Glucagon receptor expression and glucagon stimulation of ghrelin secretion in rat stomach
The present study was performed to evaluate the role of glucagon in the regulation of ghrelin secretion from the rat stomach. mRNA for ghrelin and glucagon receptor was expressed predominantly in the lower body and pylorus of stomach, but little or not in the upper body and cardia. Ghrelin- and gluc...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2007-06, Vol.357 (4), p.865-870 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Katayama, Tetsuro Shimamoto, Shohei Oda, Hideyuki Nakahara, Keiko Kangawa, Kenji Murakami, Noboru |
| description | The present study was performed to evaluate the role of glucagon in the regulation of ghrelin secretion from the rat stomach. mRNA for ghrelin and glucagon receptor was expressed predominantly in the lower body and pylorus of stomach, but little or not in the upper body and cardia. Ghrelin- and glucagon receptor-immunoreactive cells were detected in lamina propria mucosae of stomach and some cells expressed both. Intravenous administration of glucagon caused transient increases in both acyl- and desacyl-ghrelin levels in the gastric vein within 10
min, which was followed by gradual increases in desacyl-ghrelin levels until 60
min. Steady state levels of ghrelin mRNA in the stomach were increased by 1.9-fold 20
min after glucagon administration, but not at 5 or 120
min. These results suggest that glucagon stimulates acute release of both forms of ghrelin and thereafter upregulates synthesis and release of desacyl-ghrelin in the rat stomach. |
| doi_str_mv | 10.1016/j.bbrc.2007.04.001 |
| format | Article |
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min, which was followed by gradual increases in desacyl-ghrelin levels until 60
min. Steady state levels of ghrelin mRNA in the stomach were increased by 1.9-fold 20
min after glucagon administration, but not at 5 or 120
min. These results suggest that glucagon stimulates acute release of both forms of ghrelin and thereafter upregulates synthesis and release of desacyl-ghrelin in the rat stomach.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2007.04.001</identifier><identifier>PMID: 17462598</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Desacyl-ghrelin ; Dose-Response Relationship, Drug ; Double staining ; Ghrelin ; Glucagon - administration & dosage ; Glucagon receptor ; Male ; Peptide Hormones - metabolism ; Quantitative RT-PCR ; Rat ; Rats ; Rats, Sprague-Dawley ; Receptors, Glucagon - metabolism ; Stomach ; Stomach - drug effects ; Stomach - metabolism ; Tissue Distribution ; Up-Regulation - drug effects ; Up-Regulation - physiology</subject><ispartof>Biochemical and biophysical research communications, 2007-06, Vol.357 (4), p.865-870</ispartof><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-bc8dd9825151a21ecabbe95acf881cbd5efbe3f44674fb04ea83a8dc5e4c40e93</citedby><cites>FETCH-LOGICAL-c464t-bc8dd9825151a21ecabbe95acf881cbd5efbe3f44674fb04ea83a8dc5e4c40e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bbrc.2007.04.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17462598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Katayama, Tetsuro</creatorcontrib><creatorcontrib>Shimamoto, Shohei</creatorcontrib><creatorcontrib>Oda, Hideyuki</creatorcontrib><creatorcontrib>Nakahara, Keiko</creatorcontrib><creatorcontrib>Kangawa, Kenji</creatorcontrib><creatorcontrib>Murakami, Noboru</creatorcontrib><title>Glucagon receptor expression and glucagon stimulation of ghrelin secretion in rat stomach</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The present study was performed to evaluate the role of glucagon in the regulation of ghrelin secretion from the rat stomach. mRNA for ghrelin and glucagon receptor was expressed predominantly in the lower body and pylorus of stomach, but little or not in the upper body and cardia. Ghrelin- and glucagon receptor-immunoreactive cells were detected in lamina propria mucosae of stomach and some cells expressed both. Intravenous administration of glucagon caused transient increases in both acyl- and desacyl-ghrelin levels in the gastric vein within 10
min, which was followed by gradual increases in desacyl-ghrelin levels until 60
min. Steady state levels of ghrelin mRNA in the stomach were increased by 1.9-fold 20
min after glucagon administration, but not at 5 or 120
min. These results suggest that glucagon stimulates acute release of both forms of ghrelin and thereafter upregulates synthesis and release of desacyl-ghrelin in the rat stomach.</description><subject>Animals</subject><subject>Desacyl-ghrelin</subject><subject>Dose-Response Relationship, Drug</subject><subject>Double staining</subject><subject>Ghrelin</subject><subject>Glucagon - administration & dosage</subject><subject>Glucagon receptor</subject><subject>Male</subject><subject>Peptide Hormones - metabolism</subject><subject>Quantitative RT-PCR</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Glucagon - metabolism</subject><subject>Stomach</subject><subject>Stomach - drug effects</subject><subject>Stomach - metabolism</subject><subject>Tissue Distribution</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - physiology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMo7rr6BzxIT95aJ920TcGLiK7CghcFPYV8THez9GNNWtF_b-quePM0zJtnXshDyDmFhALNrzaJUk4nKUCRAEsA6AGZUighTimwQzIFgDxOS_o6ISfebwJAWV4ekwktWJ5mJZ-St0U9aLnq2sihxm3fuQg_tw69tyGTrYlWv4DvbTPUsh8fuiparR3WNsSoHf6EYXGyD1zXSL0-JUeVrD2e7eeMvNzfPd8-xMunxePtzTLWLGd9rDQ3puRpRjMqU4paKoVlJnXFOdXKZFgpnFeM5QWrFDCUfC650RkyzQDL-Yxc7nq3rnsf0PeisV5jXcsWu8GLAhgvsjwLYLoDteu8d1iJrbONdF-CghiFio0YhYpRqAAmgq9wdLFvH1SD5u9kbzAA1zsAwx8_LDrhtcVWo7HBaC9MZ__r_wZ8v4nO</recordid><startdate>20070615</startdate><enddate>20070615</enddate><creator>Katayama, Tetsuro</creator><creator>Shimamoto, Shohei</creator><creator>Oda, Hideyuki</creator><creator>Nakahara, Keiko</creator><creator>Kangawa, Kenji</creator><creator>Murakami, Noboru</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070615</creationdate><title>Glucagon receptor expression and glucagon stimulation of ghrelin secretion in rat stomach</title><author>Katayama, Tetsuro ; Shimamoto, Shohei ; Oda, Hideyuki ; Nakahara, Keiko ; Kangawa, Kenji ; Murakami, Noboru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-bc8dd9825151a21ecabbe95acf881cbd5efbe3f44674fb04ea83a8dc5e4c40e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Desacyl-ghrelin</topic><topic>Dose-Response Relationship, Drug</topic><topic>Double staining</topic><topic>Ghrelin</topic><topic>Glucagon - administration & dosage</topic><topic>Glucagon receptor</topic><topic>Male</topic><topic>Peptide Hormones - metabolism</topic><topic>Quantitative RT-PCR</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Glucagon - metabolism</topic><topic>Stomach</topic><topic>Stomach - drug effects</topic><topic>Stomach - metabolism</topic><topic>Tissue Distribution</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Katayama, Tetsuro</creatorcontrib><creatorcontrib>Shimamoto, Shohei</creatorcontrib><creatorcontrib>Oda, Hideyuki</creatorcontrib><creatorcontrib>Nakahara, Keiko</creatorcontrib><creatorcontrib>Kangawa, Kenji</creatorcontrib><creatorcontrib>Murakami, Noboru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Katayama, Tetsuro</au><au>Shimamoto, Shohei</au><au>Oda, Hideyuki</au><au>Nakahara, Keiko</au><au>Kangawa, Kenji</au><au>Murakami, Noboru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glucagon receptor expression and glucagon stimulation of ghrelin secretion in rat stomach</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2007-06-15</date><risdate>2007</risdate><volume>357</volume><issue>4</issue><spage>865</spage><epage>870</epage><pages>865-870</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The present study was performed to evaluate the role of glucagon in the regulation of ghrelin secretion from the rat stomach. mRNA for ghrelin and glucagon receptor was expressed predominantly in the lower body and pylorus of stomach, but little or not in the upper body and cardia. Ghrelin- and glucagon receptor-immunoreactive cells were detected in lamina propria mucosae of stomach and some cells expressed both. Intravenous administration of glucagon caused transient increases in both acyl- and desacyl-ghrelin levels in the gastric vein within 10
min, which was followed by gradual increases in desacyl-ghrelin levels until 60
min. Steady state levels of ghrelin mRNA in the stomach were increased by 1.9-fold 20
min after glucagon administration, but not at 5 or 120
min. These results suggest that glucagon stimulates acute release of both forms of ghrelin and thereafter upregulates synthesis and release of desacyl-ghrelin in the rat stomach.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17462598</pmid><doi>10.1016/j.bbrc.2007.04.001</doi><tpages>6</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Desacyl-ghrelin Dose-Response Relationship, Drug Double staining Ghrelin Glucagon - administration & dosage Glucagon receptor Male Peptide Hormones - metabolism Quantitative RT-PCR Rat Rats Rats, Sprague-Dawley Receptors, Glucagon - metabolism Stomach Stomach - drug effects Stomach - metabolism Tissue Distribution Up-Regulation - drug effects Up-Regulation - physiology |
| title | Glucagon receptor expression and glucagon stimulation of ghrelin secretion in rat stomach |
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