Effects of Crowding by Mono-, Di-, and Tetrasaccharides on Cytochrome c−Cytochrome c Peroxidase Binding:  Comparing Experiment to Theory

In cells, protein−protein interactions occur in an environment that is crowded with other molecules, but in vitro studies are almost exclusively performed in dilute solution. To gain information about the effects of crowding on protein complex formation, we used isothermal titration calorimetry to m...

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