Separation and characterization of the colored material from sugarcane molasses
The colored material (X) was effectively separated from sugarcane molasses using reversed-phase chromatography. Characterization of the molecular structure of sample X was performed using infrared absorption (IR) spectrometry, mass spectrometry (MS), and dynamic light scattering (DLS). The IR spectr...
Gespeichert in:
| Veröffentlicht in: | Chemosphere (Oxford) 2008-04, Vol.71 (9), p.1730-1737 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1737 |
|---|---|
| container_issue | 9 |
| container_start_page | 1730 |
| container_title | Chemosphere (Oxford) |
| container_volume | 71 |
| creator | Hatano, Ken-ichi Kikuchi, Satoshi Miyakawa, Takuya Tanokura, Masaru Kubota, Kenji |
| description | The colored material (X) was effectively separated from sugarcane molasses using reversed-phase chromatography. Characterization of the molecular structure of sample X was performed using infrared absorption (IR) spectrometry, mass spectrometry (MS), and dynamic light scattering (DLS). The IR spectrum was similar to that of commercial humic acid, and the MS analysis showed that the sample possessed relatively small heterogeneous molecules with molecular masses around 234, 446, 657, 868, and 1079
Da. On the other hand, X sample showed an inhibitory activity toward the cysteine proteinase papain. Furthermore, the inhibitory (G-1) and weak inhibitory (G-2) fractions were separated from sample X using gel permeation chromatography. Samples G-1 and G-2 inhibited papain partial-noncompetitively and had the inhibition constants of 5.01
×
10
−5 and 1.08
×
10
−3
M, respectively. Interestingly, in the DLS experiment, the Stokes radius of sample G-1 was ∼2
nm, about twice one of sample G-2. |
| doi_str_mv | 10.1016/j.chemosphere.2007.12.019 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70502556</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0045653507015445</els_id><sourcerecordid>70502556</sourcerecordid><originalsourceid>FETCH-LOGICAL-c471t-849f6c0c1bb0c9d3366c63579501196f7b8967a644d2ea5a6b585416434e3e723</originalsourceid><addsrcrecordid>eNqNkM1O3DAURi0Egum0r4DSBewSbMc_8bIaQVsJaRaFteXc3DAeJfHUziCVp8ejGbUsu7ry9fnsT4eQr4xWjDJ1t61gg2NIuw1GrDilumK8osyckQVrtCkZN805WVAqZKlkLa_Ip5S2lOawNJfkijVc6ZrLBVn_wp2LbvZhKtzUFbDJJ5gx-rfjMvTFvMECwhAidsXoDnduKPoYxiLtX1wEN2ExhsGlhOkzuejdkPDLaS7J88P90-pH-bj-_nP17bEEodlcNsL0CiiwtqVgurpWClQttZGUMaN63TZGaaeE6Dg66VQrGymYErXAGjWvl-T2-O4uht97TLMdfQIchlwm7JPVVFIupcqgOYIQQ0oRe7uLfnTxj2XUHmzarf1g0x5sWsZttpmz16dP9u2I3b_kSV8Gbk6AS-CGProJfPrLcco1a4zI3OrIYVby6jHaBB4nwM5HhNl2wf9HnXfZWZkI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70502556</pqid></control><display><type>article</type><title>Separation and characterization of the colored material from sugarcane molasses</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Hatano, Ken-ichi ; Kikuchi, Satoshi ; Miyakawa, Takuya ; Tanokura, Masaru ; Kubota, Kenji</creator><creatorcontrib>Hatano, Ken-ichi ; Kikuchi, Satoshi ; Miyakawa, Takuya ; Tanokura, Masaru ; Kubota, Kenji</creatorcontrib><description>The colored material (X) was effectively separated from sugarcane molasses using reversed-phase chromatography. Characterization of the molecular structure of sample X was performed using infrared absorption (IR) spectrometry, mass spectrometry (MS), and dynamic light scattering (DLS). The IR spectrum was similar to that of commercial humic acid, and the MS analysis showed that the sample possessed relatively small heterogeneous molecules with molecular masses around 234, 446, 657, 868, and 1079
Da. On the other hand, X sample showed an inhibitory activity toward the cysteine proteinase papain. Furthermore, the inhibitory (G-1) and weak inhibitory (G-2) fractions were separated from sample X using gel permeation chromatography. Samples G-1 and G-2 inhibited papain partial-noncompetitively and had the inhibition constants of 5.01
×
10
−5 and 1.08
×
10
−3
M, respectively. Interestingly, in the DLS experiment, the Stokes radius of sample G-1 was ∼2
nm, about twice one of sample G-2.</description><identifier>ISSN: 0045-6535</identifier><identifier>EISSN: 1879-1298</identifier><identifier>DOI: 10.1016/j.chemosphere.2007.12.019</identifier><identifier>PMID: 18267325</identifier><identifier>CODEN: CMSHAF</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Applied sciences ; Biological and medical sciences ; Biological treatment of waters ; Biotechnology ; Chromatography, Liquid ; Electrophoresis, Polyacrylamide Gel ; Environment and pollution ; Exact sciences and technology ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; Hexose alkaline degradation products ; Humic substance ; Industrial applications and implications. Economical aspects ; Industrial wastewaters ; Kinetics ; Light ; Molasses - analysis ; Papain ; Papain - antagonists & inhibitors ; Pollution ; Pollution and sludges ; Proteinase inhibitor ; Reverse-phase chromatography ; Saccharum - chemistry ; Scattering, Radiation ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Spectrophotometry, Infrared ; Spectrophotometry, Ultraviolet ; Stokes radius ; Wastewaters ; Water treatment and pollution</subject><ispartof>Chemosphere (Oxford), 2008-04, Vol.71 (9), p.1730-1737</ispartof><rights>2008 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-849f6c0c1bb0c9d3366c63579501196f7b8967a644d2ea5a6b585416434e3e723</citedby><cites>FETCH-LOGICAL-c471t-849f6c0c1bb0c9d3366c63579501196f7b8967a644d2ea5a6b585416434e3e723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0045653507015445$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20271894$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18267325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hatano, Ken-ichi</creatorcontrib><creatorcontrib>Kikuchi, Satoshi</creatorcontrib><creatorcontrib>Miyakawa, Takuya</creatorcontrib><creatorcontrib>Tanokura, Masaru</creatorcontrib><creatorcontrib>Kubota, Kenji</creatorcontrib><title>Separation and characterization of the colored material from sugarcane molasses</title><title>Chemosphere (Oxford)</title><addtitle>Chemosphere</addtitle><description>The colored material (X) was effectively separated from sugarcane molasses using reversed-phase chromatography. Characterization of the molecular structure of sample X was performed using infrared absorption (IR) spectrometry, mass spectrometry (MS), and dynamic light scattering (DLS). The IR spectrum was similar to that of commercial humic acid, and the MS analysis showed that the sample possessed relatively small heterogeneous molecules with molecular masses around 234, 446, 657, 868, and 1079
Da. On the other hand, X sample showed an inhibitory activity toward the cysteine proteinase papain. Furthermore, the inhibitory (G-1) and weak inhibitory (G-2) fractions were separated from sample X using gel permeation chromatography. Samples G-1 and G-2 inhibited papain partial-noncompetitively and had the inhibition constants of 5.01
×
10
−5 and 1.08
×
10
−3
M, respectively. Interestingly, in the DLS experiment, the Stokes radius of sample G-1 was ∼2
nm, about twice one of sample G-2.</description><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of waters</subject><subject>Biotechnology</subject><subject>Chromatography, Liquid</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Environment and pollution</subject><subject>Exact sciences and technology</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Hexose alkaline degradation products</subject><subject>Humic substance</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Industrial wastewaters</subject><subject>Kinetics</subject><subject>Light</subject><subject>Molasses - analysis</subject><subject>Papain</subject><subject>Papain - antagonists & inhibitors</subject><subject>Pollution</subject><subject>Pollution and sludges</subject><subject>Proteinase inhibitor</subject><subject>Reverse-phase chromatography</subject><subject>Saccharum - chemistry</subject><subject>Scattering, Radiation</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Spectrophotometry, Infrared</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Stokes radius</subject><subject>Wastewaters</subject><subject>Water treatment and pollution</subject><issn>0045-6535</issn><issn>1879-1298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1O3DAURi0Egum0r4DSBewSbMc_8bIaQVsJaRaFteXc3DAeJfHUziCVp8ejGbUsu7ry9fnsT4eQr4xWjDJ1t61gg2NIuw1GrDilumK8osyckQVrtCkZN805WVAqZKlkLa_Ip5S2lOawNJfkijVc6ZrLBVn_wp2LbvZhKtzUFbDJJ5gx-rfjMvTFvMECwhAidsXoDnduKPoYxiLtX1wEN2ExhsGlhOkzuejdkPDLaS7J88P90-pH-bj-_nP17bEEodlcNsL0CiiwtqVgurpWClQttZGUMaN63TZGaaeE6Dg66VQrGymYErXAGjWvl-T2-O4uht97TLMdfQIchlwm7JPVVFIupcqgOYIQQ0oRe7uLfnTxj2XUHmzarf1g0x5sWsZttpmz16dP9u2I3b_kSV8Gbk6AS-CGProJfPrLcco1a4zI3OrIYVby6jHaBB4nwM5HhNl2wf9HnXfZWZkI</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Hatano, Ken-ichi</creator><creator>Kikuchi, Satoshi</creator><creator>Miyakawa, Takuya</creator><creator>Tanokura, Masaru</creator><creator>Kubota, Kenji</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080401</creationdate><title>Separation and characterization of the colored material from sugarcane molasses</title><author>Hatano, Ken-ichi ; Kikuchi, Satoshi ; Miyakawa, Takuya ; Tanokura, Masaru ; Kubota, Kenji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-849f6c0c1bb0c9d3366c63579501196f7b8967a644d2ea5a6b585416434e3e723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biological treatment of waters</topic><topic>Biotechnology</topic><topic>Chromatography, Liquid</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Environment and pollution</topic><topic>Exact sciences and technology</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Hexose alkaline degradation products</topic><topic>Humic substance</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Industrial wastewaters</topic><topic>Kinetics</topic><topic>Light</topic><topic>Molasses - analysis</topic><topic>Papain</topic><topic>Papain - antagonists & inhibitors</topic><topic>Pollution</topic><topic>Pollution and sludges</topic><topic>Proteinase inhibitor</topic><topic>Reverse-phase chromatography</topic><topic>Saccharum - chemistry</topic><topic>Scattering, Radiation</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</topic><topic>Spectrophotometry, Infrared</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Stokes radius</topic><topic>Wastewaters</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hatano, Ken-ichi</creatorcontrib><creatorcontrib>Kikuchi, Satoshi</creatorcontrib><creatorcontrib>Miyakawa, Takuya</creatorcontrib><creatorcontrib>Tanokura, Masaru</creatorcontrib><creatorcontrib>Kubota, Kenji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chemosphere (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hatano, Ken-ichi</au><au>Kikuchi, Satoshi</au><au>Miyakawa, Takuya</au><au>Tanokura, Masaru</au><au>Kubota, Kenji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and characterization of the colored material from sugarcane molasses</atitle><jtitle>Chemosphere (Oxford)</jtitle><addtitle>Chemosphere</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>71</volume><issue>9</issue><spage>1730</spage><epage>1737</epage><pages>1730-1737</pages><issn>0045-6535</issn><eissn>1879-1298</eissn><coden>CMSHAF</coden><abstract>The colored material (X) was effectively separated from sugarcane molasses using reversed-phase chromatography. Characterization of the molecular structure of sample X was performed using infrared absorption (IR) spectrometry, mass spectrometry (MS), and dynamic light scattering (DLS). The IR spectrum was similar to that of commercial humic acid, and the MS analysis showed that the sample possessed relatively small heterogeneous molecules with molecular masses around 234, 446, 657, 868, and 1079
Da. On the other hand, X sample showed an inhibitory activity toward the cysteine proteinase papain. Furthermore, the inhibitory (G-1) and weak inhibitory (G-2) fractions were separated from sample X using gel permeation chromatography. Samples G-1 and G-2 inhibited papain partial-noncompetitively and had the inhibition constants of 5.01
×
10
−5 and 1.08
×
10
−3
M, respectively. Interestingly, in the DLS experiment, the Stokes radius of sample G-1 was ∼2
nm, about twice one of sample G-2.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18267325</pmid><doi>10.1016/j.chemosphere.2007.12.019</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0045-6535 |
| ispartof | Chemosphere (Oxford), 2008-04, Vol.71 (9), p.1730-1737 |
| issn | 0045-6535 1879-1298 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70502556 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Applied sciences Biological and medical sciences Biological treatment of waters Biotechnology Chromatography, Liquid Electrophoresis, Polyacrylamide Gel Environment and pollution Exact sciences and technology Food industries Fundamental and applied biological sciences. Psychology General aspects Hexose alkaline degradation products Humic substance Industrial applications and implications. Economical aspects Industrial wastewaters Kinetics Light Molasses - analysis Papain Papain - antagonists & inhibitors Pollution Pollution and sludges Proteinase inhibitor Reverse-phase chromatography Saccharum - chemistry Scattering, Radiation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Spectrophotometry, Infrared Spectrophotometry, Ultraviolet Stokes radius Wastewaters Water treatment and pollution |
| title | Separation and characterization of the colored material from sugarcane molasses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T04%3A44%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Separation%20and%20characterization%20of%20the%20colored%20material%20from%20sugarcane%20molasses&rft.jtitle=Chemosphere%20(Oxford)&rft.au=Hatano,%20Ken-ichi&rft.date=2008-04-01&rft.volume=71&rft.issue=9&rft.spage=1730&rft.epage=1737&rft.pages=1730-1737&rft.issn=0045-6535&rft.eissn=1879-1298&rft.coden=CMSHAF&rft_id=info:doi/10.1016/j.chemosphere.2007.12.019&rft_dat=%3Cproquest_cross%3E70502556%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70502556&rft_id=info:pmid/18267325&rft_els_id=S0045653507015445&rfr_iscdi=true |