Chromate reduction by Burkholderia cepacia MCMB-821,isolated from the pristine habitat of alkaline crater lake
The Cr(VI)-reducing bacterial strain MCMB-821 was isolated from the alkaline crater lake of Lonar and was identified as Burkholderia cepacia. MCMB-821 was resistant to 1,000-ppm Cr(VI) and reduced 98% of the 75 ppm Cr(VI) within 36 h at pH 9.0 in the presence of 2% salt and lactose as the electron d...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2007-06, Vol.75 (3), p.627-632 |
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| creator | Wani, Revati Kodam, K. M Gawai, K. R Dhakephalkar, P. K |
| description | The Cr(VI)-reducing bacterial strain MCMB-821 was isolated from the alkaline crater lake of Lonar and was identified as Burkholderia cepacia. MCMB-821 was resistant to 1,000-ppm Cr(VI) and reduced 98% of the 75 ppm Cr(VI) within 36 h at pH 9.0 in the presence of 2% salt and lactose as the electron donor. The chromate-reducing efficiency of MCMB-821 was comparable under both aerobic as well as anaerobic conditions. Electron paramagnetic resonance spectroscopy data suggested that MCMB-821 reduced Cr(VI) to Cr(III) via the formation of transient Cr(V) intermediate. The chromate-reducing ability of MCMB-821 was suppressed in the presence of membrane inhibitors and enhanced in the presence of 2,4-dinitrophenol, suggesting the involvement of electron transport chain in the Cr(VI) bioreduction. |
| doi_str_mv | 10.1007/s00253-007-0862-7 |
| format | Article |
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The chromate-reducing ability of MCMB-821 was suppressed in the presence of membrane inhibitors and enhanced in the presence of 2,4-dinitrophenol, suggesting the involvement of electron transport chain in the Cr(VI) bioreduction.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-007-0862-7</identifier><identifier>PMID: 17361433</identifier><identifier>CODEN: AMBIDG</identifier><language>eng</language><publisher>Berlin: Berlin/Heidelberg : Springer-Verlag</publisher><subject>2,4-Dinitrophenol - pharmacology ; Anaerobic conditions ; Antimycin A - pharmacology ; Azides - pharmacology ; Bioavailability ; Biological and medical sciences ; Bioremediation ; Biotechnology ; Burkholderia ; Burkholderia cepacia ; Burkholderia cepacia - isolation & purification ; Burkholderia cepacia - metabolism ; Cellular biology ; Chromate reduction ; Chromates - chemistry ; Chromates - metabolism ; Chromium ; Efficiency ; Electron Spin Resonance Spectroscopy ; Electron Transport - drug effects ; Electrons ; Fresh Water - microbiology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Habitats ; Hydrogen-Ion Concentration ; Lactose ; Lakes ; Oxidation-Reduction ; Proteins ; Resistance and optimization of bioreduction ; Statistical analysis ; Studies</subject><ispartof>Applied microbiology and biotechnology, 2007-06, Vol.75 (3), p.627-632</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-ddb659bebc442ac8e131b49b7f2e1ca8a7aeca9dd3638ed09d1fe0991e8540d23</citedby><cites>FETCH-LOGICAL-c411t-ddb659bebc442ac8e131b49b7f2e1ca8a7aeca9dd3638ed09d1fe0991e8540d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18799932$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17361433$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wani, Revati</creatorcontrib><creatorcontrib>Kodam, K. M</creatorcontrib><creatorcontrib>Gawai, K. R</creatorcontrib><creatorcontrib>Dhakephalkar, P. K</creatorcontrib><title>Chromate reduction by Burkholderia cepacia MCMB-821,isolated from the pristine habitat of alkaline crater lake</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><description>The Cr(VI)-reducing bacterial strain MCMB-821 was isolated from the alkaline crater lake of Lonar and was identified as Burkholderia cepacia. MCMB-821 was resistant to 1,000-ppm Cr(VI) and reduced 98% of the 75 ppm Cr(VI) within 36 h at pH 9.0 in the presence of 2% salt and lactose as the electron donor. The chromate-reducing efficiency of MCMB-821 was comparable under both aerobic as well as anaerobic conditions. Electron paramagnetic resonance spectroscopy data suggested that MCMB-821 reduced Cr(VI) to Cr(III) via the formation of transient Cr(V) intermediate. The chromate-reducing ability of MCMB-821 was suppressed in the presence of membrane inhibitors and enhanced in the presence of 2,4-dinitrophenol, suggesting the involvement of electron transport chain in the Cr(VI) bioreduction.</description><subject>2,4-Dinitrophenol - pharmacology</subject><subject>Anaerobic conditions</subject><subject>Antimycin A - pharmacology</subject><subject>Azides - pharmacology</subject><subject>Bioavailability</subject><subject>Biological and medical sciences</subject><subject>Bioremediation</subject><subject>Biotechnology</subject><subject>Burkholderia</subject><subject>Burkholderia cepacia</subject><subject>Burkholderia cepacia - isolation & purification</subject><subject>Burkholderia cepacia - metabolism</subject><subject>Cellular biology</subject><subject>Chromate reduction</subject><subject>Chromates - chemistry</subject><subject>Chromates - metabolism</subject><subject>Chromium</subject><subject>Efficiency</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Electron Transport - drug effects</subject><subject>Electrons</subject><subject>Fresh Water - microbiology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. 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M</au><au>Gawai, K. R</au><au>Dhakephalkar, P. K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chromate reduction by Burkholderia cepacia MCMB-821,isolated from the pristine habitat of alkaline crater lake</atitle><jtitle>Applied microbiology and biotechnology</jtitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>75</volume><issue>3</issue><spage>627</spage><epage>632</epage><pages>627-632</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><coden>AMBIDG</coden><abstract>The Cr(VI)-reducing bacterial strain MCMB-821 was isolated from the alkaline crater lake of Lonar and was identified as Burkholderia cepacia. MCMB-821 was resistant to 1,000-ppm Cr(VI) and reduced 98% of the 75 ppm Cr(VI) within 36 h at pH 9.0 in the presence of 2% salt and lactose as the electron donor. The chromate-reducing efficiency of MCMB-821 was comparable under both aerobic as well as anaerobic conditions. Electron paramagnetic resonance spectroscopy data suggested that MCMB-821 reduced Cr(VI) to Cr(III) via the formation of transient Cr(V) intermediate. The chromate-reducing ability of MCMB-821 was suppressed in the presence of membrane inhibitors and enhanced in the presence of 2,4-dinitrophenol, suggesting the involvement of electron transport chain in the Cr(VI) bioreduction.</abstract><cop>Berlin</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>17361433</pmid><doi>10.1007/s00253-007-0862-7</doi><tpages>6</tpages></addata></record> |
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| subjects | 2,4-Dinitrophenol - pharmacology Anaerobic conditions Antimycin A - pharmacology Azides - pharmacology Bioavailability Biological and medical sciences Bioremediation Biotechnology Burkholderia Burkholderia cepacia Burkholderia cepacia - isolation & purification Burkholderia cepacia - metabolism Cellular biology Chromate reduction Chromates - chemistry Chromates - metabolism Chromium Efficiency Electron Spin Resonance Spectroscopy Electron Transport - drug effects Electrons Fresh Water - microbiology Freshwater Fundamental and applied biological sciences. Psychology Habitats Hydrogen-Ion Concentration Lactose Lakes Oxidation-Reduction Proteins Resistance and optimization of bioreduction Statistical analysis Studies |
| title | Chromate reduction by Burkholderia cepacia MCMB-821,isolated from the pristine habitat of alkaline crater lake |
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