Targeted deletion of the osteoclast protein-tyrosine phosphatase (PTP-oc) promoter prevents RANKL-mediated osteoclastic differentiation of RAW264.7 cells

An osteoclastic protein-tyrosine phosphatase, PTP-oc, shares the same gene with a renal PTP, Glepp1. This study demonstrated that targeted deletion of PTP-oc promoter by homologous recombination in RAW264.7 cells completely abolished PTP-oc expression without affecting Glepp1 expression. This strate...

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Veröffentlicht in:	FEBS letters 2007-05, Vol.581 (13), p.2503-2508
Hauptverfasser:	Yang, Jeannie H., Amoui, Mehran, Lau, K.-H. William
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals BAC bacterial artificial chromosome Cell Differentiation Cell Line DMEM Dulbecco's modified Eagle's medium FBS fetal bovine serum GAPDH Gene Deletion glyceraldehyde phosphate dehyrogenase herpes simplex virus thymidine kinase Homologous recombination HSV-TK knockout Membrane Proteins - genetics Mice microphthalmia-associated transcription factor Mitf Osteoclasts Osteoclasts - cytology p PTP-oc phosphatase-deficient Plasmids Polymerase Chain Reaction Promoter Promoter deletion Promoter Regions, Genetic Protein Tyrosine Phosphatases - genetics Protein-tyrosine phosphatase PTP PTP-oc promoter RANK RANK ligand RANKL receptor activator of NFκB Receptor-Like Protein Tyrosine Phosphatases, Class 3 Recombination, Genetic Reverse Transcriptase Polymerase Chain Reaction RT-PCR Sequence Deletion siRNA small interference RNA tartrate-resistant acid phosphatase TRACP
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