Halomonas cerina sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium
Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain Correspondence Victoria Béjar vbejar{at}ugr.es Three bacterial strains were isolated from different saline soils in Spain. The nov...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2008-04, Vol.58 (4), p.803-809 |
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| creator | Gonzalez-Domenech, Carmen M Martinez-Checa, Fernando Quesada, Emilia Bejar, Victoria |
| description | Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain
Correspondence Victoria Béjar vbejar{at}ugr.es
Three bacterial strains were isolated from different saline soils in Spain. The novel strains were moderately halophilic, exopolysaccharide-producing, Gram-negative, non-motile rods. The strains required NaCl and grew best with 7.5–10 % (w/v) NaCl in the medium. They formed wax-coloured colonies, were oxidase-positive and showed respiratory metabolism, using oxygen, nitrate and nitrite as terminal electron acceptors. The novel strains were able to denitrify and did not produce acid from sugars. The DNA G+C contents varied between 62.7 and 66.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and sequence signatures of this gene showed that all three novel isolates belonged to the genus Halomonas in the class Gammaproteobacteria and formed an independent phylogenetic line. The most phylogenetically related species were Halomonas alimentaria , Halomonas campaniensis, Halomonas gudaonensis and Halomonas ventosae , with which the novel strains showed 16S rRNA gene sequence similarity values of between 96.3 and 95.2 %. The principal fatty acids of the novel strains were 16 : 0, 18 : 1 7 c , 16 : 1 7 c and 19 : 0 cyclo 8 c . The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q-9). The name Halomonas cerina sp. nov. is proposed for these isolates. The type strain is SP4 T (=CECT 7282 T =LMG 24145 T ).
Abbreviations: EPS, exopolysaccharide; PHA, poly- β -hydroxyalkanoate
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 15CR, SP4 T and R53 are EF613111 , EF613112 and EF613110 , respectively.
A dendrogram of phenotypic characteristics based on the simple-matching coefficient and UPGMA methods is available with the online version of this paper. |
| doi_str_mv | 10.1099/ijs.0.65322-0 |
| format | Article |
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Correspondence Victoria Béjar vbejar{at}ugr.es
Three bacterial strains were isolated from different saline soils in Spain. The novel strains were moderately halophilic, exopolysaccharide-producing, Gram-negative, non-motile rods. The strains required NaCl and grew best with 7.5–10 % (w/v) NaCl in the medium. They formed wax-coloured colonies, were oxidase-positive and showed respiratory metabolism, using oxygen, nitrate and nitrite as terminal electron acceptors. The novel strains were able to denitrify and did not produce acid from sugars. The DNA G+C contents varied between 62.7 and 66.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and sequence signatures of this gene showed that all three novel isolates belonged to the genus Halomonas in the class Gammaproteobacteria and formed an independent phylogenetic line. The most phylogenetically related species were Halomonas alimentaria , Halomonas campaniensis, Halomonas gudaonensis and Halomonas ventosae , with which the novel strains showed 16S rRNA gene sequence similarity values of between 96.3 and 95.2 %. The principal fatty acids of the novel strains were 16 : 0, 18 : 1 7 c , 16 : 1 7 c and 19 : 0 cyclo 8 c . The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q-9). The name Halomonas cerina sp. nov. is proposed for these isolates. The type strain is SP4 T (=CECT 7282 T =LMG 24145 T ).
Abbreviations: EPS, exopolysaccharide; PHA, poly- β -hydroxyalkanoate
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 15CR, SP4 T and R53 are EF613111 , EF613112 and EF613110 , respectively.
A dendrogram of phenotypic characteristics based on the simple-matching coefficient and UPGMA methods is available with the online version of this paper.</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.65322-0</identifier><identifier>PMID: 18398173</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Bacteria ; Bacteriology ; Base Composition ; Biological and medical sciences ; Carbohydrate Metabolism ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Halomonas ; Halomonas - classification ; Halomonas - genetics ; Halomonas - isolation & purification ; Halomonas - metabolism ; Microbiology ; Microscopy, Electron, Transmission ; Miscellaneous ; Molecular Sequence Data ; Phenotype ; Phylogeny ; Polysaccharides, Bacterial - biosynthesis ; RNA, Bacterial - genetics ; RNA, Ribosomal, 16S - genetics ; Soil Microbiology ; Spain ; Species Specificity ; Systematics ; Terminology as Topic</subject><ispartof>International journal of systematic and evolutionary microbiology, 2008-04, Vol.58 (4), p.803-809</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-9e9c32c24e318c2f3dd0e23044176a8e12c00d95a0572b95635db0d742f196703</citedby><cites>FETCH-LOGICAL-c423t-9e9c32c24e318c2f3dd0e23044176a8e12c00d95a0572b95635db0d742f196703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3733,3734,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20297254$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18398173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gonzalez-Domenech, Carmen M</creatorcontrib><creatorcontrib>Martinez-Checa, Fernando</creatorcontrib><creatorcontrib>Quesada, Emilia</creatorcontrib><creatorcontrib>Bejar, Victoria</creatorcontrib><title>Halomonas cerina sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain
Correspondence Victoria Béjar vbejar{at}ugr.es
Three bacterial strains were isolated from different saline soils in Spain. The novel strains were moderately halophilic, exopolysaccharide-producing, Gram-negative, non-motile rods. The strains required NaCl and grew best with 7.5–10 % (w/v) NaCl in the medium. They formed wax-coloured colonies, were oxidase-positive and showed respiratory metabolism, using oxygen, nitrate and nitrite as terminal electron acceptors. The novel strains were able to denitrify and did not produce acid from sugars. The DNA G+C contents varied between 62.7 and 66.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and sequence signatures of this gene showed that all three novel isolates belonged to the genus Halomonas in the class Gammaproteobacteria and formed an independent phylogenetic line. The most phylogenetically related species were Halomonas alimentaria , Halomonas campaniensis, Halomonas gudaonensis and Halomonas ventosae , with which the novel strains showed 16S rRNA gene sequence similarity values of between 96.3 and 95.2 %. The principal fatty acids of the novel strains were 16 : 0, 18 : 1 7 c , 16 : 1 7 c and 19 : 0 cyclo 8 c . The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q-9). The name Halomonas cerina sp. nov. is proposed for these isolates. The type strain is SP4 T (=CECT 7282 T =LMG 24145 T ).
Abbreviations: EPS, exopolysaccharide; PHA, poly- β -hydroxyalkanoate
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 15CR, SP4 T and R53 are EF613111 , EF613112 and EF613110 , respectively.
A dendrogram of phenotypic characteristics based on the simple-matching coefficient and UPGMA methods is available with the online version of this paper.</description><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Base Composition</subject><subject>Biological and medical sciences</subject><subject>Carbohydrate Metabolism</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Halomonas</subject><subject>Halomonas - classification</subject><subject>Halomonas - genetics</subject><subject>Halomonas - isolation & purification</subject><subject>Halomonas - metabolism</subject><subject>Microbiology</subject><subject>Microscopy, Electron, Transmission</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Phenotype</subject><subject>Phylogeny</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Soil Microbiology</subject><subject>Spain</subject><subject>Species Specificity</subject><subject>Systematics</subject><subject>Terminology as Topic</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c2P1CAYBnBiNO66evRquKiXaX35KuVoNruuySZe9Ewo0CmbtlRoV-e_l3Em69ETBH55eMKL0FsCNQGlPoWHXEPdCEZpBc_QJeFNUwlg_PnTnjYX6FXODwDlAOAluiAtUy2R7BLt78wYpzibjK1PYTY4LzWe42O9wwZP0flkVj8e8FDcMoQx2B12fg5rCv0hzPsd9r_jEsdDNtYOJgXnqyVFt9lyiTtj1xK7Ta_Ri96M2b85r1fox-3N9-u76v7bl6_Xn-8ryylbK-WVZdRS7hlpLe2Zc-ApA86JbEzrCbUATgkDQtJOiYYJ14GTnPZENRLYFfpwyi0dfm4-r3oK2fpxNLOPW9YSuKJSif9CoiQvsW2B1QnaFHNOvtdLCpNJB01AH0egywg06L8j0McG787BWzd590-f_7yA92dgsjVjn8xsQ35yFKiSVPDiPp7cEPbDr5C83vt5CqVGF-LxUdFqrltg7A_Y3Zzx</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Gonzalez-Domenech, Carmen M</creator><creator>Martinez-Checa, Fernando</creator><creator>Quesada, Emilia</creator><creator>Bejar, Victoria</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080401</creationdate><title>Halomonas cerina sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium</title><author>Gonzalez-Domenech, Carmen M ; Martinez-Checa, Fernando ; Quesada, Emilia ; Bejar, Victoria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-9e9c32c24e318c2f3dd0e23044176a8e12c00d95a0572b95635db0d742f196703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Base Composition</topic><topic>Biological and medical sciences</topic><topic>Carbohydrate Metabolism</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Halomonas</topic><topic>Halomonas - classification</topic><topic>Halomonas - genetics</topic><topic>Halomonas - isolation & purification</topic><topic>Halomonas - metabolism</topic><topic>Microbiology</topic><topic>Microscopy, Electron, Transmission</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Phenotype</topic><topic>Phylogeny</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Soil Microbiology</topic><topic>Spain</topic><topic>Species Specificity</topic><topic>Systematics</topic><topic>Terminology as Topic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gonzalez-Domenech, Carmen M</creatorcontrib><creatorcontrib>Martinez-Checa, Fernando</creatorcontrib><creatorcontrib>Quesada, Emilia</creatorcontrib><creatorcontrib>Bejar, Victoria</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gonzalez-Domenech, Carmen M</au><au>Martinez-Checa, Fernando</au><au>Quesada, Emilia</au><au>Bejar, Victoria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Halomonas cerina sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>58</volume><issue>4</issue><spage>803</spage><epage>809</epage><pages>803-809</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, University of Granada, 18071 Granada, Spain
Correspondence Victoria Béjar vbejar{at}ugr.es
Three bacterial strains were isolated from different saline soils in Spain. The novel strains were moderately halophilic, exopolysaccharide-producing, Gram-negative, non-motile rods. The strains required NaCl and grew best with 7.5–10 % (w/v) NaCl in the medium. They formed wax-coloured colonies, were oxidase-positive and showed respiratory metabolism, using oxygen, nitrate and nitrite as terminal electron acceptors. The novel strains were able to denitrify and did not produce acid from sugars. The DNA G+C contents varied between 62.7 and 66.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and sequence signatures of this gene showed that all three novel isolates belonged to the genus Halomonas in the class Gammaproteobacteria and formed an independent phylogenetic line. The most phylogenetically related species were Halomonas alimentaria , Halomonas campaniensis, Halomonas gudaonensis and Halomonas ventosae , with which the novel strains showed 16S rRNA gene sequence similarity values of between 96.3 and 95.2 %. The principal fatty acids of the novel strains were 16 : 0, 18 : 1 7 c , 16 : 1 7 c and 19 : 0 cyclo 8 c . The predominant respiratory lipoquinone was ubiquinone with nine isoprene units (Q-9). The name Halomonas cerina sp. nov. is proposed for these isolates. The type strain is SP4 T (=CECT 7282 T =LMG 24145 T ).
Abbreviations: EPS, exopolysaccharide; PHA, poly- β -hydroxyalkanoate
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 15CR, SP4 T and R53 are EF613111 , EF613112 and EF613110 , respectively.
A dendrogram of phenotypic characteristics based on the simple-matching coefficient and UPGMA methods is available with the online version of this paper.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>18398173</pmid><doi>10.1099/ijs.0.65322-0</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacteria Bacteriology Base Composition Biological and medical sciences Carbohydrate Metabolism DNA, Bacterial - chemistry DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genes, Bacterial Halomonas Halomonas - classification Halomonas - genetics Halomonas - isolation & purification Halomonas - metabolism Microbiology Microscopy, Electron, Transmission Miscellaneous Molecular Sequence Data Phenotype Phylogeny Polysaccharides, Bacterial - biosynthesis RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Soil Microbiology Spain Species Specificity Systematics Terminology as Topic |
| title | Halomonas cerina sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium |
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