Comparison of Immunological Properties of Bone Marrow Stromal Cells and Adipose Tissue-Derived Stem Cells Before and After Osteogenic Differentiation In Vitro
Mesenchymal stem cells (MSCs) can be isolated from various tissues and represent an attractive cell population for tissue-engineering purposes. MSCsfrom bonemarrow(bonemarrowstromal cells [BMSCs]) are negative for immunologically relevant surface markers and inhibit proliferation of allogenic T cell...
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| Veröffentlicht in: | Tissue engineering 2007-01, Vol.13 (1), p.111-121 |
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| creator | Niemeyer, Philipp Kornacker, Martin Mehlhorn, Alexander Seckinger, Anja Vohrer, Jana Schmal, Hagen Kasten, Philip Eckstein, Volker Südkamp, Norbert P. Krause, Ulf |
| description | Mesenchymal stem cells (MSCs) can be isolated from various tissues and represent an attractive cell
population for tissue-engineering purposes. MSCsfrom bonemarrow(bonemarrowstromal cells [BMSCs])
are negative for immunologically relevant surface markers and inhibit proliferation of allogenic T cells
in vitro
. Therefore, BMSCs are said to be available for allogenic cell therapy. Although the immunological
characteristics of BMSCs have been the subject of various investigations, those of stem cells
isolated from adipose tissue (ASCs) have not been adequately described. In addition, the influence of
osteogenic differentiation
in vitro
on the immunological characteristics of BMSCs and ASCs is the subject
of this article.
Before and after osteogenic induction, the influence of BMSCs and ASCs on the proliferative behavior of
resting and activated allogenic peripheral blood mononuclear cells (PBMCs) was studied as a measure of
the immune response (mixed lymphocyte culture). At the same points, the expression of immunologically
relevant surface markers (e.g., major histocompatibility complex (MHC)-I, MHC-II, CD40, CD40L) was
measured, and correlations between the different sets of results were sought.
The pattern of surface antigen expression of BMSCs is the same as that of ASCs. Analogous to BMSCs,
undifferentiated cells isolated from adipose tissue lack expression ofMHC-II; this is not lost in the course of
the osteogenic differentiation process. In co-culture with allogenic PBMCs, both cell types fail to lead to any
significant stimulation, and they both retain these characteristics during the differentiation process. BMSCs
and ASCs suppress proliferation on activated PBMCs before and after osteogenic differentiation.
Our results confirm that MSCs are immune modulating cells. These properties are retained even after
osteogenic induction
in vitro
and seem to be similar in BMSCs and ASCs. Our results suggest that allogenic
transplantation of BMSCs and ASCs would be possible, for example, in the context of tissue engineering. |
| doi_str_mv | 10.1089/ten.2006.0114 |
| format | Article |
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population for tissue-engineering purposes. MSCsfrom bonemarrow(bonemarrowstromal cells [BMSCs])
are negative for immunologically relevant surface markers and inhibit proliferation of allogenic T cells
in vitro
. Therefore, BMSCs are said to be available for allogenic cell therapy. Although the immunological
characteristics of BMSCs have been the subject of various investigations, those of stem cells
isolated from adipose tissue (ASCs) have not been adequately described. In addition, the influence of
osteogenic differentiation
in vitro
on the immunological characteristics of BMSCs and ASCs is the subject
of this article.
Before and after osteogenic induction, the influence of BMSCs and ASCs on the proliferative behavior of
resting and activated allogenic peripheral blood mononuclear cells (PBMCs) was studied as a measure of
the immune response (mixed lymphocyte culture). At the same points, the expression of immunologically
relevant surface markers (e.g., major histocompatibility complex (MHC)-I, MHC-II, CD40, CD40L) was
measured, and correlations between the different sets of results were sought.
The pattern of surface antigen expression of BMSCs is the same as that of ASCs. Analogous to BMSCs,
undifferentiated cells isolated from adipose tissue lack expression ofMHC-II; this is not lost in the course of
the osteogenic differentiation process. In co-culture with allogenic PBMCs, both cell types fail to lead to any
significant stimulation, and they both retain these characteristics during the differentiation process. BMSCs
and ASCs suppress proliferation on activated PBMCs before and after osteogenic differentiation.
Our results confirm that MSCs are immune modulating cells. These properties are retained even after
osteogenic induction
in vitro
and seem to be similar in BMSCs and ASCs. Our results suggest that allogenic
transplantation of BMSCs and ASCs would be possible, for example, in the context of tissue engineering.</description><identifier>ISSN: 1076-3279</identifier><identifier>EISSN: 1557-8690</identifier><identifier>DOI: 10.1089/ten.2006.0114</identifier><identifier>PMID: 17518585</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Adipose Tissue - cytology ; Adipose Tissue - immunology ; Adipose Tissue - metabolism ; Antigens, CD - biosynthesis ; Bone Marrow Cells - cytology ; Bone Marrow Cells - immunology ; Bone Marrow Cells - metabolism ; Cell Communication - immunology ; Cell Differentiation - immunology ; Cells, Cultured ; Histocompatibility Antigens Class I - biosynthesis ; Histocompatibility Antigens Class II - biosynthesis ; Humans ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - metabolism ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - immunology ; Mesenchymal Stromal Cells - metabolism ; Osteogenesis - immunology ; Stromal Cells - cytology ; Stromal Cells - immunology ; Stromal Cells - metabolism ; T-Lymphocytes, Regulatory - cytology ; T-Lymphocytes, Regulatory - immunology ; T-Lymphocytes, Regulatory - metabolism ; Time Factors</subject><ispartof>Tissue engineering, 2007-01, Vol.13 (1), p.111-121</ispartof><rights>2007, Mary Ann Liebert, Inc.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-b4ba8b65be68b1a4ec0ad258670a1e7739bcaf9a5ec3b104f27d02c885c9edb13</citedby><cites>FETCH-LOGICAL-c424t-b4ba8b65be68b1a4ec0ad258670a1e7739bcaf9a5ec3b104f27d02c885c9edb13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.liebertpub.com/doi/epdf/10.1089/ten.2006.0114$$EPDF$$P50$$Gmaryannliebert$$H</linktopdf><linktohtml>$$Uhttps://www.liebertpub.com/doi/full/10.1089/ten.2006.0114$$EHTML$$P50$$Gmaryannliebert$$H</linktohtml><link.rule.ids>314,776,780,3028,21703,27903,27904,55269,55281</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17518585$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niemeyer, Philipp</creatorcontrib><creatorcontrib>Kornacker, Martin</creatorcontrib><creatorcontrib>Mehlhorn, Alexander</creatorcontrib><creatorcontrib>Seckinger, Anja</creatorcontrib><creatorcontrib>Vohrer, Jana</creatorcontrib><creatorcontrib>Schmal, Hagen</creatorcontrib><creatorcontrib>Kasten, Philip</creatorcontrib><creatorcontrib>Eckstein, Volker</creatorcontrib><creatorcontrib>Südkamp, Norbert P.</creatorcontrib><creatorcontrib>Krause, Ulf</creatorcontrib><title>Comparison of Immunological Properties of Bone Marrow Stromal Cells and Adipose Tissue-Derived Stem Cells Before and After Osteogenic Differentiation In Vitro</title><title>Tissue engineering</title><addtitle>Tissue Eng</addtitle><description>Mesenchymal stem cells (MSCs) can be isolated from various tissues and represent an attractive cell
population for tissue-engineering purposes. MSCsfrom bonemarrow(bonemarrowstromal cells [BMSCs])
are negative for immunologically relevant surface markers and inhibit proliferation of allogenic T cells
in vitro
. Therefore, BMSCs are said to be available for allogenic cell therapy. Although the immunological
characteristics of BMSCs have been the subject of various investigations, those of stem cells
isolated from adipose tissue (ASCs) have not been adequately described. In addition, the influence of
osteogenic differentiation
in vitro
on the immunological characteristics of BMSCs and ASCs is the subject
of this article.
Before and after osteogenic induction, the influence of BMSCs and ASCs on the proliferative behavior of
resting and activated allogenic peripheral blood mononuclear cells (PBMCs) was studied as a measure of
the immune response (mixed lymphocyte culture). At the same points, the expression of immunologically
relevant surface markers (e.g., major histocompatibility complex (MHC)-I, MHC-II, CD40, CD40L) was
measured, and correlations between the different sets of results were sought.
The pattern of surface antigen expression of BMSCs is the same as that of ASCs. Analogous to BMSCs,
undifferentiated cells isolated from adipose tissue lack expression ofMHC-II; this is not lost in the course of
the osteogenic differentiation process. In co-culture with allogenic PBMCs, both cell types fail to lead to any
significant stimulation, and they both retain these characteristics during the differentiation process. BMSCs
and ASCs suppress proliferation on activated PBMCs before and after osteogenic differentiation.
Our results confirm that MSCs are immune modulating cells. These properties are retained even after
osteogenic induction
in vitro
and seem to be similar in BMSCs and ASCs. Our results suggest that allogenic
transplantation of BMSCs and ASCs would be possible, for example, in the context of tissue engineering.</description><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - immunology</subject><subject>Adipose Tissue - metabolism</subject><subject>Antigens, CD - biosynthesis</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - immunology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cell Communication - immunology</subject><subject>Cell Differentiation - immunology</subject><subject>Cells, Cultured</subject><subject>Histocompatibility Antigens Class I - biosynthesis</subject><subject>Histocompatibility Antigens Class II - biosynthesis</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - immunology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Osteogenesis - immunology</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - immunology</subject><subject>Stromal Cells - metabolism</subject><subject>T-Lymphocytes, Regulatory - cytology</subject><subject>T-Lymphocytes, Regulatory - immunology</subject><subject>T-Lymphocytes, Regulatory - metabolism</subject><subject>Time Factors</subject><issn>1076-3279</issn><issn>1557-8690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9vFCEYBnDSaGxtPfbacPJiZoWZYYBju_XPJjU1afU6AealoZmBKTAav4yfVSa7qfHkCQK_PLzhQeickg0lQr7P4Dc1Id2GUNoeoRPKGK9EJ8mLsie8q5qay2P0OqVHQghjlL9Cx5QzKphgJ-j3Nkyzii4Fj4PFu2lafBjDgzNqxF9jmCFmB2m9uwoe8BcVY_iJ73IMUxFbGMeElR_w5eDmkADfu5QWqK4huh8wFAjTQV2BDRH22GaI-DZlCA_gncHXzlqI4LNT2ZVRdh5_d-WNM_TSqjHBm8N6ir59_HC__Vzd3H7abS9vKtPWba50q5XQHdPQCU1VC4aooWai40RR4LyR2igrFQPTaEpaW_OB1EYIZiQMmjan6O0-d47haYGU-8klU8ZWHsKSek5YQ5iQ_4VUlm9tZFdgtYcmhpQi2H6OblLxV09JvzbXl-b6tbl-ba74i0PwoicY_upDVQW824P1WHk_OtClnGf4b9wfkaumpw</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Niemeyer, Philipp</creator><creator>Kornacker, Martin</creator><creator>Mehlhorn, Alexander</creator><creator>Seckinger, Anja</creator><creator>Vohrer, Jana</creator><creator>Schmal, Hagen</creator><creator>Kasten, Philip</creator><creator>Eckstein, Volker</creator><creator>Südkamp, Norbert P.</creator><creator>Krause, Ulf</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Comparison of Immunological Properties of Bone Marrow Stromal Cells and Adipose Tissue-Derived Stem Cells Before and After Osteogenic Differentiation In Vitro</title><author>Niemeyer, Philipp ; Kornacker, Martin ; Mehlhorn, Alexander ; Seckinger, Anja ; Vohrer, Jana ; Schmal, Hagen ; Kasten, Philip ; Eckstein, Volker ; Südkamp, Norbert P. ; Krause, Ulf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-b4ba8b65be68b1a4ec0ad258670a1e7739bcaf9a5ec3b104f27d02c885c9edb13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - immunology</topic><topic>Adipose Tissue - metabolism</topic><topic>Antigens, CD - biosynthesis</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - immunology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cell Communication - immunology</topic><topic>Cell Differentiation - immunology</topic><topic>Cells, Cultured</topic><topic>Histocompatibility Antigens Class I - biosynthesis</topic><topic>Histocompatibility Antigens Class II - biosynthesis</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - immunology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Osteogenesis - immunology</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - immunology</topic><topic>Stromal Cells - metabolism</topic><topic>T-Lymphocytes, Regulatory - cytology</topic><topic>T-Lymphocytes, Regulatory - immunology</topic><topic>T-Lymphocytes, Regulatory - metabolism</topic><topic>Time Factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Niemeyer, Philipp</creatorcontrib><creatorcontrib>Kornacker, Martin</creatorcontrib><creatorcontrib>Mehlhorn, Alexander</creatorcontrib><creatorcontrib>Seckinger, Anja</creatorcontrib><creatorcontrib>Vohrer, Jana</creatorcontrib><creatorcontrib>Schmal, Hagen</creatorcontrib><creatorcontrib>Kasten, Philip</creatorcontrib><creatorcontrib>Eckstein, Volker</creatorcontrib><creatorcontrib>Südkamp, Norbert P.</creatorcontrib><creatorcontrib>Krause, Ulf</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tissue engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Niemeyer, Philipp</au><au>Kornacker, Martin</au><au>Mehlhorn, Alexander</au><au>Seckinger, Anja</au><au>Vohrer, Jana</au><au>Schmal, Hagen</au><au>Kasten, Philip</au><au>Eckstein, Volker</au><au>Südkamp, Norbert P.</au><au>Krause, Ulf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of Immunological Properties of Bone Marrow Stromal Cells and Adipose Tissue-Derived Stem Cells Before and After Osteogenic Differentiation In Vitro</atitle><jtitle>Tissue engineering</jtitle><addtitle>Tissue Eng</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>13</volume><issue>1</issue><spage>111</spage><epage>121</epage><pages>111-121</pages><issn>1076-3279</issn><eissn>1557-8690</eissn><abstract>Mesenchymal stem cells (MSCs) can be isolated from various tissues and represent an attractive cell
population for tissue-engineering purposes. MSCsfrom bonemarrow(bonemarrowstromal cells [BMSCs])
are negative for immunologically relevant surface markers and inhibit proliferation of allogenic T cells
in vitro
. Therefore, BMSCs are said to be available for allogenic cell therapy. Although the immunological
characteristics of BMSCs have been the subject of various investigations, those of stem cells
isolated from adipose tissue (ASCs) have not been adequately described. In addition, the influence of
osteogenic differentiation
in vitro
on the immunological characteristics of BMSCs and ASCs is the subject
of this article.
Before and after osteogenic induction, the influence of BMSCs and ASCs on the proliferative behavior of
resting and activated allogenic peripheral blood mononuclear cells (PBMCs) was studied as a measure of
the immune response (mixed lymphocyte culture). At the same points, the expression of immunologically
relevant surface markers (e.g., major histocompatibility complex (MHC)-I, MHC-II, CD40, CD40L) was
measured, and correlations between the different sets of results were sought.
The pattern of surface antigen expression of BMSCs is the same as that of ASCs. Analogous to BMSCs,
undifferentiated cells isolated from adipose tissue lack expression ofMHC-II; this is not lost in the course of
the osteogenic differentiation process. In co-culture with allogenic PBMCs, both cell types fail to lead to any
significant stimulation, and they both retain these characteristics during the differentiation process. BMSCs
and ASCs suppress proliferation on activated PBMCs before and after osteogenic differentiation.
Our results confirm that MSCs are immune modulating cells. These properties are retained even after
osteogenic induction
in vitro
and seem to be similar in BMSCs and ASCs. Our results suggest that allogenic
transplantation of BMSCs and ASCs would be possible, for example, in the context of tissue engineering.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>17518585</pmid><doi>10.1089/ten.2006.0114</doi><tpages>11</tpages></addata></record> |
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| ispartof | Tissue engineering, 2007-01, Vol.13 (1), p.111-121 |
| issn | 1076-3279 1557-8690 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Adipose Tissue - cytology Adipose Tissue - immunology Adipose Tissue - metabolism Antigens, CD - biosynthesis Bone Marrow Cells - cytology Bone Marrow Cells - immunology Bone Marrow Cells - metabolism Cell Communication - immunology Cell Differentiation - immunology Cells, Cultured Histocompatibility Antigens Class I - biosynthesis Histocompatibility Antigens Class II - biosynthesis Humans Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - metabolism Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - immunology Mesenchymal Stromal Cells - metabolism Osteogenesis - immunology Stromal Cells - cytology Stromal Cells - immunology Stromal Cells - metabolism T-Lymphocytes, Regulatory - cytology T-Lymphocytes, Regulatory - immunology T-Lymphocytes, Regulatory - metabolism Time Factors |
| title | Comparison of Immunological Properties of Bone Marrow Stromal Cells and Adipose Tissue-Derived Stem Cells Before and After Osteogenic Differentiation In Vitro |
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