Immortalization of bovine germ line stem cells by c-myc and hTERT

The limited life span of bovine germ line stem cells in vitro is one of the obstacles to spermatogenesis analysis, genetic manipulation and generating transgenic animal. The aim of this study is to establish immortalized bovine germ line stem cells by c-myc or hTERT. We constructed pEMY and pETE exp...

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Veröffentlicht in:	Animal reproduction science 2007-08, Vol.100 (3), p.371-378
Hauptverfasser:	Bi, Cong-Ming, Zhang, Shi-Qiang, Zhang, Yong, Peng, Shu-Ying, Wang, Li, An, Zhi-Xing, Qi, Aladaer, Lv, Ning
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bovine c-myc cattle Cattle - genetics Cell Line cell lines Cell Proliferation clones cloning (cells) gene expression Gene Expression Regulation Genetic Engineering Germ Cells - cytology Germ Cells - metabolism germplasm conservation Immortalization Immunohistochemistry in vitro studies molecular genetics protein synthesis Proto-Oncogene Proteins c-myc - genetics reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA, Messenger - metabolism spermatocytes spermatogonia Spermatogonial stem cell spermatozoa stem cells telomerase Telomerase - genetics Telomerase - metabolism Telomerase reverse transcriptase (TERT) Time Factors transgenes transgenic animals
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