A Mechanism for Antibody-mediated Outside-in Activation of LFA-1

MEM83 is an inserted domain (I-domain)-specific antibody that up-regulates the interaction of LFA-1 with ICAM-1 through an outside-in activation mechanism. We demonstrate here that there is no change in the affinity of the MEM83 antibody for the I-domain in either its low (wild-type) or high affinit...

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Veröffentlicht in:	The Journal of biological chemistry 2008-04, Vol.283 (16), p.10642-10648
Hauptverfasser:	Carreño, Roberto, Li, Dan, Sen, Mehmet, Nira, Iris, Yamakawa, Tatsuo, Ma, Qing, Legge, Glen B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - chemistry Cell Line Humans Immunoglobulin G - chemistry Intercellular Adhesion Molecule-1 - metabolism Kinetics Lymphocyte Function-Associated Antigen-1 - metabolism Models, Biological Models, Molecular Molecular Conformation Protein Binding Protein Conformation Protein Structure, Tertiary Surface Plasmon Resonance Thermodynamics
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