Isolation and expression of the ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori
We isolated a clone encoding a putative angiotensin-converting enzyme-related gene from the wing disc cDNA library of the silkworm, Bombyx mori (refer to as BmAcer). The predicted open reading frame encoded 648 amino acids with about 50% identities with the Drosophila melanogaster angiotensin-conver...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2001, Vol.31 (1), p.97-103 |
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| container_title | Insect biochemistry and molecular biology |
| container_volume | 31 |
| creator | Quan, Guo-Xing Mita, Kazuei Okano, Kazuhiro Shimada, Toru Nanako Ugajin Xia, Zhao Goto, Noriko Kanke, Eiji Kawasaki, Hideki |
| description | We isolated a clone encoding a putative angiotensin-converting enzyme-related gene from the wing disc cDNA library of the silkworm,
Bombyx mori (refer to as
BmAcer). The predicted open reading frame encoded 648 amino acids with about 50% identities with the
Drosophila melanogaster angiotensin-converting enzyme
Ance and
Acer. Northern analysis identified a 2.2-kilobase mRNA which was abundant in wing discs two days after the beginning of wandering. An accumulation of the transcript was observed approximately 2 h after 20–hydroxyecdysone (20E) exposure in vitro and was blocked slightly by a protein synthetic inhibitor. These data suggest that the transcription of the
BmAcer gene is directly 20E-inducible. |
| doi_str_mv | 10.1016/S0965-1748(00)00112-0 |
| format | Article |
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Bombyx mori (refer to as
BmAcer). The predicted open reading frame encoded 648 amino acids with about 50% identities with the
Drosophila melanogaster angiotensin-converting enzyme
Ance and
Acer. Northern analysis identified a 2.2-kilobase mRNA which was abundant in wing discs two days after the beginning of wandering. An accumulation of the transcript was observed approximately 2 h after 20–hydroxyecdysone (20E) exposure in vitro and was blocked slightly by a protein synthetic inhibitor. These data suggest that the transcription of the
BmAcer gene is directly 20E-inducible.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/S0965-1748(00)00112-0</identifier><identifier>PMID: 11102839</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>20–hydroxyecdysone ; Amino Acid Sequence ; Angiotensin-converting enzyme ; Animals ; Base Sequence ; BmAcer gene ; Bombyx - genetics ; Bombyx mori ; Cloning, Molecular ; DNA, Complementary ; Drosophila Proteins ; Ecdysteroids ; Gene Expression ; Genes, Insect ; Insect Proteins - genetics ; Metalloendopeptidases - genetics ; Metamorphosis ; Molecular Sequence Data ; RNA, Messenger ; Steroids - metabolism ; Wing disc ; Wings, Animal</subject><ispartof>Insect biochemistry and molecular biology, 2001, Vol.31 (1), p.97-103</ispartof><rights>2001 Elsevier Science Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-95ff445db74614fd6c21024cdf6207a87ee12e62534a7880a3c17e2bc5baa3b63</citedby><cites>FETCH-LOGICAL-c392t-95ff445db74614fd6c21024cdf6207a87ee12e62534a7880a3c17e2bc5baa3b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0965-1748(00)00112-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,4022,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11102839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Quan, Guo-Xing</creatorcontrib><creatorcontrib>Mita, Kazuei</creatorcontrib><creatorcontrib>Okano, Kazuhiro</creatorcontrib><creatorcontrib>Shimada, Toru</creatorcontrib><creatorcontrib>Nanako Ugajin</creatorcontrib><creatorcontrib>Xia, Zhao</creatorcontrib><creatorcontrib>Goto, Noriko</creatorcontrib><creatorcontrib>Kanke, Eiji</creatorcontrib><creatorcontrib>Kawasaki, Hideki</creatorcontrib><title>Isolation and expression of the ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>We isolated a clone encoding a putative angiotensin-converting enzyme-related gene from the wing disc cDNA library of the silkworm,
Bombyx mori (refer to as
BmAcer). The predicted open reading frame encoded 648 amino acids with about 50% identities with the
Drosophila melanogaster angiotensin-converting enzyme
Ance and
Acer. Northern analysis identified a 2.2-kilobase mRNA which was abundant in wing discs two days after the beginning of wandering. An accumulation of the transcript was observed approximately 2 h after 20–hydroxyecdysone (20E) exposure in vitro and was blocked slightly by a protein synthetic inhibitor. These data suggest that the transcription of the
BmAcer gene is directly 20E-inducible.</description><subject>20–hydroxyecdysone</subject><subject>Amino Acid Sequence</subject><subject>Angiotensin-converting enzyme</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>BmAcer gene</subject><subject>Bombyx - genetics</subject><subject>Bombyx mori</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Drosophila Proteins</subject><subject>Ecdysteroids</subject><subject>Gene Expression</subject><subject>Genes, Insect</subject><subject>Insect Proteins - genetics</subject><subject>Metalloendopeptidases - genetics</subject><subject>Metamorphosis</subject><subject>Molecular Sequence Data</subject><subject>RNA, Messenger</subject><subject>Steroids - metabolism</subject><subject>Wing disc</subject><subject>Wings, Animal</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxa2Kql1aPgLIJwQH07Hj2MkJQcWfSpU4tJwtx54sRom92NnSRep3J-mu4NjTyJqf33uaR8hLDu84cHVxA62qGdeyeQPwFoBzweCIrHijWwZCwjOy-oeckuel_AQAKWt9Qk455yCaql2Rh6uSBjuFFKmNnuL9JmMpyzP1dPqBFJ3flQlzCp6F6LcudAPO7DqkCWMJkbkU7zBPIa4pxj-7EVnGWRI9XWNEGiL9vex8KK4sqh_T2O3u6ZhyOCfHvR0KvjjMM_L986fby6_s-tuXq8sP18xVrZhYW_f9nNx3Wioue6-cmPNL53slQNtGI3KBStSVtLppwFaOaxSdqztrq05VZ-T1XneT068tlsmMcxocBhsxbYvRUMtKKHgS5FqrmVsU6z3ociolY282OYw27wwHsxRkHgsyy_UNgHksyCwGrw4G225E___XoZEZeL8HcL7HXcBsigsYHfqQ0U3Gp_CExV9ZCqIW</recordid><startdate>2001</startdate><enddate>2001</enddate><creator>Quan, Guo-Xing</creator><creator>Mita, Kazuei</creator><creator>Okano, Kazuhiro</creator><creator>Shimada, Toru</creator><creator>Nanako Ugajin</creator><creator>Xia, Zhao</creator><creator>Goto, Noriko</creator><creator>Kanke, Eiji</creator><creator>Kawasaki, Hideki</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>2001</creationdate><title>Isolation and expression of the ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori</title><author>Quan, Guo-Xing ; Mita, Kazuei ; Okano, Kazuhiro ; Shimada, Toru ; Nanako Ugajin ; Xia, Zhao ; Goto, Noriko ; Kanke, Eiji ; Kawasaki, Hideki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c392t-95ff445db74614fd6c21024cdf6207a87ee12e62534a7880a3c17e2bc5baa3b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>20–hydroxyecdysone</topic><topic>Amino Acid Sequence</topic><topic>Angiotensin-converting enzyme</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>BmAcer gene</topic><topic>Bombyx - genetics</topic><topic>Bombyx mori</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Drosophila Proteins</topic><topic>Ecdysteroids</topic><topic>Gene Expression</topic><topic>Genes, Insect</topic><topic>Insect Proteins - genetics</topic><topic>Metalloendopeptidases - genetics</topic><topic>Metamorphosis</topic><topic>Molecular Sequence Data</topic><topic>RNA, Messenger</topic><topic>Steroids - metabolism</topic><topic>Wing disc</topic><topic>Wings, Animal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Quan, Guo-Xing</creatorcontrib><creatorcontrib>Mita, Kazuei</creatorcontrib><creatorcontrib>Okano, Kazuhiro</creatorcontrib><creatorcontrib>Shimada, Toru</creatorcontrib><creatorcontrib>Nanako Ugajin</creatorcontrib><creatorcontrib>Xia, Zhao</creatorcontrib><creatorcontrib>Goto, Noriko</creatorcontrib><creatorcontrib>Kanke, Eiji</creatorcontrib><creatorcontrib>Kawasaki, Hideki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Quan, Guo-Xing</au><au>Mita, Kazuei</au><au>Okano, Kazuhiro</au><au>Shimada, Toru</au><au>Nanako Ugajin</au><au>Xia, Zhao</au><au>Goto, Noriko</au><au>Kanke, Eiji</au><au>Kawasaki, Hideki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and expression of the ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2001</date><risdate>2001</risdate><volume>31</volume><issue>1</issue><spage>97</spage><epage>103</epage><pages>97-103</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>We isolated a clone encoding a putative angiotensin-converting enzyme-related gene from the wing disc cDNA library of the silkworm,
Bombyx mori (refer to as
BmAcer). The predicted open reading frame encoded 648 amino acids with about 50% identities with the
Drosophila melanogaster angiotensin-converting enzyme
Ance and
Acer. Northern analysis identified a 2.2-kilobase mRNA which was abundant in wing discs two days after the beginning of wandering. An accumulation of the transcript was observed approximately 2 h after 20–hydroxyecdysone (20E) exposure in vitro and was blocked slightly by a protein synthetic inhibitor. These data suggest that the transcription of the
BmAcer gene is directly 20E-inducible.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>11102839</pmid><doi>10.1016/S0965-1748(00)00112-0</doi><tpages>7</tpages></addata></record> |
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| subjects | 20–hydroxyecdysone Amino Acid Sequence Angiotensin-converting enzyme Animals Base Sequence BmAcer gene Bombyx - genetics Bombyx mori Cloning, Molecular DNA, Complementary Drosophila Proteins Ecdysteroids Gene Expression Genes, Insect Insect Proteins - genetics Metalloendopeptidases - genetics Metamorphosis Molecular Sequence Data RNA, Messenger Steroids - metabolism Wing disc Wings, Animal |
| title | Isolation and expression of the ecdysteroid-inducible angiotensin-converting enzyme-related gene in wing discs of Bombyx mori |
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