ATP promotes NCX-reversal in aortic smooth muscle cells by DAG-activated Na + entry
Reversal of the plasma membrane Na +/Ca 2+ exchanger (NCX) has been shown to mediate Ca 2+ influx in response to activation of G-protein linked receptors. Functional coupling of reverse-mode NCX with canonical transient receptor potential channels (TRPC), specifically TRPC6, has recently been demons...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 2007-06, Vol.357 (4), p.1177-1182 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1182 |
|---|---|
| container_issue | 4 |
| container_start_page | 1177 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 357 |
| creator | Syyong, Harley T. Poburko, Damon Fameli, Nicola Breemen, Cornelis van |
| description | Reversal of the plasma membrane Na
+/Ca
2+ exchanger (NCX) has been shown to mediate Ca
2+ influx in response to activation of G-protein linked receptors. Functional coupling of reverse-mode NCX with canonical transient receptor potential channels (TRPC), specifically TRPC6, has recently been demonstrated by our laboratory to mediate Ca
2+ influx in rat aortic smooth muscle cells (RASMCs) following ATP stimulation. In this communication, we provide further detail of this functional coupling by indirectly measuring NCX reversal. We found that NCX reversal, induced by the removal of extracellular Na
+, was increased following stimulation with ATP and the diacylglycerol analog 1-Oleoyl-2-acetyl-
sn-glycerol. This increased NCX reversal was attenuated by SKF-96365, an inhibitor of non-selective cation channels, and by activation of protein kinase C with phorbol ester 12-tetradecanoylphorbol-13 acetate. These data are consistent with the known properties of TRPC6 and further support that functional coupling of TRPC6 and NCX occurs via a receptor-operated, rather than store-operated, cascade in RASMCs. |
| doi_str_mv | 10.1016/j.bbrc.2007.04.080 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70488449</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X07008169</els_id><sourcerecordid>70488449</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354t-c3667ea92232a33857a11a64a38e5f30eb9d09ea6931cc788ce50dbd5978964e3</originalsourceid><addsrcrecordid>eNp9kEFLwzAUx4Mobk6_gAfJyYu0vrRp2oCXMXUKYwpO2C2k6RtmtKsm2WDf3pYNvHl57_L7_3nvR8g1g5gBE_fruCydiROAPAYeQwEnZMhAQpQw4KdkCAAiSiRbDsiF92sAxriQ52TAci5EksOQfIwX7_TbtU0b0NP5ZBk53KHzuqZ2Q3XrgjXUN20bvmiz9aZGarCuPS339HE8jbQJdqcDVnSu6R3FTXD7S3K20rXHq-Mekc_np8XkJZq9TV8n41lk0oyHbgqRo5ZJkiY6TYss14xpwXVaYLZKAUtZgUQtZMqMyYvCYAZVWWUyL6TgmI7I7aG3u_9niz6oxvr-Or3BdutVDrwoOJcdmBxA41rvHa7Ut7ONdnvFQPUq1Vr1KlWvUgFXncoudHNs35YNVn-Ro7sOeDgA2P24s-iUNxY3Bivr0ARVtfa__l8LXINj</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70488449</pqid></control><display><type>article</type><title>ATP promotes NCX-reversal in aortic smooth muscle cells by DAG-activated Na + entry</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Syyong, Harley T. ; Poburko, Damon ; Fameli, Nicola ; Breemen, Cornelis van</creator><creatorcontrib>Syyong, Harley T. ; Poburko, Damon ; Fameli, Nicola ; Breemen, Cornelis van</creatorcontrib><description>Reversal of the plasma membrane Na
+/Ca
2+ exchanger (NCX) has been shown to mediate Ca
2+ influx in response to activation of G-protein linked receptors. Functional coupling of reverse-mode NCX with canonical transient receptor potential channels (TRPC), specifically TRPC6, has recently been demonstrated by our laboratory to mediate Ca
2+ influx in rat aortic smooth muscle cells (RASMCs) following ATP stimulation. In this communication, we provide further detail of this functional coupling by indirectly measuring NCX reversal. We found that NCX reversal, induced by the removal of extracellular Na
+, was increased following stimulation with ATP and the diacylglycerol analog 1-Oleoyl-2-acetyl-
sn-glycerol. This increased NCX reversal was attenuated by SKF-96365, an inhibitor of non-selective cation channels, and by activation of protein kinase C with phorbol ester 12-tetradecanoylphorbol-13 acetate. These data are consistent with the known properties of TRPC6 and further support that functional coupling of TRPC6 and NCX occurs via a receptor-operated, rather than store-operated, cascade in RASMCs.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2007.04.080</identifier><identifier>PMID: 17466270</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenosine Triphosphate - administration & dosage ; Aequorin ; Animals ; Aorta - drug effects ; Aorta - metabolism ; Calcium - metabolism ; Cells, Cultured ; Dose-Response Relationship, Drug ; Microdomains ; Mitochondria ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Myocytes, Smooth Muscle - drug effects ; Myocytes, Smooth Muscle - metabolism ; Na + entry ; Na +/Ca 2+ exchanger ; Rats ; Sarcoplasmic reticulum ; Smooth muscle cells ; Sodium - metabolism ; Sodium-Calcium Exchanger - metabolism ; TRPC6</subject><ispartof>Biochemical and biophysical research communications, 2007-06, Vol.357 (4), p.1177-1182</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-c3667ea92232a33857a11a64a38e5f30eb9d09ea6931cc788ce50dbd5978964e3</citedby><cites>FETCH-LOGICAL-c354t-c3667ea92232a33857a11a64a38e5f30eb9d09ea6931cc788ce50dbd5978964e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2007.04.080$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17466270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Syyong, Harley T.</creatorcontrib><creatorcontrib>Poburko, Damon</creatorcontrib><creatorcontrib>Fameli, Nicola</creatorcontrib><creatorcontrib>Breemen, Cornelis van</creatorcontrib><title>ATP promotes NCX-reversal in aortic smooth muscle cells by DAG-activated Na + entry</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Reversal of the plasma membrane Na
+/Ca
2+ exchanger (NCX) has been shown to mediate Ca
2+ influx in response to activation of G-protein linked receptors. Functional coupling of reverse-mode NCX with canonical transient receptor potential channels (TRPC), specifically TRPC6, has recently been demonstrated by our laboratory to mediate Ca
2+ influx in rat aortic smooth muscle cells (RASMCs) following ATP stimulation. In this communication, we provide further detail of this functional coupling by indirectly measuring NCX reversal. We found that NCX reversal, induced by the removal of extracellular Na
+, was increased following stimulation with ATP and the diacylglycerol analog 1-Oleoyl-2-acetyl-
sn-glycerol. This increased NCX reversal was attenuated by SKF-96365, an inhibitor of non-selective cation channels, and by activation of protein kinase C with phorbol ester 12-tetradecanoylphorbol-13 acetate. These data are consistent with the known properties of TRPC6 and further support that functional coupling of TRPC6 and NCX occurs via a receptor-operated, rather than store-operated, cascade in RASMCs.</description><subject>Adenosine Triphosphate - administration & dosage</subject><subject>Aequorin</subject><subject>Animals</subject><subject>Aorta - drug effects</subject><subject>Aorta - metabolism</subject><subject>Calcium - metabolism</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Microdomains</subject><subject>Mitochondria</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Myocytes, Smooth Muscle - drug effects</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Na + entry</subject><subject>Na +/Ca 2+ exchanger</subject><subject>Rats</subject><subject>Sarcoplasmic reticulum</subject><subject>Smooth muscle cells</subject><subject>Sodium - metabolism</subject><subject>Sodium-Calcium Exchanger - metabolism</subject><subject>TRPC6</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFLwzAUx4Mobk6_gAfJyYu0vrRp2oCXMXUKYwpO2C2k6RtmtKsm2WDf3pYNvHl57_L7_3nvR8g1g5gBE_fruCydiROAPAYeQwEnZMhAQpQw4KdkCAAiSiRbDsiF92sAxriQ52TAci5EksOQfIwX7_TbtU0b0NP5ZBk53KHzuqZ2Q3XrgjXUN20bvmiz9aZGarCuPS339HE8jbQJdqcDVnSu6R3FTXD7S3K20rXHq-Mekc_np8XkJZq9TV8n41lk0oyHbgqRo5ZJkiY6TYss14xpwXVaYLZKAUtZgUQtZMqMyYvCYAZVWWUyL6TgmI7I7aG3u_9niz6oxvr-Or3BdutVDrwoOJcdmBxA41rvHa7Ut7ONdnvFQPUq1Vr1KlWvUgFXncoudHNs35YNVn-Ro7sOeDgA2P24s-iUNxY3Bivr0ARVtfa__l8LXINj</recordid><startdate>20070615</startdate><enddate>20070615</enddate><creator>Syyong, Harley T.</creator><creator>Poburko, Damon</creator><creator>Fameli, Nicola</creator><creator>Breemen, Cornelis van</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070615</creationdate><title>ATP promotes NCX-reversal in aortic smooth muscle cells by DAG-activated Na + entry</title><author>Syyong, Harley T. ; Poburko, Damon ; Fameli, Nicola ; Breemen, Cornelis van</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-c3667ea92232a33857a11a64a38e5f30eb9d09ea6931cc788ce50dbd5978964e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenosine Triphosphate - administration & dosage</topic><topic>Aequorin</topic><topic>Animals</topic><topic>Aorta - drug effects</topic><topic>Aorta - metabolism</topic><topic>Calcium - metabolism</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Microdomains</topic><topic>Mitochondria</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Myocytes, Smooth Muscle - drug effects</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Na + entry</topic><topic>Na +/Ca 2+ exchanger</topic><topic>Rats</topic><topic>Sarcoplasmic reticulum</topic><topic>Smooth muscle cells</topic><topic>Sodium - metabolism</topic><topic>Sodium-Calcium Exchanger - metabolism</topic><topic>TRPC6</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Syyong, Harley T.</creatorcontrib><creatorcontrib>Poburko, Damon</creatorcontrib><creatorcontrib>Fameli, Nicola</creatorcontrib><creatorcontrib>Breemen, Cornelis van</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Syyong, Harley T.</au><au>Poburko, Damon</au><au>Fameli, Nicola</au><au>Breemen, Cornelis van</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ATP promotes NCX-reversal in aortic smooth muscle cells by DAG-activated Na + entry</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-06-15</date><risdate>2007</risdate><volume>357</volume><issue>4</issue><spage>1177</spage><epage>1182</epage><pages>1177-1182</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Reversal of the plasma membrane Na
+/Ca
2+ exchanger (NCX) has been shown to mediate Ca
2+ influx in response to activation of G-protein linked receptors. Functional coupling of reverse-mode NCX with canonical transient receptor potential channels (TRPC), specifically TRPC6, has recently been demonstrated by our laboratory to mediate Ca
2+ influx in rat aortic smooth muscle cells (RASMCs) following ATP stimulation. In this communication, we provide further detail of this functional coupling by indirectly measuring NCX reversal. We found that NCX reversal, induced by the removal of extracellular Na
+, was increased following stimulation with ATP and the diacylglycerol analog 1-Oleoyl-2-acetyl-
sn-glycerol. This increased NCX reversal was attenuated by SKF-96365, an inhibitor of non-selective cation channels, and by activation of protein kinase C with phorbol ester 12-tetradecanoylphorbol-13 acetate. These data are consistent with the known properties of TRPC6 and further support that functional coupling of TRPC6 and NCX occurs via a receptor-operated, rather than store-operated, cascade in RASMCs.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17466270</pmid><doi>10.1016/j.bbrc.2007.04.080</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 2007-06, Vol.357 (4), p.1177-1182 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70488449 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Triphosphate - administration & dosage Aequorin Animals Aorta - drug effects Aorta - metabolism Calcium - metabolism Cells, Cultured Dose-Response Relationship, Drug Microdomains Mitochondria Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Myocytes, Smooth Muscle - drug effects Myocytes, Smooth Muscle - metabolism Na + entry Na +/Ca 2+ exchanger Rats Sarcoplasmic reticulum Smooth muscle cells Sodium - metabolism Sodium-Calcium Exchanger - metabolism TRPC6 |
| title | ATP promotes NCX-reversal in aortic smooth muscle cells by DAG-activated Na + entry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T04%3A16%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=ATP%20promotes%20NCX-reversal%20in%20aortic%20smooth%20muscle%20cells%20by%20DAG-activated%20Na%20+%20entry&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Syyong,%20Harley%20T.&rft.date=2007-06-15&rft.volume=357&rft.issue=4&rft.spage=1177&rft.epage=1182&rft.pages=1177-1182&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1016/j.bbrc.2007.04.080&rft_dat=%3Cproquest_cross%3E70488449%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70488449&rft_id=info:pmid/17466270&rft_els_id=S0006291X07008169&rfr_iscdi=true |