Wide-bore monolithic column for electrochromatography
A new wide‐bore electrophoresis (WE) system adopting an inner cooling device was set up to perform electrochromatography. In this system, a quartz tube of 1.2 mm inner diameter was used as the separation channel. The Joule heat generated during electrophoresis was removed timely through the outer su...
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| Veröffentlicht in: | Electrophoresis 2007-06, Vol.28 (11), p.1674-1680 |
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| container_title | Electrophoresis |
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| creator | Yuan, Ruijuan Ding, Guosheng Guo, Yugao Liu, Danning Bao, James J. |
| description | A new wide‐bore electrophoresis (WE) system adopting an inner cooling device was set up to perform electrochromatography. In this system, a quartz tube of 1.2 mm inner diameter was used as the separation channel. The Joule heat generated during electrophoresis was removed timely through the outer surface of the quartz tube and a cooling capillary inserted into the quartz tube. A proper coolant passed through the cooling capillary to further improve the cooling efficiency. In the primary research, a polyacrylamide monolithic column was successfully prepared in this quartz tube. Then it was evaluated in the electrochromatographic mode. An electric field strength as high as 625 V/cm can be applied to this system without obvious deviation of the current from the linear curve of the Ohm plot. Sample volume as high as 1 μL was injected into the WE system and reasonable efficiency was obtained for separation of the test compounds. |
| doi_str_mv | 10.1002/elps.200600541 |
| format | Article |
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Sample volume as high as 1 μL was injected into the WE system and reasonable efficiency was obtained for separation of the test compounds.</description><subject>Benzenesulfonates - isolation & purification</subject><subject>Capillary Electrochromatography - instrumentation</subject><subject>Electrochromatography</subject><subject>Electroosmosis</subject><subject>Food Coloring Agents - isolation & purification</subject><subject>Monolithic column</subject><subject>Reproducibility of Results</subject><subject>Rheology</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkDtPwzAYRS0EgvJYGVEntpTPb3tEiKfKqwXBZjmOQwNJXexU0H9PUavC1uku557hIHSIoYcByImvJ6lHAAQAZ3gDdTAnJCNC0U3UASxpBoryHbSb0jsAMM3YNtrBkikAqjuIv1SFz_IQfbcJ41BX7ahyXRfqaTPuliF2fe1dG4MbxdDYNrxFOxnN9tFWaevkD5a7h54vzp_OrrL-_eX12Wk_cwwTnKlCYV2UllnnQAF1QAshiS2tpl6DLawCD5ILoinHXFKdC81UXuKcaWot3UPHC-8khs-pT61pquR8XduxD9NkJHCBCci1IAVQQmA-B3sL0MWQUvSlmcSqsXFmMJjfoua3qFkVnR-OluZp3vjiD18mnAN6AXxVtZ-t0Znz_sPwvzxbfKvU-u_V18YPIySV3LzcXZrhzWDwOrh9NEP6A0x-kWQ</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Yuan, Ruijuan</creator><creator>Ding, Guosheng</creator><creator>Guo, Yugao</creator><creator>Liu, Danning</creator><creator>Bao, James J.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20070601</creationdate><title>Wide-bore monolithic column for electrochromatography</title><author>Yuan, Ruijuan ; Ding, Guosheng ; Guo, Yugao ; Liu, Danning ; Bao, James J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4121-8d819dfa4acc0803c03d672afa93e90ada80e0756293515739b6948bf1b493aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Benzenesulfonates - isolation & purification</topic><topic>Capillary Electrochromatography - instrumentation</topic><topic>Electrochromatography</topic><topic>Electroosmosis</topic><topic>Food Coloring Agents - isolation & purification</topic><topic>Monolithic column</topic><topic>Reproducibility of Results</topic><topic>Rheology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yuan, Ruijuan</creatorcontrib><creatorcontrib>Ding, Guosheng</creatorcontrib><creatorcontrib>Guo, Yugao</creatorcontrib><creatorcontrib>Liu, Danning</creatorcontrib><creatorcontrib>Bao, James J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yuan, Ruijuan</au><au>Ding, Guosheng</au><au>Guo, Yugao</au><au>Liu, Danning</au><au>Bao, James J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wide-bore monolithic column for electrochromatography</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>28</volume><issue>11</issue><spage>1674</spage><epage>1680</epage><pages>1674-1680</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>A new wide‐bore electrophoresis (WE) system adopting an inner cooling device was set up to perform electrochromatography. 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| identifier | ISSN: 0173-0835 |
| ispartof | Electrophoresis, 2007-06, Vol.28 (11), p.1674-1680 |
| issn | 0173-0835 1522-2683 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Benzenesulfonates - isolation & purification Capillary Electrochromatography - instrumentation Electrochromatography Electroosmosis Food Coloring Agents - isolation & purification Monolithic column Reproducibility of Results Rheology |
| title | Wide-bore monolithic column for electrochromatography |
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