Ultrastructural localization of calcium deposits during in vitro culture of pig oocytes
Calcium deposits were localized using the combined oxalate–pyroantimonate technique in follicle‐enclosed oocytes fixed in situ. These deposits can be observed within vacuoles, mitochondria, and on the surface of yolk granules as well as in the caryoplasm, but are absent from the endoplasmic reticulu...
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| Veröffentlicht in: | Molecular reproduction and development 2001-02, Vol.58 (2), p.196-204 |
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| container_title | Molecular reproduction and development |
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| creator | Petr, Jaroslav Rozinek, Jiří Hruban, Vojtěch Jílek, František Sedmíková, Markéta Vaňourková, Zdeňka Němeček, Zbyněk |
| description | Calcium deposits were localized using the combined oxalate–pyroantimonate technique in follicle‐enclosed oocytes fixed in situ. These deposits can be observed within vacuoles, mitochondria, and on the surface of yolk granules as well as in the caryoplasm, but are absent from the endoplasmic reticulum. Isolation of the oocyte from the follicle resulted in the immediate depletion of these calcium deposits. Replenishment of these deposits started during the first 8 hr of in vitro culture of the oocyte and they were gradually replenished to the levels observed before the liberation of oocytes during in vitro maturation to the stage of metaphase II. Mol. Reprod. Dev. 58:196–204, 2001. © 2001 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/1098-2795(200102)58:2<196::AID-MRD9>3.0.CO;2-J |
| format | Article |
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These deposits can be observed within vacuoles, mitochondria, and on the surface of yolk granules as well as in the caryoplasm, but are absent from the endoplasmic reticulum. Isolation of the oocyte from the follicle resulted in the immediate depletion of these calcium deposits. Replenishment of these deposits started during the first 8 hr of in vitro culture of the oocyte and they were gradually replenished to the levels observed before the liberation of oocytes during in vitro maturation to the stage of metaphase II. Mol. Reprod. Dev. 58:196–204, 2001. © 2001 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/1098-2795(200102)58:2<196::AID-MRD9>3.0.CO;2-J</identifier><identifier>PMID: 11139232</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>New York: John Wiley & Sons, Inc</publisher><subject>Animals ; Biological and medical sciences ; Calcium - analysis ; calcium deposits ; Cell differentiation, maturation, development, hematopoiesis ; Cell physiology ; Cells, Cultured ; Female ; Fundamental and applied biological sciences. Psychology ; in vitro ; maturation ; Molecular and cellular biology ; oocyte ; Oocytes - metabolism ; Oocytes - ultrastructure ; Ovary - cytology ; pig ; Swine, Miniature</subject><ispartof>Molecular reproduction and development, 2001-02, Vol.58 (2), p.196-204</ispartof><rights>Copyright © 2001 Wiley‐Liss, Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4879-3f70339d138c0ce3c302f4d3032f65e9818006e093d68ea3671ffb4f5c7d62d93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1098-2795%28200102%2958%3A2%3C196%3A%3AAID-MRD9%3E3.0.CO%3B2-J$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1098-2795%28200102%2958%3A2%3C196%3A%3AAID-MRD9%3E3.0.CO%3B2-J$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=850535$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11139232$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Petr, Jaroslav</creatorcontrib><creatorcontrib>Rozinek, Jiří</creatorcontrib><creatorcontrib>Hruban, Vojtěch</creatorcontrib><creatorcontrib>Jílek, František</creatorcontrib><creatorcontrib>Sedmíková, Markéta</creatorcontrib><creatorcontrib>Vaňourková, Zdeňka</creatorcontrib><creatorcontrib>Němeček, Zbyněk</creatorcontrib><title>Ultrastructural localization of calcium deposits during in vitro culture of pig oocytes</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>Calcium deposits were localized using the combined oxalate–pyroantimonate technique in follicle‐enclosed oocytes fixed in situ. These deposits can be observed within vacuoles, mitochondria, and on the surface of yolk granules as well as in the caryoplasm, but are absent from the endoplasmic reticulum. Isolation of the oocyte from the follicle resulted in the immediate depletion of these calcium deposits. Replenishment of these deposits started during the first 8 hr of in vitro culture of the oocyte and they were gradually replenished to the levels observed before the liberation of oocytes during in vitro maturation to the stage of metaphase II. Mol. Reprod. Dev. 58:196–204, 2001. © 2001 Wiley‐Liss, Inc.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium - analysis</subject><subject>calcium deposits</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>in vitro</subject><subject>maturation</subject><subject>Molecular and cellular biology</subject><subject>oocyte</subject><subject>Oocytes - metabolism</subject><subject>Oocytes - ultrastructure</subject><subject>Ovary - cytology</subject><subject>pig</subject><subject>Swine, Miniature</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkNFu0zAUhiMEYmPwCigSEoKLlGM7TuyCkKYWxqZtRYhpuzvyHHsypHGxE6A8PQ6pyg03XNm_9Z3fR1-WVQRmBIC-IiBFQWvJX1AAAvQlF3P6hshqPj8-XRYXn5byLZvBbLF6TYuze9nhfuD-eC-hKDm9OcgexfgFAKQU8DA7IIQwSRk9zK6v2j6o2IdB90NQbd56rVr3S_XOd7m3eUraDeu8MRsfXR_zZgiuu8tdl393ffC5Hto0aUZ24-5y7_W2N_Fx9sCqNponu_Mou3r_7vPiQ3G-OjldHJ8XuhS1LJitgTHZECY0aMM0A2rLhgGjtuJGCiIAKgOSNZUwilU1sfa2tFzXTUUbyY6y51PvJvhvg4k9rl3Upm1VZ_wQsQbOOVSQwMsJ1MHHGIzFTXBrFbZIAEfVOJrD0RxOqpGniEk1YlKNo2pkCLhYpeezVPh09_NwuzbN37qd2wQ82wEqJos2qE67uOcEB854olYT9cO1ZvsfS_1jpz85NRZTo4u9-blvVOErVjWrOV5fnuBHAeVNvSzxgv0GceWx3w</recordid><startdate>200102</startdate><enddate>200102</enddate><creator>Petr, Jaroslav</creator><creator>Rozinek, Jiří</creator><creator>Hruban, Vojtěch</creator><creator>Jílek, František</creator><creator>Sedmíková, Markéta</creator><creator>Vaňourková, Zdeňka</creator><creator>Němeček, Zbyněk</creator><general>John Wiley & Sons, Inc</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200102</creationdate><title>Ultrastructural localization of calcium deposits during in vitro culture of pig oocytes</title><author>Petr, Jaroslav ; Rozinek, Jiří ; Hruban, Vojtěch ; Jílek, František ; Sedmíková, Markéta ; Vaňourková, Zdeňka ; Němeček, Zbyněk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4879-3f70339d138c0ce3c302f4d3032f65e9818006e093d68ea3671ffb4f5c7d62d93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium - analysis</topic><topic>calcium deposits</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>in vitro</topic><topic>maturation</topic><topic>Molecular and cellular biology</topic><topic>oocyte</topic><topic>Oocytes - metabolism</topic><topic>Oocytes - ultrastructure</topic><topic>Ovary - cytology</topic><topic>pig</topic><topic>Swine, Miniature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petr, Jaroslav</creatorcontrib><creatorcontrib>Rozinek, Jiří</creatorcontrib><creatorcontrib>Hruban, Vojtěch</creatorcontrib><creatorcontrib>Jílek, František</creatorcontrib><creatorcontrib>Sedmíková, Markéta</creatorcontrib><creatorcontrib>Vaňourková, Zdeňka</creatorcontrib><creatorcontrib>Němeček, Zbyněk</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Petr, Jaroslav</au><au>Rozinek, Jiří</au><au>Hruban, Vojtěch</au><au>Jílek, František</au><au>Sedmíková, Markéta</au><au>Vaňourková, Zdeňka</au><au>Němeček, Zbyněk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural localization of calcium deposits during in vitro culture of pig oocytes</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>2001-02</date><risdate>2001</risdate><volume>58</volume><issue>2</issue><spage>196</spage><epage>204</epage><pages>196-204</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>Calcium deposits were localized using the combined oxalate–pyroantimonate technique in follicle‐enclosed oocytes fixed in situ. These deposits can be observed within vacuoles, mitochondria, and on the surface of yolk granules as well as in the caryoplasm, but are absent from the endoplasmic reticulum. Isolation of the oocyte from the follicle resulted in the immediate depletion of these calcium deposits. Replenishment of these deposits started during the first 8 hr of in vitro culture of the oocyte and they were gradually replenished to the levels observed before the liberation of oocytes during in vitro maturation to the stage of metaphase II. Mol. Reprod. 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| subjects | Animals Biological and medical sciences Calcium - analysis calcium deposits Cell differentiation, maturation, development, hematopoiesis Cell physiology Cells, Cultured Female Fundamental and applied biological sciences. Psychology in vitro maturation Molecular and cellular biology oocyte Oocytes - metabolism Oocytes - ultrastructure Ovary - cytology pig Swine, Miniature |
| title | Ultrastructural localization of calcium deposits during in vitro culture of pig oocytes |
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