Isolation and characterization of proteoglycans synthesized by rat myoblasts L6J1 in culture

Proteoglycans synthesized by rat myoblasts L6J1 in culture were isolated using sorbent Q-Sepharose from culture medium, extracellular matrix (ECM), and cells. Elution of the sorbed material in a NaCl gradient separated proteoglycans from the bulk of proteins eluted at low concentration of the salt....

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Veröffentlicht in:	Biochemistry (Moscow) 2007-04, Vol.72 (4), p.452-458
Hauptverfasser:	Ermakova, I I, Mokrushin, A L, Chertkova, T A, Sakuta, G A, Romaniouk, A V, Morozov, V I
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Carbohydrates - analysis Cell culture Cells, Cultured Chemical properties Electrophoresis, Polyacrylamide Gel Muscle cells Myoblasts - metabolism Physiological aspects Properties Proteins Proteoglycans Proteoglycans - chemistry Proteoglycans - isolation & purification Rats Rattus Sodium chloride
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