KdB6R39gb1_Q7_Y5v_83k8PQyvW9WeOYXZiALb0x0ds_5-SkM33AT4d6Rh5-Xt5Xv7LV7dV1tVxljVB5zGpe1rCArhN13qBQnC2YqaFQqsCyhUaiMSUXqikFtLItWgNNYcpOMCZq1glxRr6-5j559zylX_VgQ4PpohHdFLSCRIUXKgn5q7DxLgSPnX7ydjB-pxnoPXz9Bl8n-JrrBD-ZvhzSp3rA9mg50D6u39j1Zms96jCYvk9yprfb7THpH4egkBc</recordid><startdate>20010115</startdate><enddate>20010115</enddate><creator>Siemens, D. Robert</creator><creator>Elzey, Bennett D</creator><creator>Lubaroff, David M</creator><creator>Bohlken, Caitlin</creator><creator>Jensen, Robert J</creator><creator>Swanson, Axel Karl</creator><creator>Ratliff, Timothy L</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20010115</creationdate><title>Cutting Edge: Restoration of the Ability to Generate CTL in Mice Immune to Adenovirus by Delivery of Virus in a Collagen-Based Matrix</title><author>Siemens, D. Robert ; Elzey, Bennett D ; Lubaroff, David M ; Bohlken, Caitlin ; Jensen, Robert J ; Swanson, Axel Karl ; Ratliff, Timothy L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-b28b090ff3b4ce372191ab05775e8d0c6eaa8237c830d6d5da0c5a8f3113b1f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adenoviruses, Human - genetics</topic><topic>Adenoviruses, Human - immunology</topic><topic>Adjuvants, Immunologic - administration & dosage</topic><topic>Adjuvants, Immunologic - genetics</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Collagen - administration & dosage</topic><topic>Collagen - immunology</topic><topic>Cytotoxicity, Immunologic - genetics</topic><topic>Gelatin Sponge, Absorbable - administration & dosage</topic><topic>Genetic Vectors - administration & dosage</topic><topic>Genetic Vectors - immunology</topic><topic>Humans</topic><topic>Immunization Schedule</topic><topic>Lymphocyte Activation - genetics</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred DBA</topic><topic>Mice, Transgenic</topic><topic>Prostate-Specific Antigen - administration & dosage</topic><topic>Prostate-Specific Antigen - biosynthesis</topic><topic>Prostate-Specific Antigen - genetics</topic><topic>Prostate-Specific Antigen - immunology</topic><topic>Rats</topic><topic>Swine</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>T-Lymphocytes, Cytotoxic - virology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Siemens, D. Robert</creatorcontrib><creatorcontrib>Elzey, Bennett D</creatorcontrib><creatorcontrib>Lubaroff, David M</creatorcontrib><creatorcontrib>Bohlken, Caitlin</creatorcontrib><creatorcontrib>Jensen, Robert J</creatorcontrib><creatorcontrib>Swanson, Axel Karl</creatorcontrib><creatorcontrib>Ratliff, Timothy L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Siemens, D. Robert</au><au>Elzey, Bennett D</au><au>Lubaroff, David M</au><au>Bohlken, Caitlin</au><au>Jensen, Robert J</au><au>Swanson, Axel Karl</au><au>Ratliff, Timothy L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cutting Edge: Restoration of the Ability to Generate CTL in Mice Immune to Adenovirus by Delivery of Virus in a Collagen-Based Matrix</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2001-01-15</date><risdate>2001</risdate><volume>166</volume><issue>2</issue><spage>731</spage><epage>735</epage><pages>731-735</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Viruses are commonly used for the delivery of genes coding for tumor-associated Ags to elicit tumor-specific immune responses. The success of viral vectors has been limited in preclinical and clinical trials in part because of antiviral immunity. 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| subjects | Adenoviruses, Human - genetics Adenoviruses, Human - immunology Adjuvants, Immunologic - administration & dosage Adjuvants, Immunologic - genetics Animals Cell Line Collagen - administration & dosage Collagen - immunology Cytotoxicity, Immunologic - genetics Gelatin Sponge, Absorbable - administration & dosage Genetic Vectors - administration & dosage Genetic Vectors - immunology Humans Immunization Schedule Lymphocyte Activation - genetics Mice Mice, Inbred BALB C Mice, Inbred DBA Mice, Transgenic Prostate-Specific Antigen - administration & dosage Prostate-Specific Antigen - biosynthesis Prostate-Specific Antigen - genetics Prostate-Specific Antigen - immunology Rats Swine T-Lymphocytes, Cytotoxic - immunology T-Lymphocytes, Cytotoxic - virology Tumor Cells, Cultured |
| title | Cutting Edge: Restoration of the Ability to Generate CTL in Mice Immune to Adenovirus by Delivery of Virus in a Collagen-Based Matrix |
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