Effects of oncostatin M on secretion of vascular endothelial growth factor and reconstruction of liver-like structure by fetal liver cells in monolayer and three-dimensional cultures
Vascular endothelial growth factor (VEGF) is crucial for the development and regeneration of the liver. However, there have been no reports about VEGF secretion by cultured fetal liver cells (FLCs). In the present study, the effects of oncostatin M (OSM), which strongly stimulates the growth and alb...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2007-07, Vol.82A (1), p.73-79 |
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| creator | Ehashi, Tomo Koyama, Toshie Ookawa, Keiko Ohshima, Norio Miyoshi, Hirotoshi |
| description | Vascular endothelial growth factor (VEGF) is crucial for the development and regeneration of the liver. However, there have been no reports about VEGF secretion by cultured fetal liver cells (FLCs). In the present study, the effects of oncostatin M (OSM), which strongly stimulates the growth and albumin secretion of FLCs, on VEGF secretion and morphological changes of long‐term cultured FLCs were investigated under three‐dimensional (3‐D) and monolayer conditions. The cultured FLCs proliferated well and showed stable secretion of VEGF for up to 1 month under both monolayer and 3‐D culture conditions. The addition of OSM to cultured cells strongly enhanced VEGF secretion. Compared with 3‐D cultures, VEGF secretion per cell was higher in monolayer cultures. After 1 month in culture, the FLCs in 3‐D cultures formed large aggregates like liver tissue, and FLCs also formed colonies and duct‐like structures after several months of culture even under monolayer conditions. In conclusion, OSM stimulated the secretion of VEGF by cultured FLCs, which seemed to contribute to the development of a liver‐like structure. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007 |
| doi_str_mv | 10.1002/jbm.a.31027 |
| format | Article |
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However, there have been no reports about VEGF secretion by cultured fetal liver cells (FLCs). In the present study, the effects of oncostatin M (OSM), which strongly stimulates the growth and albumin secretion of FLCs, on VEGF secretion and morphological changes of long‐term cultured FLCs were investigated under three‐dimensional (3‐D) and monolayer conditions. The cultured FLCs proliferated well and showed stable secretion of VEGF for up to 1 month under both monolayer and 3‐D culture conditions. The addition of OSM to cultured cells strongly enhanced VEGF secretion. Compared with 3‐D cultures, VEGF secretion per cell was higher in monolayer cultures. After 1 month in culture, the FLCs in 3‐D cultures formed large aggregates like liver tissue, and FLCs also formed colonies and duct‐like structures after several months of culture even under monolayer conditions. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>Vascular endothelial growth factor (VEGF) is crucial for the development and regeneration of the liver. However, there have been no reports about VEGF secretion by cultured fetal liver cells (FLCs). In the present study, the effects of oncostatin M (OSM), which strongly stimulates the growth and albumin secretion of FLCs, on VEGF secretion and morphological changes of long‐term cultured FLCs were investigated under three‐dimensional (3‐D) and monolayer conditions. The cultured FLCs proliferated well and showed stable secretion of VEGF for up to 1 month under both monolayer and 3‐D culture conditions. The addition of OSM to cultured cells strongly enhanced VEGF secretion. Compared with 3‐D cultures, VEGF secretion per cell was higher in monolayer cultures. After 1 month in culture, the FLCs in 3‐D cultures formed large aggregates like liver tissue, and FLCs also formed colonies and duct‐like structures after several months of culture even under monolayer conditions. In conclusion, OSM stimulated the secretion of VEGF by cultured FLCs, which seemed to contribute to the development of a liver‐like structure. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</description><subject>Animals</subject><subject>bioartificial liver</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Proliferation</subject><subject>Fetus - cytology</subject><subject>hepatocyte</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - physiology</subject><subject>Liver, Artificial</subject><subject>long-term culture</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Oncostatin M - pharmacology</subject><subject>porous scaffold</subject><subject>Tissue Engineering</subject><subject>Vascular Endothelial Growth Factor A - biosynthesis</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoqVw4o584oKy2I4dJ0dayhbUBQ4gJC6WP8Zs2iRubadl_xi_D6fZwg1OHnueeTzSWxTPCV4RjOnrCz2s1KoimIoHxSHhnJasrfnDuWZtWdG2PiiexHiR4Rpz-rg4IILWLanqw-LXqXNgUkTeIT8aH5NK3Yg2-YIimACpy1Vu3qhopl4FBKP1aQt9p3r0I_jbtEVOmeQDUqNFAYwfYwqTuR_suxsIZd9dAlrepwBI75CDlA13XWSg7yPK_w5-9L3awSJL2wBQ2m6AMWZbxvMK83x8Wjxyqo_wbH8eFV_fnX45OSvPP63fn7w5Lw2rmSjBtVpTrBUW2FLlhG5xxcCSptGiYdRaXVfGOs205U5Tx7EiwlaNahkDwquj4uXivQr-eoKY5NDFeVs1gp-iFJhzXnP8X5C2DWGCzeCrBTTBxxjAyavQDSrsJMFyzlPmPKWSd3lm-sVeO-kB7F92H2AGyALcdj3s_uWSH44399Jymeligp9_ZlS4lLWoBJffPq4lPnv7-fvmeC1F9Rsfk7_7</recordid><startdate>200707</startdate><enddate>200707</enddate><creator>Ehashi, Tomo</creator><creator>Koyama, Toshie</creator><creator>Ookawa, Keiko</creator><creator>Ohshima, Norio</creator><creator>Miyoshi, Hirotoshi</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200707</creationdate><title>Effects of oncostatin M on secretion of vascular endothelial growth factor and reconstruction of liver-like structure by fetal liver cells in monolayer and three-dimensional cultures</title><author>Ehashi, Tomo ; Koyama, Toshie ; Ookawa, Keiko ; Ohshima, Norio ; Miyoshi, Hirotoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4647-ef9bb20ba070d2af7b9034ed188b7842ddb63cdfb4bd5fb2f50a17d38a944e153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>bioartificial liver</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Proliferation</topic><topic>Fetus - cytology</topic><topic>hepatocyte</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - physiology</topic><topic>Liver, Artificial</topic><topic>long-term culture</topic><topic>Mice</topic><topic>Microscopy, Electron, Scanning</topic><topic>Oncostatin M - pharmacology</topic><topic>porous scaffold</topic><topic>Tissue Engineering</topic><topic>Vascular Endothelial Growth Factor A - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ehashi, Tomo</creatorcontrib><creatorcontrib>Koyama, Toshie</creatorcontrib><creatorcontrib>Ookawa, Keiko</creatorcontrib><creatorcontrib>Ohshima, Norio</creatorcontrib><creatorcontrib>Miyoshi, Hirotoshi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ehashi, Tomo</au><au>Koyama, Toshie</au><au>Ookawa, Keiko</au><au>Ohshima, Norio</au><au>Miyoshi, Hirotoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of oncostatin M on secretion of vascular endothelial growth factor and reconstruction of liver-like structure by fetal liver cells in monolayer and three-dimensional cultures</atitle><jtitle>Journal of biomedical materials research. Part A</jtitle><addtitle>J. Biomed. Mater. Res</addtitle><date>2007-07</date><risdate>2007</risdate><volume>82A</volume><issue>1</issue><spage>73</spage><epage>79</epage><pages>73-79</pages><issn>1549-3296</issn><eissn>1552-4965</eissn><abstract>Vascular endothelial growth factor (VEGF) is crucial for the development and regeneration of the liver. However, there have been no reports about VEGF secretion by cultured fetal liver cells (FLCs). In the present study, the effects of oncostatin M (OSM), which strongly stimulates the growth and albumin secretion of FLCs, on VEGF secretion and morphological changes of long‐term cultured FLCs were investigated under three‐dimensional (3‐D) and monolayer conditions. The cultured FLCs proliferated well and showed stable secretion of VEGF for up to 1 month under both monolayer and 3‐D culture conditions. The addition of OSM to cultured cells strongly enhanced VEGF secretion. Compared with 3‐D cultures, VEGF secretion per cell was higher in monolayer cultures. After 1 month in culture, the FLCs in 3‐D cultures formed large aggregates like liver tissue, and FLCs also formed colonies and duct‐like structures after several months of culture even under monolayer conditions. In conclusion, OSM stimulated the secretion of VEGF by cultured FLCs, which seemed to contribute to the development of a liver‐like structure. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17269136</pmid><doi>10.1002/jbm.a.31027</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals bioartificial liver Cell Culture Techniques - methods Cell Proliferation Fetus - cytology hepatocyte Hepatocytes - cytology Hepatocytes - drug effects Hepatocytes - physiology Liver, Artificial long-term culture Mice Microscopy, Electron, Scanning Oncostatin M - pharmacology porous scaffold Tissue Engineering Vascular Endothelial Growth Factor A - biosynthesis |
| title | Effects of oncostatin M on secretion of vascular endothelial growth factor and reconstruction of liver-like structure by fetal liver cells in monolayer and three-dimensional cultures |
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