Dysregulated growth factor gene expression is associated with tubulointerstitial apoptosis and renal dysfunction
Chronic renal disease is characterized by declining renal function, loss of intrinsic renal cells, and their replacement with fibrotic tissue. This study investigates apoptosis and its regulation in the context of chronic renal disease. RNA was extracted from renal biopsies from patients with variou...
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| Veröffentlicht in: | Kidney international 2007-05, Vol.71 (10), p.1044-1053 |
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| creator | Teteris, S.A. Menahem, S.A. Perry, G. Maguire, J.A. Dowling, J.P. Langham, R.G. Thomson, N.M. Stein, A.N. |
| description | Chronic renal disease is characterized by declining renal function, loss of intrinsic renal cells, and their replacement with fibrotic tissue. This study investigates apoptosis and its regulation in the context of chronic renal disease. RNA was extracted from renal biopsies from patients with various forms of chronic renal disease. Expression of genes of the Bcl-2 family, death receptor pathway, and growth factors were measured by reverse-transcription real-time polymerase chain reaction. Apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling technique. Tubulointerstitial apoptosis was positively associated with tubulointerstitial injury and renal dysfunction and increased 2.3-fold per unit (U) increase in transforming growth factor β1 (TGFβ1) mRNA (P |
| doi_str_mv | 10.1038/sj.ki.5002176 |
| format | Article |
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This study investigates apoptosis and its regulation in the context of chronic renal disease. RNA was extracted from renal biopsies from patients with various forms of chronic renal disease. Expression of genes of the Bcl-2 family, death receptor pathway, and growth factors were measured by reverse-transcription real-time polymerase chain reaction. Apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling technique. Tubulointerstitial apoptosis was positively associated with tubulointerstitial injury and renal dysfunction and increased 2.3-fold per unit (U) increase in transforming growth factor β1 (TGFβ1) mRNA (P<0.05). Conversely, a 1U increase in epidermal growth factor (EGF) mRNA was associated with a 47% decrease in tubulointerstitial apoptosis (P<0.05). Tubulointerstitial injury was correlated with increased TGFβ1 and tumour necrosis factor α (TNFα) mRNA (P<0.005) and decreased EGF mRNA (P<0.05). Additionally, for a 10U decrease in the glomerular filtration rate there was an estimated increase of 5 and 10% in TGFβ1 and TNFα mRNA, respectively (P<0.05), whereas EGF mRNA decreased by an estimated 15% (P<0.005). Therefore dysregulation of cytokine/growth factor expression plays a central role in the progression of chronic renal disease through contribution to renal cell loss, tubulointerstitial injury, and renal dysfunction.]]></description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1038/sj.ki.5002176</identifier><identifier>PMID: 17361117</identifier><identifier>CODEN: KDYIA5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Adult ; Aged ; Aged, 80 and over ; apoptosis ; Apoptosis - genetics ; Biological and medical sciences ; Biomarkers - blood ; Biopsy ; Chronic Disease ; chronic renal disease ; epidermal growth factor ; Epidermal Growth Factor - genetics ; Female ; Gene Expression ; Gene Expression Regulation ; Glomerular Filtration Rate ; Humans ; Intercellular Signaling Peptides and Proteins - genetics ; Kidney - pathology ; Kidney - physiopathology ; Kidney Diseases - complications ; Kidney Diseases - genetics ; Kidney Diseases - pathology ; Kidney Diseases - physiopathology ; Kidney Tubules - physiopathology ; Kidneys ; Male ; Medical sciences ; Middle Aged ; Nephrology. Urinary tract diseases ; Nephropathies. Renovascular diseases. Renal failure ; Proteinuria - etiology ; Renal failure ; RNA, Messenger - metabolism ; TGF beta ; TNF alpha ; Transforming Growth Factor beta1 - genetics ; Treatment Outcome ; Tumor Necrosis Factor-alpha - genetics ; Urinary system involvement in other diseases. Miscellaneous</subject><ispartof>Kidney international, 2007-05, Vol.71 (10), p.1044-1053</ispartof><rights>2007 International Society of Nephrology</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Nature Publishing Group May 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-b6e9c980a7c07a98c6b01fbe91e19d9f8325cd9fb2fdb3945bbdc3ecaba68bbe3</citedby><cites>FETCH-LOGICAL-c435t-b6e9c980a7c07a98c6b01fbe91e19d9f8325cd9fb2fdb3945bbdc3ecaba68bbe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18999704$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17361117$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teteris, S.A.</creatorcontrib><creatorcontrib>Menahem, S.A.</creatorcontrib><creatorcontrib>Perry, G.</creatorcontrib><creatorcontrib>Maguire, J.A.</creatorcontrib><creatorcontrib>Dowling, J.P.</creatorcontrib><creatorcontrib>Langham, R.G.</creatorcontrib><creatorcontrib>Thomson, N.M.</creatorcontrib><creatorcontrib>Stein, A.N.</creatorcontrib><title>Dysregulated growth factor gene expression is associated with tubulointerstitial apoptosis and renal dysfunction</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description><![CDATA[Chronic renal disease is characterized by declining renal function, loss of intrinsic renal cells, and their replacement with fibrotic tissue. This study investigates apoptosis and its regulation in the context of chronic renal disease. RNA was extracted from renal biopsies from patients with various forms of chronic renal disease. Expression of genes of the Bcl-2 family, death receptor pathway, and growth factors were measured by reverse-transcription real-time polymerase chain reaction. Apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling technique. Tubulointerstitial apoptosis was positively associated with tubulointerstitial injury and renal dysfunction and increased 2.3-fold per unit (U) increase in transforming growth factor β1 (TGFβ1) mRNA (P<0.05). Conversely, a 1U increase in epidermal growth factor (EGF) mRNA was associated with a 47% decrease in tubulointerstitial apoptosis (P<0.05). Tubulointerstitial injury was correlated with increased TGFβ1 and tumour necrosis factor α (TNFα) mRNA (P<0.005) and decreased EGF mRNA (P<0.05). Additionally, for a 10U decrease in the glomerular filtration rate there was an estimated increase of 5 and 10% in TGFβ1 and TNFα mRNA, respectively (P<0.05), whereas EGF mRNA decreased by an estimated 15% (P<0.005). Therefore dysregulation of cytokine/growth factor expression plays a central role in the progression of chronic renal disease through contribution to renal cell loss, tubulointerstitial injury, and renal dysfunction.]]></description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>apoptosis</subject><subject>Apoptosis - genetics</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - blood</subject><subject>Biopsy</subject><subject>Chronic Disease</subject><subject>chronic renal disease</subject><subject>epidermal growth factor</subject><subject>Epidermal Growth Factor - genetics</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gene Expression Regulation</subject><subject>Glomerular Filtration Rate</subject><subject>Humans</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Kidney - pathology</subject><subject>Kidney - physiopathology</subject><subject>Kidney Diseases - complications</subject><subject>Kidney Diseases - genetics</subject><subject>Kidney Diseases - pathology</subject><subject>Kidney Diseases - physiopathology</subject><subject>Kidney Tubules - physiopathology</subject><subject>Kidneys</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Nephropathies. Renovascular diseases. Renal failure</subject><subject>Proteinuria - etiology</subject><subject>Renal failure</subject><subject>RNA, Messenger - metabolism</subject><subject>TGF beta</subject><subject>TNF alpha</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Treatment Outcome</subject><subject>Tumor Necrosis Factor-alpha - genetics</subject><subject>Urinary system involvement in other diseases. 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Urinary tract diseases</topic><topic>Nephropathies. Renovascular diseases. Renal failure</topic><topic>Proteinuria - etiology</topic><topic>Renal failure</topic><topic>RNA, Messenger - metabolism</topic><topic>TGF beta</topic><topic>TNF alpha</topic><topic>Transforming Growth Factor beta1 - genetics</topic><topic>Treatment Outcome</topic><topic>Tumor Necrosis Factor-alpha - genetics</topic><topic>Urinary system involvement in other diseases. Miscellaneous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teteris, S.A.</creatorcontrib><creatorcontrib>Menahem, S.A.</creatorcontrib><creatorcontrib>Perry, G.</creatorcontrib><creatorcontrib>Maguire, J.A.</creatorcontrib><creatorcontrib>Dowling, J.P.</creatorcontrib><creatorcontrib>Langham, R.G.</creatorcontrib><creatorcontrib>Thomson, N.M.</creatorcontrib><creatorcontrib>Stein, A.N.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teteris, S.A.</au><au>Menahem, S.A.</au><au>Perry, G.</au><au>Maguire, J.A.</au><au>Dowling, J.P.</au><au>Langham, R.G.</au><au>Thomson, N.M.</au><au>Stein, A.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dysregulated growth factor gene expression is associated with tubulointerstitial apoptosis and renal dysfunction</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>71</volume><issue>10</issue><spage>1044</spage><epage>1053</epage><pages>1044-1053</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><coden>KDYIA5</coden><abstract><![CDATA[Chronic renal disease is characterized by declining renal function, loss of intrinsic renal cells, and their replacement with fibrotic tissue. This study investigates apoptosis and its regulation in the context of chronic renal disease. RNA was extracted from renal biopsies from patients with various forms of chronic renal disease. Expression of genes of the Bcl-2 family, death receptor pathway, and growth factors were measured by reverse-transcription real-time polymerase chain reaction. Apoptosis was detected by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling technique. Tubulointerstitial apoptosis was positively associated with tubulointerstitial injury and renal dysfunction and increased 2.3-fold per unit (U) increase in transforming growth factor β1 (TGFβ1) mRNA (P<0.05). Conversely, a 1U increase in epidermal growth factor (EGF) mRNA was associated with a 47% decrease in tubulointerstitial apoptosis (P<0.05). Tubulointerstitial injury was correlated with increased TGFβ1 and tumour necrosis factor α (TNFα) mRNA (P<0.005) and decreased EGF mRNA (P<0.05). Additionally, for a 10U decrease in the glomerular filtration rate there was an estimated increase of 5 and 10% in TGFβ1 and TNFα mRNA, respectively (P<0.05), whereas EGF mRNA decreased by an estimated 15% (P<0.005). Therefore dysregulation of cytokine/growth factor expression plays a central role in the progression of chronic renal disease through contribution to renal cell loss, tubulointerstitial injury, and renal dysfunction.]]></abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>17361117</pmid><doi>10.1038/sj.ki.5002176</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Aged Aged, 80 and over apoptosis Apoptosis - genetics Biological and medical sciences Biomarkers - blood Biopsy Chronic Disease chronic renal disease epidermal growth factor Epidermal Growth Factor - genetics Female Gene Expression Gene Expression Regulation Glomerular Filtration Rate Humans Intercellular Signaling Peptides and Proteins - genetics Kidney - pathology Kidney - physiopathology Kidney Diseases - complications Kidney Diseases - genetics Kidney Diseases - pathology Kidney Diseases - physiopathology Kidney Tubules - physiopathology Kidneys Male Medical sciences Middle Aged Nephrology. Urinary tract diseases Nephropathies. Renovascular diseases. Renal failure Proteinuria - etiology Renal failure RNA, Messenger - metabolism TGF beta TNF alpha Transforming Growth Factor beta1 - genetics Treatment Outcome Tumor Necrosis Factor-alpha - genetics Urinary system involvement in other diseases. Miscellaneous |
| title | Dysregulated growth factor gene expression is associated with tubulointerstitial apoptosis and renal dysfunction |
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