Sensitive Nonradioactive Detection of mRNA in Tissue Sections: Novel Application of the Whole-mount In Situ Hybridization Protocol

The relative insensitivity of nonradioactive mRNA detection in tissue sections compared to the sensitive nonradioactive detection of single-copy DNA sequences in chromosome spreads, or of mRNA sequences in whole-mount samples, has remained a puzzling issue. Because of the biological significance of...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 2001-01, Vol.49 (1), p.1-8
Hauptverfasser:	Moorman, Antoon F. M., Houweling, Arjan C., de Boer, Piet A. J., Christoffels, Vincent M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aorta - metabolism Biotin - analogs & derivatives Chick Embryo Embryo, Mammalian Frozen Sections Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans In Situ Hybridization - methods Liver - metabolism Mice Rats Rats, Wistar RNA, Messenger - analysis RNA, Messenger - metabolism Temperature Transcription Factors - genetics Transcription Factors - metabolism Tyramine - analogs & derivatives
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