Ectopic expression of Dahlia merckii defensin DmAMP1 improves papaya resistance to Phytophthora palmivora by reducing pathogen vigor
Phytophthora spp., some of the more important casual agents of plant diseases, are responsible for heavy economic losses worldwide. Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However,...
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| Veröffentlicht in: | Planta 2007-06, Vol.226 (1), p.87-97 |
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| creator | Zhu, Yun J Agbayani, Ricelle Moore, Paul H |
| description | Phytophthora spp., some of the more important casual agents of plant diseases, are responsible for heavy economic losses worldwide. Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However, the effectiveness and mechanism of interaction of the defensins with Phytophthora spp. have not been clearly characterized in planta. In this study, we expressed the Dahlia merckii defensin, DmAMP1, in papaya (Carica papaya L.), a plant highly susceptible to a root, stem, and fruit rot disease caused by Phytophthora palmivora. Extracts of total leaf proteins from transformed plants inhibited growth of Phytophthora in vitro and discs cut from the leaves of transformed plants inhibited growth of Phytophthora in a bioassay. Results from our greenhouse inoculation experiments demonstrate that expressing the DmAMP1 gene in papaya plants increased resistance against P. palmivora and that this increased resistance was associated with reduced hyphae growth of P. palmivora at the infection sites. The inhibitory effects of DmAMP1 expression in papaya suggest this approach has good potential to impart transgenic resistance against Phytophthora in papaya. |
| doi_str_mv | 10.1007/s00425-006-0471-1 |
| format | Article |
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Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However, the effectiveness and mechanism of interaction of the defensins with Phytophthora spp. have not been clearly characterized in planta. In this study, we expressed the Dahlia merckii defensin, DmAMP1, in papaya (Carica papaya L.), a plant highly susceptible to a root, stem, and fruit rot disease caused by Phytophthora palmivora. Extracts of total leaf proteins from transformed plants inhibited growth of Phytophthora in vitro and discs cut from the leaves of transformed plants inhibited growth of Phytophthora in a bioassay. Results from our greenhouse inoculation experiments demonstrate that expressing the DmAMP1 gene in papaya plants increased resistance against P. palmivora and that this increased resistance was associated with reduced hyphae growth of P. palmivora at the infection sites. The inhibitory effects of DmAMP1 expression in papaya suggest this approach has good potential to impart transgenic resistance against Phytophthora in papaya.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-006-0471-1</identifier><identifier>PMID: 17216480</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Bioassays ; Biological and medical sciences ; Callus ; Carica - genetics ; Carica - microbiology ; Carica papaya ; Crop diseases ; Dahlia - genetics ; Defensins ; Defensins - genetics ; Economic impact ; Ectopic expression ; Fruit rot ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; genetic transformation ; Hyphae ; Immunity, Innate ; Inoculation ; Leaves ; Papayas ; Pathogens ; Pathology, epidemiology, host-fungus relationships. Damages, economic importance ; Phytopathology. Animal pests. Plant and forest protection ; Phytophthora ; Phytophthora - pathogenicity ; Phytophthora palmivora ; Plant diseases ; Plant Diseases - microbiology ; Plant microbe interaction ; Plant roots ; Plants ; Plants, Genetically Modified - microbiology ; root rot ; Transgenes ; Transgenic plants ; Zoospores</subject><ispartof>Planta, 2007-06, Vol.226 (1), p.87-97</ispartof><rights>Springer-Verlag Berlin Heidelberg 2007</rights><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-15fbc92cc67f9a6d8a44e91b6441a716cf8f6c494d98111ab7cc6544b197bf5d3</citedby><cites>FETCH-LOGICAL-c402t-15fbc92cc67f9a6d8a44e91b6441a716cf8f6c494d98111ab7cc6544b197bf5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23389652$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23389652$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18796845$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17216480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Yun J</creatorcontrib><creatorcontrib>Agbayani, Ricelle</creatorcontrib><creatorcontrib>Moore, Paul H</creatorcontrib><title>Ectopic expression of Dahlia merckii defensin DmAMP1 improves papaya resistance to Phytophthora palmivora by reducing pathogen vigor</title><title>Planta</title><addtitle>Planta</addtitle><description>Phytophthora spp., some of the more important casual agents of plant diseases, are responsible for heavy economic losses worldwide. Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However, the effectiveness and mechanism of interaction of the defensins with Phytophthora spp. have not been clearly characterized in planta. In this study, we expressed the Dahlia merckii defensin, DmAMP1, in papaya (Carica papaya L.), a plant highly susceptible to a root, stem, and fruit rot disease caused by Phytophthora palmivora. Extracts of total leaf proteins from transformed plants inhibited growth of Phytophthora in vitro and discs cut from the leaves of transformed plants inhibited growth of Phytophthora in a bioassay. Results from our greenhouse inoculation experiments demonstrate that expressing the DmAMP1 gene in papaya plants increased resistance against P. palmivora and that this increased resistance was associated with reduced hyphae growth of P. palmivora at the infection sites. The inhibitory effects of DmAMP1 expression in papaya suggest this approach has good potential to impart transgenic resistance against Phytophthora in papaya.</description><subject>Bioassays</subject><subject>Biological and medical sciences</subject><subject>Callus</subject><subject>Carica - genetics</subject><subject>Carica - microbiology</subject><subject>Carica papaya</subject><subject>Crop diseases</subject><subject>Dahlia - genetics</subject><subject>Defensins</subject><subject>Defensins - genetics</subject><subject>Economic impact</subject><subject>Ectopic expression</subject><subject>Fruit rot</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>genetic transformation</subject><subject>Hyphae</subject><subject>Immunity, Innate</subject><subject>Inoculation</subject><subject>Leaves</subject><subject>Papayas</subject><subject>Pathogens</subject><subject>Pathology, epidemiology, host-fungus relationships. Damages, economic importance</subject><subject>Phytopathology. Animal pests. 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Plant defensins have been introduced as transgenes into a range of species to increase host resistance to pathogens to which they were originally susceptible. However, the effectiveness and mechanism of interaction of the defensins with Phytophthora spp. have not been clearly characterized in planta. In this study, we expressed the Dahlia merckii defensin, DmAMP1, in papaya (Carica papaya L.), a plant highly susceptible to a root, stem, and fruit rot disease caused by Phytophthora palmivora. Extracts of total leaf proteins from transformed plants inhibited growth of Phytophthora in vitro and discs cut from the leaves of transformed plants inhibited growth of Phytophthora in a bioassay. Results from our greenhouse inoculation experiments demonstrate that expressing the DmAMP1 gene in papaya plants increased resistance against P. palmivora and that this increased resistance was associated with reduced hyphae growth of P. palmivora at the infection sites. The inhibitory effects of DmAMP1 expression in papaya suggest this approach has good potential to impart transgenic resistance against Phytophthora in papaya.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17216480</pmid><doi>10.1007/s00425-006-0471-1</doi><tpages>11</tpages></addata></record> |
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| ispartof | Planta, 2007-06, Vol.226 (1), p.87-97 |
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| subjects | Bioassays Biological and medical sciences Callus Carica - genetics Carica - microbiology Carica papaya Crop diseases Dahlia - genetics Defensins Defensins - genetics Economic impact Ectopic expression Fruit rot Fundamental and applied biological sciences. Psychology Fungal plant pathogens genetic transformation Hyphae Immunity, Innate Inoculation Leaves Papayas Pathogens Pathology, epidemiology, host-fungus relationships. Damages, economic importance Phytopathology. Animal pests. Plant and forest protection Phytophthora Phytophthora - pathogenicity Phytophthora palmivora Plant diseases Plant Diseases - microbiology Plant microbe interaction Plant roots Plants Plants, Genetically Modified - microbiology root rot Transgenes Transgenic plants Zoospores |
| title | Ectopic expression of Dahlia merckii defensin DmAMP1 improves papaya resistance to Phytophthora palmivora by reducing pathogen vigor |
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