Expression of α-synuclein, a presynaptic protein implicated in Parkinson’s disease, in erythropoietic lineage
The present study investigated expression of α-synuclein (α-syn), a presynaptic protein involved in the pathogenesis of Parkinson’s disease, in erythroid cells. Using various immunological procedures, immunoreactivity of α-syn was unambiguously demonstrated in erythroid lineage in murine bone marrow...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-06, Vol.358 (1), p.104-110 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Nakai, Masaaki Fujita, Masayo Waragai, Masaaki Sugama, Shuei Wei, Jianshe Akatsu, Hiroyasu Ohtaka-Maruyama, Chiaki Okado, Haruo Hashimoto, Makoto |
| description | The present study investigated expression of α-synuclein (α-syn), a presynaptic protein involved in the pathogenesis of Parkinson’s disease, in erythroid cells. Using various immunological procedures, immunoreactivity of α-syn was unambiguously demonstrated in erythroid lineage in murine bone marrows and peripheral erythrocytes. Expression of α-syn mRNA was also confirmed by
in situ hybridization. Furthermore, flow cytometry analysis revealed that approximately 80% of erythroid cells in murine bone marrows expressed α-syn, while more than 90% of peripheral erythrocytes expressed α-syn. Nonetheless, α-syn null mice exhibited apparently no phenotypic changes in erythroid cells as was the case in their brains, suggesting that there might be underlying some redundant mechanisms. Together with previous reports showing the expression of α-syn in lymphocytes and platelets, the present finding supports a contention that α-syn might play some important functions in hematopoietic system. |
| doi_str_mv | 10.1016/j.bbrc.2007.04.108 |
| format | Article |
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in situ hybridization. Furthermore, flow cytometry analysis revealed that approximately 80% of erythroid cells in murine bone marrows expressed α-syn, while more than 90% of peripheral erythrocytes expressed α-syn. Nonetheless, α-syn null mice exhibited apparently no phenotypic changes in erythroid cells as was the case in their brains, suggesting that there might be underlying some redundant mechanisms. Together with previous reports showing the expression of α-syn in lymphocytes and platelets, the present finding supports a contention that α-syn might play some important functions in hematopoietic system.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2007.04.108</identifier><identifier>PMID: 17475220</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>alpha-Synuclein - biosynthesis ; alpha-Synuclein - genetics ; Animals ; Bone Marrow - metabolism ; Bone marrows ; Brain - metabolism ; Cell Lineage - physiology ; DJ-1 ; Erythroblasts ; Erythrocytes ; Erythrocytes - metabolism ; Erythroid Cells - metabolism ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Parkinson Disease - metabolism ; Parkinson’s disease ; α-Synuclein</subject><ispartof>Biochemical and biophysical research communications, 2007-06, Vol.358 (1), p.104-110</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-fdaa89a1b5873f7d93b4b007c4598816cb9b86fd55e6406f707061aab8a9181d3</citedby><cites>FETCH-LOGICAL-c385t-fdaa89a1b5873f7d93b4b007c4598816cb9b86fd55e6406f707061aab8a9181d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2007.04.108$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17475220$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakai, Masaaki</creatorcontrib><creatorcontrib>Fujita, Masayo</creatorcontrib><creatorcontrib>Waragai, Masaaki</creatorcontrib><creatorcontrib>Sugama, Shuei</creatorcontrib><creatorcontrib>Wei, Jianshe</creatorcontrib><creatorcontrib>Akatsu, Hiroyasu</creatorcontrib><creatorcontrib>Ohtaka-Maruyama, Chiaki</creatorcontrib><creatorcontrib>Okado, Haruo</creatorcontrib><creatorcontrib>Hashimoto, Makoto</creatorcontrib><title>Expression of α-synuclein, a presynaptic protein implicated in Parkinson’s disease, in erythropoietic lineage</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The present study investigated expression of α-synuclein (α-syn), a presynaptic protein involved in the pathogenesis of Parkinson’s disease, in erythroid cells. Using various immunological procedures, immunoreactivity of α-syn was unambiguously demonstrated in erythroid lineage in murine bone marrows and peripheral erythrocytes. Expression of α-syn mRNA was also confirmed by
in situ hybridization. Furthermore, flow cytometry analysis revealed that approximately 80% of erythroid cells in murine bone marrows expressed α-syn, while more than 90% of peripheral erythrocytes expressed α-syn. Nonetheless, α-syn null mice exhibited apparently no phenotypic changes in erythroid cells as was the case in their brains, suggesting that there might be underlying some redundant mechanisms. Together with previous reports showing the expression of α-syn in lymphocytes and platelets, the present finding supports a contention that α-syn might play some important functions in hematopoietic system.</description><subject>alpha-Synuclein - biosynthesis</subject><subject>alpha-Synuclein - genetics</subject><subject>Animals</subject><subject>Bone Marrow - metabolism</subject><subject>Bone marrows</subject><subject>Brain - metabolism</subject><subject>Cell Lineage - physiology</subject><subject>DJ-1</subject><subject>Erythroblasts</subject><subject>Erythrocytes</subject><subject>Erythrocytes - metabolism</subject><subject>Erythroid Cells - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Parkinson Disease - metabolism</subject><subject>Parkinson’s disease</subject><subject>α-Synuclein</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUuO1DAQtRCI6Rm4AAuUFatJU047_khs0GhgkEaCBUjsLH8q4CZtBzs9ondcg2NwEQ7BSXDULbEDb8r16r0nux4hTyisKVD-fLu2Nrt1ByDWwCom75EVBQVtR4HdJysA4G2n6Mczcl7KFoBSxtVDckYFE33XwYpM19-mjKWEFJs0NL9-tuUQ927EEC8b0yyzQzTTHFy9p7nCTdhNY3BmRt_U7p3JX0IsKf7-_qM0PhQ0BS-XCebD_DmnKQVc5GOIaD7hI_JgMGPBx6d6QT68un5_ddPevn395urlbes2sp_bwRsjlaG2l2IzCK82ltn6Ucd6JSXlzior-eD7HjkDPggQwKkxVhpFJfWbC_Ls6Fuf_XWPZda7UByOo4mY9kUL6EFxJf5LpIoxUU8ldkeiy6mUjIOectiZfNAU9BKI3uolEL0EooFVTFbR05P73u7Q_5WcEqiEF0cC1mXcBcy6uIDRoQ8Z3ax9Cv_y_wOXzJ-d</recordid><startdate>20070622</startdate><enddate>20070622</enddate><creator>Nakai, Masaaki</creator><creator>Fujita, Masayo</creator><creator>Waragai, Masaaki</creator><creator>Sugama, Shuei</creator><creator>Wei, Jianshe</creator><creator>Akatsu, Hiroyasu</creator><creator>Ohtaka-Maruyama, Chiaki</creator><creator>Okado, Haruo</creator><creator>Hashimoto, Makoto</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20070622</creationdate><title>Expression of α-synuclein, a presynaptic protein implicated in Parkinson’s disease, in erythropoietic lineage</title><author>Nakai, Masaaki ; 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in situ hybridization. Furthermore, flow cytometry analysis revealed that approximately 80% of erythroid cells in murine bone marrows expressed α-syn, while more than 90% of peripheral erythrocytes expressed α-syn. Nonetheless, α-syn null mice exhibited apparently no phenotypic changes in erythroid cells as was the case in their brains, suggesting that there might be underlying some redundant mechanisms. Together with previous reports showing the expression of α-syn in lymphocytes and platelets, the present finding supports a contention that α-syn might play some important functions in hematopoietic system.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17475220</pmid><doi>10.1016/j.bbrc.2007.04.108</doi><tpages>7</tpages></addata></record> |
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| subjects | alpha-Synuclein - biosynthesis alpha-Synuclein - genetics Animals Bone Marrow - metabolism Bone marrows Brain - metabolism Cell Lineage - physiology DJ-1 Erythroblasts Erythrocytes Erythrocytes - metabolism Erythroid Cells - metabolism Humans Male Mice Mice, Inbred C57BL Mice, Knockout Parkinson Disease - metabolism Parkinson’s disease α-Synuclein |
| title | Expression of α-synuclein, a presynaptic protein implicated in Parkinson’s disease, in erythropoietic lineage |
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