Identification of an antihypertensive peptide from peptic digest of wakame ( Undaria pinnatifida)
A peptide fraction having activity against angiotensin I-converting enzyme (ACE) was separated from the peptic digest of protein prepared from wakame (Undaria pinnatifida) by ion-exchange chromatographies and gel-filtration. Fractions with high ACE inhibitory activity were combined and further chrom...
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| Veröffentlicht in: | The Journal of nutritional biochemistry 2000-09, Vol.11 (9), p.450-454 |
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| creator | Suetsuna, Kunio Nakano, Takahisa |
| description | A peptide fraction having activity against angiotensin I-converting enzyme (ACE) was separated from the peptic digest of protein prepared from wakame
(Undaria pinnatifida) by ion-exchange chromatographies and gel-filtration. Fractions with high ACE inhibitory activity were combined and further chromatographed on a reverse-phase column to yield four tetrapeptides with ACE inhibitory properties. These tetrapeptides were identified by sequence analysis and fast atom bombardment mass spectrometry as Ala-Ile-Tyr-Lys (IC
50: 213 μM), Tyr-Lys-Tyr-Tyr (64.2 μM), Lys-Phe-Tyr-Gly (90.5 μM), and Tyr-Asn-Lys-Leu (21 μM). Each tetrapeptide was synthesized and its antihypertensive activity was determined after oral administration in spontaneously hypertensive rats. The blood pressure significantly decreased after tetrapeptide ingestion. The present study demonstrated that dietary wakame may have beneficial effects on hypertension. |
| doi_str_mv | 10.1016/S0955-2863(00)00110-8 |
| format | Article |
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(Undaria pinnatifida) by ion-exchange chromatographies and gel-filtration. Fractions with high ACE inhibitory activity were combined and further chromatographed on a reverse-phase column to yield four tetrapeptides with ACE inhibitory properties. These tetrapeptides were identified by sequence analysis and fast atom bombardment mass spectrometry as Ala-Ile-Tyr-Lys (IC
50: 213 μM), Tyr-Lys-Tyr-Tyr (64.2 μM), Lys-Phe-Tyr-Gly (90.5 μM), and Tyr-Asn-Lys-Leu (21 μM). Each tetrapeptide was synthesized and its antihypertensive activity was determined after oral administration in spontaneously hypertensive rats. The blood pressure significantly decreased after tetrapeptide ingestion. The present study demonstrated that dietary wakame may have beneficial effects on hypertension.</description><identifier>ISSN: 0955-2863</identifier><identifier>EISSN: 1873-4847</identifier><identifier>DOI: 10.1016/S0955-2863(00)00110-8</identifier><identifier>PMID: 11091100</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>angiotensin I-converting enzyme ; Antihypertensive agents ; Biological and medical sciences ; Cardiovascular system ; inhibitory activity ; Medical sciences ; Pharmacology. Drug treatments ; spontaneously hypertensive rat ; tetrapeptide from wakame</subject><ispartof>The Journal of nutritional biochemistry, 2000-09, Vol.11 (9), p.450-454</ispartof><rights>2000 Elsevier Science Inc.</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-c82667812f3e038108725b5207869a0b6bcc40455e5913eafa24eb8d0a4a88493</citedby><cites>FETCH-LOGICAL-c455t-c82667812f3e038108725b5207869a0b6bcc40455e5913eafa24eb8d0a4a88493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0955286300001108$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=838518$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11091100$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suetsuna, Kunio</creatorcontrib><creatorcontrib>Nakano, Takahisa</creatorcontrib><title>Identification of an antihypertensive peptide from peptic digest of wakame ( Undaria pinnatifida)</title><title>The Journal of nutritional biochemistry</title><addtitle>J Nutr Biochem</addtitle><description>A peptide fraction having activity against angiotensin I-converting enzyme (ACE) was separated from the peptic digest of protein prepared from wakame
(Undaria pinnatifida) by ion-exchange chromatographies and gel-filtration. Fractions with high ACE inhibitory activity were combined and further chromatographed on a reverse-phase column to yield four tetrapeptides with ACE inhibitory properties. These tetrapeptides were identified by sequence analysis and fast atom bombardment mass spectrometry as Ala-Ile-Tyr-Lys (IC
50: 213 μM), Tyr-Lys-Tyr-Tyr (64.2 μM), Lys-Phe-Tyr-Gly (90.5 μM), and Tyr-Asn-Lys-Leu (21 μM). Each tetrapeptide was synthesized and its antihypertensive activity was determined after oral administration in spontaneously hypertensive rats. The blood pressure significantly decreased after tetrapeptide ingestion. The present study demonstrated that dietary wakame may have beneficial effects on hypertension.</description><subject>angiotensin I-converting enzyme</subject><subject>Antihypertensive agents</subject><subject>Biological and medical sciences</subject><subject>Cardiovascular system</subject><subject>inhibitory activity</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>spontaneously hypertensive rat</subject><subject>tetrapeptide from wakame</subject><issn>0955-2863</issn><issn>1873-4847</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkF1L7DAQhoMoun78BKUgiF5UJ23Splci4hcIXqjXYZpMz4lnm_Yk3RX_va276KWQEALP-87wMHbI4ZwDLy6eoZIyzVSRnwKcAXAOqdpgM67KPBVKlJts9o3ssN0Y3wAgE7LYZjsjXI0XZgwfLPnBNc7g4DqfdE2CfjyD-_vRUxjIR7ekpKd-cJaSJnTt6mMS6_5QHKbEO_7DlpLT5NVbDA6T3nmPU6vFs3221eA80sH63WOvtzcv1_fp49Pdw_XVY2qElENqVFYUpeJZkxPkioMqM1nLDEpVVAh1URsjYERJVjwnbDATVCsLKFApUeV77GTV24fu_2LcTLcuGprP0VO3iLoEKXgJagTlCjShizFQo_vgWgwfmoOe3Oovt3oSpwH0l1s95Y7WAxZ1S_YntZY5AsdrAKPBeRPQGxe_OZUryaeayxVFo4ylo6CjceQNWRfIDNp27pdFPgGC5ZTO</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>Suetsuna, Kunio</creator><creator>Nakano, Takahisa</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000901</creationdate><title>Identification of an antihypertensive peptide from peptic digest of wakame ( Undaria pinnatifida)</title><author>Suetsuna, Kunio ; Nakano, Takahisa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-c82667812f3e038108725b5207869a0b6bcc40455e5913eafa24eb8d0a4a88493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>angiotensin I-converting enzyme</topic><topic>Antihypertensive agents</topic><topic>Biological and medical sciences</topic><topic>Cardiovascular system</topic><topic>inhibitory activity</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>spontaneously hypertensive rat</topic><topic>tetrapeptide from wakame</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suetsuna, Kunio</creatorcontrib><creatorcontrib>Nakano, Takahisa</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutritional biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suetsuna, Kunio</au><au>Nakano, Takahisa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of an antihypertensive peptide from peptic digest of wakame ( Undaria pinnatifida)</atitle><jtitle>The Journal of nutritional biochemistry</jtitle><addtitle>J Nutr Biochem</addtitle><date>2000-09-01</date><risdate>2000</risdate><volume>11</volume><issue>9</issue><spage>450</spage><epage>454</epage><pages>450-454</pages><issn>0955-2863</issn><eissn>1873-4847</eissn><abstract>A peptide fraction having activity against angiotensin I-converting enzyme (ACE) was separated from the peptic digest of protein prepared from wakame
(Undaria pinnatifida) by ion-exchange chromatographies and gel-filtration. Fractions with high ACE inhibitory activity were combined and further chromatographed on a reverse-phase column to yield four tetrapeptides with ACE inhibitory properties. These tetrapeptides were identified by sequence analysis and fast atom bombardment mass spectrometry as Ala-Ile-Tyr-Lys (IC
50: 213 μM), Tyr-Lys-Tyr-Tyr (64.2 μM), Lys-Phe-Tyr-Gly (90.5 μM), and Tyr-Asn-Lys-Leu (21 μM). Each tetrapeptide was synthesized and its antihypertensive activity was determined after oral administration in spontaneously hypertensive rats. The blood pressure significantly decreased after tetrapeptide ingestion. The present study demonstrated that dietary wakame may have beneficial effects on hypertension.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>11091100</pmid><doi>10.1016/S0955-2863(00)00110-8</doi><tpages>5</tpages></addata></record> |
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| subjects | angiotensin I-converting enzyme Antihypertensive agents Biological and medical sciences Cardiovascular system inhibitory activity Medical sciences Pharmacology. Drug treatments spontaneously hypertensive rat tetrapeptide from wakame |
| title | Identification of an antihypertensive peptide from peptic digest of wakame ( Undaria pinnatifida) |
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