Yeast mutant with efficient secretion identified by a novel secretory reporter, Cluc

Yeast is an important host for the production of pharmaceutical or industrial proteins by virtue of its genetic information and easy handling. A number of heterologous proteins have been produced and purified from yeast cell cultures as secreted forms. Here, we describe a novel screening system of S...

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Veröffentlicht in:	Biochemical and biophysical research communications 2007-06, Vol.358 (2), p.429-434
Hauptverfasser:	Kanjou, Naoko, Nagao, Aki, Ohmiya, Yoshihiro, Ohgiya, Satoru
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals C. noctiluca secretory luciferase (Cluc) Cypridina Cyprinidae - metabolism Genes, Reporter - genetics Genetic Enhancement - methods Luciferases - biosynthesis Luciferases - genetics Mutation - genetics Noctiluca Protein Engineering - methods Saccharomyces cerevisiae Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins - biosynthesis Saccharomyces cerevisiae Proteins - genetics Screening of yeast secretory strains Secretory bioluminescence reporter enzyme
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