Isolation and characterization of a haemolysin from Trichophyton mentagrophytes
Haemolytic activities of Trichophyton ( T.) mentagrophytes were detected and characterized by qualitative and quantitative assays. On Columbia agar supplemented with blood from horses, cattle or sheep, T. mentagrophytes expressed a strong zone of complete haemolysis. No haemolytic activities could b...
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| Veröffentlicht in: | Veterinary microbiology 2007-06, Vol.122 (3), p.342-349 |
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| creator | Schaufuss, P. Müller, F. Valentin-Weigand, P. |
| description | Haemolytic activities of
Trichophyton (
T.)
mentagrophytes were detected and characterized by qualitative and quantitative assays. On Columbia agar supplemented with blood from horses, cattle or sheep,
T. mentagrophytes expressed a strong zone of complete haemolysis. No haemolytic activities could be detected in the closely related
T. verrucosum var.
ochraceum. The same results were obtained after cultivation of the fungi on sterile cellulose acetate filters placed on the surface on Columbia blood agar. After removal of the filter, complete haemolysis was detected below the colony of
T. mentagrophytes. A soluble haemolysin from culture supernatant of this strain was isolated and partially purified. Specific haemolytic activity per mg protein was enriched 2.6-fold in filtrate F
1, a fraction obtained as filtrate after filtration through 3
kDa cut-off membranes. The partially purified haemolysin was neither affected by proteinase K treatment, nor by high and low temperatures, suggesting that it represents a small peptide haemolysin. Accordingly, in a commercial enzymatic activity test only the crude culture filtrate, but none of the subsequent purification fractions showed reactivity. Evaluation of the specificity of the haemolysin using erythrocytes from different mammalian species revealed that sensitivity was highest to those of equines, followed by erythrocytes from sheep, cattle, swine, dogs and humans. None of the erythrocytes was lysed by filtrate F
1 from
T. verrucosum var.
ochraceum. Furthermore, different eukaryotic cell lines from different species were tested in their sensitivity to cytolytic activities of the haemolysin, but no membrane damage could be detected. |
| doi_str_mv | 10.1016/j.vetmic.2007.01.022 |
| format | Article |
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Trichophyton (
T.)
mentagrophytes were detected and characterized by qualitative and quantitative assays. On Columbia agar supplemented with blood from horses, cattle or sheep,
T. mentagrophytes expressed a strong zone of complete haemolysis. No haemolytic activities could be detected in the closely related
T. verrucosum var.
ochraceum. The same results were obtained after cultivation of the fungi on sterile cellulose acetate filters placed on the surface on Columbia blood agar. After removal of the filter, complete haemolysis was detected below the colony of
T. mentagrophytes. A soluble haemolysin from culture supernatant of this strain was isolated and partially purified. Specific haemolytic activity per mg protein was enriched 2.6-fold in filtrate F
1, a fraction obtained as filtrate after filtration through 3
kDa cut-off membranes. The partially purified haemolysin was neither affected by proteinase K treatment, nor by high and low temperatures, suggesting that it represents a small peptide haemolysin. Accordingly, in a commercial enzymatic activity test only the crude culture filtrate, but none of the subsequent purification fractions showed reactivity. Evaluation of the specificity of the haemolysin using erythrocytes from different mammalian species revealed that sensitivity was highest to those of equines, followed by erythrocytes from sheep, cattle, swine, dogs and humans. None of the erythrocytes was lysed by filtrate F
1 from
T. verrucosum var.
ochraceum. Furthermore, different eukaryotic cell lines from different species were tested in their sensitivity to cytolytic activities of the haemolysin, but no membrane damage could be detected.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2007.01.022</identifier><identifier>PMID: 17336469</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>animal diseases ; Animals ; assays ; Biological and medical sciences ; Cattle ; Culture Media ; cytotoxicity ; cytotoxins ; dermatomycoses ; Dermatophytes ; detection ; Dogs ; Electrophoresis, Polyacrylamide Gel ; epidemiology ; Erythrocytes - microbiology ; Erythrocytes - physiology ; Fundamental and applied biological sciences. Psychology ; Haemolysis ; Hemolysin Proteins - isolation & purification ; Hemolysin Proteins - pharmacology ; hemolysins ; hemolysis ; Hemolysis - physiology ; Horses ; Human mycoses ; Humans ; hypersensitivity ; in vitro culture ; Infectious diseases ; inflammation ; keratinophilic fungi ; Medical sciences ; Microbiology ; Miscellaneous ; Mycology ; Mycoses ; Mycoses of the skin ; mycotoxins ; pathogenesis ; pathogenicity ; Sensitivity and Specificity ; Sheep ; Species Specificity ; Swine ; Trichophyton ; Trichophyton - metabolism ; Trichophyton - pathogenicity ; Trichophyton mentagrophytes</subject><ispartof>Veterinary microbiology, 2007-06, Vol.122 (3), p.342-349</ispartof><rights>2007 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-5e7954a4b992a5bce9e667a92d09c78d38d615d4cf850cda43c5c309edb88d883</citedby><cites>FETCH-LOGICAL-c491t-5e7954a4b992a5bce9e667a92d09c78d38d615d4cf850cda43c5c309edb88d883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2007.01.022$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18790781$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17336469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schaufuss, P.</creatorcontrib><creatorcontrib>Müller, F.</creatorcontrib><creatorcontrib>Valentin-Weigand, P.</creatorcontrib><title>Isolation and characterization of a haemolysin from Trichophyton mentagrophytes</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Haemolytic activities of
Trichophyton (
T.)
mentagrophytes were detected and characterized by qualitative and quantitative assays. On Columbia agar supplemented with blood from horses, cattle or sheep,
T. mentagrophytes expressed a strong zone of complete haemolysis. No haemolytic activities could be detected in the closely related
T. verrucosum var.
ochraceum. The same results were obtained after cultivation of the fungi on sterile cellulose acetate filters placed on the surface on Columbia blood agar. After removal of the filter, complete haemolysis was detected below the colony of
T. mentagrophytes. A soluble haemolysin from culture supernatant of this strain was isolated and partially purified. Specific haemolytic activity per mg protein was enriched 2.6-fold in filtrate F
1, a fraction obtained as filtrate after filtration through 3
kDa cut-off membranes. The partially purified haemolysin was neither affected by proteinase K treatment, nor by high and low temperatures, suggesting that it represents a small peptide haemolysin. Accordingly, in a commercial enzymatic activity test only the crude culture filtrate, but none of the subsequent purification fractions showed reactivity. Evaluation of the specificity of the haemolysin using erythrocytes from different mammalian species revealed that sensitivity was highest to those of equines, followed by erythrocytes from sheep, cattle, swine, dogs and humans. None of the erythrocytes was lysed by filtrate F
1 from
T. verrucosum var.
ochraceum. Furthermore, different eukaryotic cell lines from different species were tested in their sensitivity to cytolytic activities of the haemolysin, but no membrane damage could be detected.</description><subject>animal diseases</subject><subject>Animals</subject><subject>assays</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Culture Media</subject><subject>cytotoxicity</subject><subject>cytotoxins</subject><subject>dermatomycoses</subject><subject>Dermatophytes</subject><subject>detection</subject><subject>Dogs</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>epidemiology</subject><subject>Erythrocytes - microbiology</subject><subject>Erythrocytes - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Haemolysis</subject><subject>Hemolysin Proteins - isolation & purification</subject><subject>Hemolysin Proteins - pharmacology</subject><subject>hemolysins</subject><subject>hemolysis</subject><subject>Hemolysis - physiology</subject><subject>Horses</subject><subject>Human mycoses</subject><subject>Humans</subject><subject>hypersensitivity</subject><subject>in vitro culture</subject><subject>Infectious diseases</subject><subject>inflammation</subject><subject>keratinophilic fungi</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Mycology</subject><subject>Mycoses</subject><subject>Mycoses of the skin</subject><subject>mycotoxins</subject><subject>pathogenesis</subject><subject>pathogenicity</subject><subject>Sensitivity and Specificity</subject><subject>Sheep</subject><subject>Species Specificity</subject><subject>Swine</subject><subject>Trichophyton</subject><subject>Trichophyton - metabolism</subject><subject>Trichophyton - pathogenicity</subject><subject>Trichophyton mentagrophytes</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E2P1CAYwHFiNO64-g2M9qK31gcoBS4mm40vm2yyB3fPhIGnDpO2jNDZZPz0UjvJ3vREID_gyZ-QtxQaCrT7tG8ecR6DaxiAbIA2wNgzsqFK8pqJlj0nG-BS1ZRycUFe5bwHgFZ38JJcUMl513Z6Q-5uchzsHOJU2clXbmeTdTOm8Hs9jH1lq53FMQ6nHKaqT3Gs7lNwu3jYneYiRpxm-zP93WJ-TV70dsj45rxekoevX-6vv9e3d99urq9ua9dqOtcCpRatbbdaMyu2DjV2nbSaedBOKs-V76jwreuVAOdty51wHDT6rVJeKX5JPq7vHlL8dcQ8mzFkh8NgJ4zHbCQIUFLo_0KqOUhFWYHtCl2KOSfszSGF0aaToWCW4mZv1uJmKW6AmlK8XHt3fv-4HdE_XTonLuDDGdjs7NAnO7mQn5ySehmguPer6200JWgxDz8YUF4-W2It4vMqsIR9DJhMdgEnhz4kdLPxMfx71j9o_KsS</recordid><startdate>20070621</startdate><enddate>20070621</enddate><creator>Schaufuss, P.</creator><creator>Müller, F.</creator><creator>Valentin-Weigand, P.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20070621</creationdate><title>Isolation and characterization of a haemolysin from Trichophyton mentagrophytes</title><author>Schaufuss, P. ; Müller, F. ; Valentin-Weigand, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-5e7954a4b992a5bce9e667a92d09c78d38d615d4cf850cda43c5c309edb88d883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>animal diseases</topic><topic>Animals</topic><topic>assays</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Culture Media</topic><topic>cytotoxicity</topic><topic>cytotoxins</topic><topic>dermatomycoses</topic><topic>Dermatophytes</topic><topic>detection</topic><topic>Dogs</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>epidemiology</topic><topic>Erythrocytes - microbiology</topic><topic>Erythrocytes - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Haemolysis</topic><topic>Hemolysin Proteins - isolation & purification</topic><topic>Hemolysin Proteins - pharmacology</topic><topic>hemolysins</topic><topic>hemolysis</topic><topic>Hemolysis - physiology</topic><topic>Horses</topic><topic>Human mycoses</topic><topic>Humans</topic><topic>hypersensitivity</topic><topic>in vitro culture</topic><topic>Infectious diseases</topic><topic>inflammation</topic><topic>keratinophilic fungi</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Mycology</topic><topic>Mycoses</topic><topic>Mycoses of the skin</topic><topic>mycotoxins</topic><topic>pathogenesis</topic><topic>pathogenicity</topic><topic>Sensitivity and Specificity</topic><topic>Sheep</topic><topic>Species Specificity</topic><topic>Swine</topic><topic>Trichophyton</topic><topic>Trichophyton - metabolism</topic><topic>Trichophyton - pathogenicity</topic><topic>Trichophyton mentagrophytes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schaufuss, P.</creatorcontrib><creatorcontrib>Müller, F.</creatorcontrib><creatorcontrib>Valentin-Weigand, P.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schaufuss, P.</au><au>Müller, F.</au><au>Valentin-Weigand, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of a haemolysin from Trichophyton mentagrophytes</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2007-06-21</date><risdate>2007</risdate><volume>122</volume><issue>3</issue><spage>342</spage><epage>349</epage><pages>342-349</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Haemolytic activities of
Trichophyton (
T.)
mentagrophytes were detected and characterized by qualitative and quantitative assays. On Columbia agar supplemented with blood from horses, cattle or sheep,
T. mentagrophytes expressed a strong zone of complete haemolysis. No haemolytic activities could be detected in the closely related
T. verrucosum var.
ochraceum. The same results were obtained after cultivation of the fungi on sterile cellulose acetate filters placed on the surface on Columbia blood agar. After removal of the filter, complete haemolysis was detected below the colony of
T. mentagrophytes. A soluble haemolysin from culture supernatant of this strain was isolated and partially purified. Specific haemolytic activity per mg protein was enriched 2.6-fold in filtrate F
1, a fraction obtained as filtrate after filtration through 3
kDa cut-off membranes. The partially purified haemolysin was neither affected by proteinase K treatment, nor by high and low temperatures, suggesting that it represents a small peptide haemolysin. Accordingly, in a commercial enzymatic activity test only the crude culture filtrate, but none of the subsequent purification fractions showed reactivity. Evaluation of the specificity of the haemolysin using erythrocytes from different mammalian species revealed that sensitivity was highest to those of equines, followed by erythrocytes from sheep, cattle, swine, dogs and humans. None of the erythrocytes was lysed by filtrate F
1 from
T. verrucosum var.
ochraceum. Furthermore, different eukaryotic cell lines from different species were tested in their sensitivity to cytolytic activities of the haemolysin, but no membrane damage could be detected.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>17336469</pmid><doi>10.1016/j.vetmic.2007.01.022</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Veterinary microbiology, 2007-06, Vol.122 (3), p.342-349 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70508759 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | animal diseases Animals assays Biological and medical sciences Cattle Culture Media cytotoxicity cytotoxins dermatomycoses Dermatophytes detection Dogs Electrophoresis, Polyacrylamide Gel epidemiology Erythrocytes - microbiology Erythrocytes - physiology Fundamental and applied biological sciences. Psychology Haemolysis Hemolysin Proteins - isolation & purification Hemolysin Proteins - pharmacology hemolysins hemolysis Hemolysis - physiology Horses Human mycoses Humans hypersensitivity in vitro culture Infectious diseases inflammation keratinophilic fungi Medical sciences Microbiology Miscellaneous Mycology Mycoses Mycoses of the skin mycotoxins pathogenesis pathogenicity Sensitivity and Specificity Sheep Species Specificity Swine Trichophyton Trichophyton - metabolism Trichophyton - pathogenicity Trichophyton mentagrophytes |
| title | Isolation and characterization of a haemolysin from Trichophyton mentagrophytes |
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