HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure
An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (...
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| Veröffentlicht in: | Journal of separation science 2008-04, Vol.31 (6-7), p.1007-1014 |
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| container_title | Journal of separation science |
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| creator | Mercolini, Laura Addolorata Saracino, Maria Bugamelli, Francesca Ferranti, Anna Malaguti, Marco Hrelia, Silvana Raggi, Maria Augusta |
| description | An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mmx4.6 mm id, 5 μm) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at λ = 386 nm while exciting at λ = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03-5.00 ng/mL for MLT, 3-500 ng/mL for t-RSV and 1-150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated. |
| doi_str_mv | 10.1002/jssc.200700458 |
| format | Article |
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The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mmx4.6 mm id, 5 μm) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at λ = 386 nm while exciting at λ = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03-5.00 ng/mL for MLT, 3-500 ng/mL for t-RSV and 1-150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.200700458</identifier><identifier>PMID: 18338365</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>Antioxidant activity ; Antioxidants - analysis ; Antioxidants - chemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Fermented food industries ; Flavonoids - analysis ; Flavonoids - chemistry ; Food industries ; Fundamental and applied biological sciences. Psychology ; Isomerism ; Liquid chromatography with fluorescence detection ; Melatonin ; Melatonin - analysis ; Melatonin - chemistry ; Molecular Structure ; Phenols - analysis ; Phenols - chemistry ; Polyphenols ; Resveratrol isomers ; Solid Phase Extraction - methods ; Stilbenes - analysis ; Stilbenes - chemistry ; Wine ; Wine - analysis ; Wines and vinegars</subject><ispartof>Journal of separation science, 2008-04, Vol.31 (6-7), p.1007-1014</ispartof><rights>Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. 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Sep. Science</addtitle><description>An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mmx4.6 mm id, 5 μm) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at λ = 386 nm while exciting at λ = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03-5.00 ng/mL for MLT, 3-500 ng/mL for t-RSV and 1-150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.</description><subject>Antioxidant activity</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - chemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fermented food industries</subject><subject>Flavonoids - analysis</subject><subject>Flavonoids - chemistry</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isomerism</subject><subject>Liquid chromatography with fluorescence detection</subject><subject>Melatonin</subject><subject>Melatonin - analysis</subject><subject>Melatonin - chemistry</subject><subject>Molecular Structure</subject><subject>Phenols - analysis</subject><subject>Phenols - chemistry</subject><subject>Polyphenols</subject><subject>Resveratrol isomers</subject><subject>Solid Phase Extraction - methods</subject><subject>Stilbenes - analysis</subject><subject>Stilbenes - chemistry</subject><subject>Wine</subject><subject>Wine - analysis</subject><subject>Wines and vinegars</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Mtv1DAQB-AIgWgpXDlCLnDLdmwn9uSIVn1QbcuiUMHNcpxJ5ZJHsTeU_e_xKquFW0-27G8e-iXJWwYLBsBP70OwCw6gAPICnyXHTLIiKwXLnx_uII-SVyHcAzCFJbxMjhgKgUIWx0l1uV4ts_PUDKbbBhfSsU176sxmHNwQX5vUU_hN3mz82KUujD35kMavRzdQOgU33EWVVuuz9MGPlprJ0-vkRWu6QG_250lye372bXmZrb5cfF5-WmVWIMOsRclN09ZNWRZoqawRGTW2ri2TJBVhU0ZiyEYhBZMcTG0V1shkDkrW4iT5OPeNk39NFDa6d8FS15mBxiloBTkKBPUkFBxzJiREuJih9WMInlr94F1v_FYz0Lu89S5vfcg7Frzbd57qnpp_fB9wBB_2wARrutabwbpwcBw48lLtGpWze3QdbZ8Yq6-qavn_Etlc68KG_hxqjf-ppRKq0N9vLvTV9Q-Ja3mtv0b_fvatGbW583Gf24oDEwCIOS-F-AtoUrHu</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Mercolini, Laura</creator><creator>Addolorata Saracino, Maria</creator><creator>Bugamelli, Francesca</creator><creator>Ferranti, Anna</creator><creator>Malaguti, Marco</creator><creator>Hrelia, Silvana</creator><creator>Raggi, Maria Augusta</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure</title><author>Mercolini, Laura ; Addolorata Saracino, Maria ; Bugamelli, Francesca ; Ferranti, Anna ; Malaguti, Marco ; Hrelia, Silvana ; Raggi, Maria Augusta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3818-f862adfbd9958ce9b881edcbbc16e67e8d9f86aecbd9631620abc78b8164076b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antioxidant activity</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - chemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fermented food industries</topic><topic>Flavonoids - analysis</topic><topic>Flavonoids - chemistry</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isomerism</topic><topic>Liquid chromatography with fluorescence detection</topic><topic>Melatonin</topic><topic>Melatonin - analysis</topic><topic>Melatonin - chemistry</topic><topic>Molecular Structure</topic><topic>Phenols - analysis</topic><topic>Phenols - chemistry</topic><topic>Polyphenols</topic><topic>Resveratrol isomers</topic><topic>Solid Phase Extraction - methods</topic><topic>Stilbenes - analysis</topic><topic>Stilbenes - chemistry</topic><topic>Wine</topic><topic>Wine - analysis</topic><topic>Wines and vinegars</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mercolini, Laura</creatorcontrib><creatorcontrib>Addolorata Saracino, Maria</creatorcontrib><creatorcontrib>Bugamelli, Francesca</creatorcontrib><creatorcontrib>Ferranti, Anna</creatorcontrib><creatorcontrib>Malaguti, Marco</creatorcontrib><creatorcontrib>Hrelia, Silvana</creatorcontrib><creatorcontrib>Raggi, Maria Augusta</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mercolini, Laura</au><au>Addolorata Saracino, Maria</au><au>Bugamelli, Francesca</au><au>Ferranti, Anna</au><au>Malaguti, Marco</au><au>Hrelia, Silvana</au><au>Raggi, Maria Augusta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2008-04</date><risdate>2008</risdate><volume>31</volume><issue>6-7</issue><spage>1007</spage><epage>1014</epage><pages>1007-1014</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>An original analytical method has been developed for the determination of the antioxidants trans-resveratrol (t-RSV) and cis-resveratrol (c-RSV) and of melatonin (MLT) in red and white wine. The method is based on HPLC coupled to fluorescence detection. Separation was obtained by using a RP column (C8, 150 mmx4.6 mm id, 5 μm) and a mobile phase composed of 79% aqueous phosphate buffer at pH 3.0 and 21% ACN. Fluorescence intensity was monitored at λ = 386 nm while exciting at λ = 298 nm, mirtazapine was used as the internal standard. A careful pretreatment of wine samples was developed, using SPE with C18 cartridges (100 mg, 1 mL). The calibration curves were linear over the following concentration ranges: 0.03-5.00 ng/mL for MLT, 3-500 ng/mL for t-RSV and 1-150 ng/mL for c-RSV. The LOD values were 0.01 ng/mL for MLT, 1 ng/mL for t-RSV and 0.3 ng/mL for c-RSV. Precision data, as well as extraction yield and sample purification results, were satisfactory. Thus, the method seems to be suitable for the analysis of MLT and resveratrol isomers in wine samples. Moreover, wine total polyphenol content and antioxidant activity were evaluated.</abstract><cop>Weinheim</cop><pub>Wiley-VCH Verlag</pub><pmid>18338365</pmid><doi>10.1002/jssc.200700458</doi><tpages>8</tpages></addata></record> |
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| subjects | Antioxidant activity Antioxidants - analysis Antioxidants - chemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Fermented food industries Flavonoids - analysis Flavonoids - chemistry Food industries Fundamental and applied biological sciences. Psychology Isomerism Liquid chromatography with fluorescence detection Melatonin Melatonin - analysis Melatonin - chemistry Molecular Structure Phenols - analysis Phenols - chemistry Polyphenols Resveratrol isomers Solid Phase Extraction - methods Stilbenes - analysis Stilbenes - chemistry Wine Wine - analysis Wines and vinegars |
| title | HPLC-F analysis of melatonin and resveratrol isomers in wine using an SPE procedure |
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