Use of a pH-sensitive fluorescent probe for measuring intracellular pH of Caco-2 cells

This paper describes the application of a pH-sensitive fluorescent probe [2′,7′-bis(2-carboxylethyl)-5(6)-carboxyfluorescein or BCECF] to measure intracellular pH (pH i) changes in Caco-2 cells. As a function of BCECF's ionization, the fluorescence was monitored at λ ex = 440 and 503 nm, and λ...

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