Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production
Engineering of the methylmalonyl-CoA (mmCoA) metabolite node of the Saccharopolyspora erythraea wild-type strain through duplication of the mmCoA mutase (MCM) operon led to a 50% increase in erythromycin production in a high-performance oil-based fermentation medium. The MCM operon was carried on a...
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| Veröffentlicht in: | Metabolic engineering 2007-05, Vol.9 (3), p.293-303 |
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| creator | Reeves, Andrew R. Brikun, Igor A. Cernota, William H. Leach, Benjamin I. Gonzalez, Melissa C. Mark Weber, J. |
| description | Engineering of the methylmalonyl-CoA (mmCoA) metabolite node of the
Saccharopolyspora erythraea wild-type strain through duplication of the mmCoA mutase (MCM) operon led to a 50% increase in erythromycin production in a high-performance oil-based fermentation medium. The MCM operon was carried on a 6.8
kb DNA fragment in a plasmid which was inserted by homologous recombination into the
S. erythraea chromosome. The fragment contained one uncharacterized gene, ORF1; three MCM related genes,
mutA,
mutB,
meaB; and one
gntR-family regulatory gene,
mutR. Additional strains were constructed containing partial duplications of the MCM operon, as well as a knockout of ORF1. None of these strains showed any significant alteration in their erythromycin production profile. The combined results showed that increased erythromycin production only occurred in a strain containing a duplication of the entire MCM operon including
mutR and a predicted stem-loop structure overlapping the 3′ terminus of the
mutR coding sequence. |
| doi_str_mv | 10.1016/j.ymben.2007.02.001 |
| format | Article |
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Saccharopolyspora erythraea wild-type strain through duplication of the mmCoA mutase (MCM) operon led to a 50% increase in erythromycin production in a high-performance oil-based fermentation medium. The MCM operon was carried on a 6.8
kb DNA fragment in a plasmid which was inserted by homologous recombination into the
S. erythraea chromosome. The fragment contained one uncharacterized gene, ORF1; three MCM related genes,
mutA,
mutB,
meaB; and one
gntR-family regulatory gene,
mutR. Additional strains were constructed containing partial duplications of the MCM operon, as well as a knockout of ORF1. None of these strains showed any significant alteration in their erythromycin production profile. The combined results showed that increased erythromycin production only occurred in a strain containing a duplication of the entire MCM operon including
mutR and a predicted stem-loop structure overlapping the 3′ terminus of the
mutR coding sequence.</description><identifier>ISSN: 1096-7176</identifier><identifier>EISSN: 1096-7184</identifier><identifier>DOI: 10.1016/j.ymben.2007.02.001</identifier><identifier>PMID: 17482861</identifier><language>eng</language><publisher>Belgium: Elsevier Inc</publisher><subject>Acyl Coenzyme A - genetics ; Acyl Coenzyme A - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Chromosomes, Bacterial - genetics ; Erythromycin ; Erythromycin - biosynthesis ; Genetic Engineering ; Metabolic engineering ; Methylmalonyl-CoA mutase (MCM) ; Open Reading Frames - genetics ; Precursor ; Saccharopolyspora - genetics ; Saccharopolyspora - metabolism ; Saccharopolyspora erythraea ; Strain improvement</subject><ispartof>Metabolic engineering, 2007-05, Vol.9 (3), p.293-303</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-86299ec1f4c3d1ea8e321555b1b4d10a951fd79edf68258a595597c78dd624543</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ymben.2007.02.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17482861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reeves, Andrew R.</creatorcontrib><creatorcontrib>Brikun, Igor A.</creatorcontrib><creatorcontrib>Cernota, William H.</creatorcontrib><creatorcontrib>Leach, Benjamin I.</creatorcontrib><creatorcontrib>Gonzalez, Melissa C.</creatorcontrib><creatorcontrib>Mark Weber, J.</creatorcontrib><title>Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production</title><title>Metabolic engineering</title><addtitle>Metab Eng</addtitle><description>Engineering of the methylmalonyl-CoA (mmCoA) metabolite node of the
Saccharopolyspora erythraea wild-type strain through duplication of the mmCoA mutase (MCM) operon led to a 50% increase in erythromycin production in a high-performance oil-based fermentation medium. The MCM operon was carried on a 6.8
kb DNA fragment in a plasmid which was inserted by homologous recombination into the
S. erythraea chromosome. The fragment contained one uncharacterized gene, ORF1; three MCM related genes,
mutA,
mutB,
meaB; and one
gntR-family regulatory gene,
mutR. Additional strains were constructed containing partial duplications of the MCM operon, as well as a knockout of ORF1. None of these strains showed any significant alteration in their erythromycin production profile. The combined results showed that increased erythromycin production only occurred in a strain containing a duplication of the entire MCM operon including
mutR and a predicted stem-loop structure overlapping the 3′ terminus of the
mutR coding sequence.</description><subject>Acyl Coenzyme A - genetics</subject><subject>Acyl Coenzyme A - metabolism</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Chromosomes, Bacterial - genetics</subject><subject>Erythromycin</subject><subject>Erythromycin - biosynthesis</subject><subject>Genetic Engineering</subject><subject>Metabolic engineering</subject><subject>Methylmalonyl-CoA mutase (MCM)</subject><subject>Open Reading Frames - genetics</subject><subject>Precursor</subject><subject>Saccharopolyspora - genetics</subject><subject>Saccharopolyspora - metabolism</subject><subject>Saccharopolyspora erythraea</subject><subject>Strain improvement</subject><issn>1096-7176</issn><issn>1096-7184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9L7DAUxYMo_v8EwqMrd625naRNFi5k8KkguFDXIU1unQxtMi_pPOi3f60z6O65upfL75wL5xByBbQACtXNuhj7Bn1RUloXtCwohQNyClRWeQ2CHX7tdXVCzlJaTwBwCcfkBGomSlHBKRnv_YfziNH5jyy02bDCrMdhNXa97oIfu3wZ7uaLbkLnBsx8sDiDr9qYlY5hE7oxbULUGcZxWEWNOmtDzJw3EXVCu7-HfjTOZ5sY7NYMLvgLctTqLuHlfp6T99_3b8vH_Pnl4Wl595wbJuWQi6qUEg20zCwsoBa4KIFz3kDDLFAtObS2lmjbSpRcaC45l7WphbVVyThbnJPrne_0-s8W06B6lwx2nfYYtknVdBKAkD-CIJmQks2Oix1oYkgpYqs20fU6jgqomqtRa_VZjZqrUbRUU_KT6tfeftv0aL81-y4m4HYH4JTGX4dRJePQG7QuohmUDe6_D_4Bqa2jTw</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Reeves, Andrew R.</creator><creator>Brikun, Igor A.</creator><creator>Cernota, William H.</creator><creator>Leach, Benjamin I.</creator><creator>Gonzalez, Melissa C.</creator><creator>Mark Weber, J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070501</creationdate><title>Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production</title><author>Reeves, Andrew R. ; 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Saccharopolyspora erythraea wild-type strain through duplication of the mmCoA mutase (MCM) operon led to a 50% increase in erythromycin production in a high-performance oil-based fermentation medium. The MCM operon was carried on a 6.8
kb DNA fragment in a plasmid which was inserted by homologous recombination into the
S. erythraea chromosome. The fragment contained one uncharacterized gene, ORF1; three MCM related genes,
mutA,
mutB,
meaB; and one
gntR-family regulatory gene,
mutR. Additional strains were constructed containing partial duplications of the MCM operon, as well as a knockout of ORF1. None of these strains showed any significant alteration in their erythromycin production profile. The combined results showed that increased erythromycin production only occurred in a strain containing a duplication of the entire MCM operon including
mutR and a predicted stem-loop structure overlapping the 3′ terminus of the
mutR coding sequence.</abstract><cop>Belgium</cop><pub>Elsevier Inc</pub><pmid>17482861</pmid><doi>10.1016/j.ymben.2007.02.001</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Acyl Coenzyme A - genetics Acyl Coenzyme A - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Chromosomes, Bacterial - genetics Erythromycin Erythromycin - biosynthesis Genetic Engineering Metabolic engineering Methylmalonyl-CoA mutase (MCM) Open Reading Frames - genetics Precursor Saccharopolyspora - genetics Saccharopolyspora - metabolism Saccharopolyspora erythraea Strain improvement |
| title | Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production |
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