Tissue bioengineering for surgical bleb defects: an animal study
Background To assess the effectiveness of OloGen (also named iGen), a porous, bioengineered, biodegradable, collagen-glycoaminoglycan matrix implant, in preventing poor bleb formation and early failure after trabeculectomy in eyes with a surgical wound defect. Methods The right eyes of 30 female New...
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| Veröffentlicht in: | Graefe's archive for clinical and experimental ophthalmology 2008-05, Vol.246 (5), p.709-717 |
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| creator | Hsu, Wei-Cherng Ritch, Robert Krupin, Theodore Chen, Henry Shen-Lih |
| description | Background
To assess the effectiveness of OloGen (also named iGen), a porous, bioengineered, biodegradable, collagen-glycoaminoglycan matrix implant, in preventing poor bleb formation and early failure after trabeculectomy in eyes with a surgical wound defect.
Methods
The right eyes of 30 female New Zealand albino rabbits underwent trabeculectomy with OloGen implanted subconjunctivally on top of the scleral flap, while six right eyes received trabeculectomy without the implant to serve as a control group. A 1–2 mm diameter circular conjunctival defect was created in all eyes. Six rabbits in the group receiving the implant were sacrificed on days 3, 5, 7, 21, and 28. Rabbits in the control group were sacrificed on day 28. Perkins applanation tonometry, Seidel test and measurement of both the extent of the conjunctival defect and the anterior chamber depth were performed. Enucleated eyes were fixed in 4% formaldehyde and stained with hematoxylin and eosin (H&E) for general histological observation, and with Sirius and Fast-green stains to assess collagen deposition and cell migration.
Results
Seidel tests were negative for all operated and control eyes. No flat anterior chamber occurred in either group. With the exception of days 5 and 7, post-operative mean IOP difference is significant in both groups, (
P
> 0.05 for day 5, 7 and
P
|
| doi_str_mv | 10.1007/s00417-007-0744-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70482412</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70482412</sourcerecordid><originalsourceid>FETCH-LOGICAL-c369t-75121a2fdb6f7fd79a95fa63083e63896971fb04bc7b1a61b7573789f23b5dcb3</originalsourceid><addsrcrecordid>eNp1kM9LwzAUx4MoOqd_gBcpHrxF85ImaTwpw18w8DJht5C0yejo2pm0h_33ZnQwEIRAHo_P-ybvg9ANkAcgRD5GQnKQOJWYyDzH6gRNIGccS0KXp2hCJAVcMLq8QJcxrknCGYdzdAEFFVwpOUHPizrGwWW27ly7qlvnQt2uMt-FLA5hVZemyWzjbFY578o-PmWmTafepH7sh2p3hc68aaK7PtxT9P32uph94PnX--fsZY5LJlSPJQcKhvrKCi99JZVR3BvBSMGcYIUSSoK3JLeltGAEWMklk4XylFlelZZN0f2Yuw3dz-Birzd1LF3TmNZ1Q9SS5AXNgSbw7g-47obQpr9pyohUHESRIBihMnQxBuf1NqSdwk4D0Xu3enSr9-XerVZp5vYQPNiNq44TB5kJoCMQt3uJLhxf_j_1F3UBgys</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>230795168</pqid></control><display><type>article</type><title>Tissue bioengineering for surgical bleb defects: an animal study</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Hsu, Wei-Cherng ; Ritch, Robert ; Krupin, Theodore ; Chen, Henry Shen-Lih</creator><creatorcontrib>Hsu, Wei-Cherng ; Ritch, Robert ; Krupin, Theodore ; Chen, Henry Shen-Lih</creatorcontrib><description>Background
To assess the effectiveness of OloGen (also named iGen), a porous, bioengineered, biodegradable, collagen-glycoaminoglycan matrix implant, in preventing poor bleb formation and early failure after trabeculectomy in eyes with a surgical wound defect.
Methods
The right eyes of 30 female New Zealand albino rabbits underwent trabeculectomy with OloGen implanted subconjunctivally on top of the scleral flap, while six right eyes received trabeculectomy without the implant to serve as a control group. A 1–2 mm diameter circular conjunctival defect was created in all eyes. Six rabbits in the group receiving the implant were sacrificed on days 3, 5, 7, 21, and 28. Rabbits in the control group were sacrificed on day 28. Perkins applanation tonometry, Seidel test and measurement of both the extent of the conjunctival defect and the anterior chamber depth were performed. Enucleated eyes were fixed in 4% formaldehyde and stained with hematoxylin and eosin (H&E) for general histological observation, and with Sirius and Fast-green stains to assess collagen deposition and cell migration.
Results
Seidel tests were negative for all operated and control eyes. No flat anterior chamber occurred in either group. With the exception of days 5 and 7, post-operative mean IOP difference is significant in both groups, (
P
> 0.05 for day 5, 7 and
P
< 0.05 for day 3, 14, 21 and 28). In the implant group, the mean IOP was reduced by between 42% and 35% at days 14, 21, and 28, whereas the mean IOP in the control group was reduced by between only 12% and 2%. In the implant group, histology showed randomized collagen deposition and microcyst formation in the bleb after the matrix had degraded completely at day 28. In the control group, histology showed dense collagen deposition subconjunctivally at day 28.
Conclusions
OloGen successfully serves as a 3-dimensional scaffold for cell migration and proliferation, and can prevent failure by maintaining the size of the bleb in the presence of a large wound defect. It might also be successful at repairing postoperative bleb leaks.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-007-0744-9</identifier><identifier>PMID: 18265997</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Absorbable Implants ; Animals ; Basic Science ; Biocompatible Materials ; Bioengineering ; Collagen ; Conjunctiva - surgery ; Female ; Glycosaminoglycans ; Intraocular Pressure ; Medicine ; Medicine & Public Health ; Ophthalmology ; Postoperative Complications - prevention & control ; Rabbits ; Sclera - pathology ; Sclera - surgery ; Surgical Flaps ; Tissue Engineering ; Trabeculectomy ; Wound Healing</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2008-05, Vol.246 (5), p.709-717</ispartof><rights>Springer-Verlag 2007</rights><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-75121a2fdb6f7fd79a95fa63083e63896971fb04bc7b1a61b7573789f23b5dcb3</citedby><cites>FETCH-LOGICAL-c369t-75121a2fdb6f7fd79a95fa63083e63896971fb04bc7b1a61b7573789f23b5dcb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00417-007-0744-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00417-007-0744-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18265997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hsu, Wei-Cherng</creatorcontrib><creatorcontrib>Ritch, Robert</creatorcontrib><creatorcontrib>Krupin, Theodore</creatorcontrib><creatorcontrib>Chen, Henry Shen-Lih</creatorcontrib><title>Tissue bioengineering for surgical bleb defects: an animal study</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Background
To assess the effectiveness of OloGen (also named iGen), a porous, bioengineered, biodegradable, collagen-glycoaminoglycan matrix implant, in preventing poor bleb formation and early failure after trabeculectomy in eyes with a surgical wound defect.
Methods
The right eyes of 30 female New Zealand albino rabbits underwent trabeculectomy with OloGen implanted subconjunctivally on top of the scleral flap, while six right eyes received trabeculectomy without the implant to serve as a control group. A 1–2 mm diameter circular conjunctival defect was created in all eyes. Six rabbits in the group receiving the implant were sacrificed on days 3, 5, 7, 21, and 28. Rabbits in the control group were sacrificed on day 28. Perkins applanation tonometry, Seidel test and measurement of both the extent of the conjunctival defect and the anterior chamber depth were performed. Enucleated eyes were fixed in 4% formaldehyde and stained with hematoxylin and eosin (H&E) for general histological observation, and with Sirius and Fast-green stains to assess collagen deposition and cell migration.
Results
Seidel tests were negative for all operated and control eyes. No flat anterior chamber occurred in either group. With the exception of days 5 and 7, post-operative mean IOP difference is significant in both groups, (
P
> 0.05 for day 5, 7 and
P
< 0.05 for day 3, 14, 21 and 28). In the implant group, the mean IOP was reduced by between 42% and 35% at days 14, 21, and 28, whereas the mean IOP in the control group was reduced by between only 12% and 2%. In the implant group, histology showed randomized collagen deposition and microcyst formation in the bleb after the matrix had degraded completely at day 28. In the control group, histology showed dense collagen deposition subconjunctivally at day 28.
Conclusions
OloGen successfully serves as a 3-dimensional scaffold for cell migration and proliferation, and can prevent failure by maintaining the size of the bleb in the presence of a large wound defect. It might also be successful at repairing postoperative bleb leaks.</description><subject>Absorbable Implants</subject><subject>Animals</subject><subject>Basic Science</subject><subject>Biocompatible Materials</subject><subject>Bioengineering</subject><subject>Collagen</subject><subject>Conjunctiva - surgery</subject><subject>Female</subject><subject>Glycosaminoglycans</subject><subject>Intraocular Pressure</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Ophthalmology</subject><subject>Postoperative Complications - prevention & control</subject><subject>Rabbits</subject><subject>Sclera - pathology</subject><subject>Sclera - surgery</subject><subject>Surgical Flaps</subject><subject>Tissue Engineering</subject><subject>Trabeculectomy</subject><subject>Wound Healing</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kM9LwzAUx4MoOqd_gBcpHrxF85ImaTwpw18w8DJht5C0yejo2pm0h_33ZnQwEIRAHo_P-ybvg9ANkAcgRD5GQnKQOJWYyDzH6gRNIGccS0KXp2hCJAVcMLq8QJcxrknCGYdzdAEFFVwpOUHPizrGwWW27ly7qlvnQt2uMt-FLA5hVZemyWzjbFY578o-PmWmTafepH7sh2p3hc68aaK7PtxT9P32uph94PnX--fsZY5LJlSPJQcKhvrKCi99JZVR3BvBSMGcYIUSSoK3JLeltGAEWMklk4XylFlelZZN0f2Yuw3dz-Birzd1LF3TmNZ1Q9SS5AXNgSbw7g-47obQpr9pyohUHESRIBihMnQxBuf1NqSdwk4D0Xu3enSr9-XerVZp5vYQPNiNq44TB5kJoCMQt3uJLhxf_j_1F3UBgys</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Hsu, Wei-Cherng</creator><creator>Ritch, Robert</creator><creator>Krupin, Theodore</creator><creator>Chen, Henry Shen-Lih</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>Tissue bioengineering for surgical bleb defects: an animal study</title><author>Hsu, Wei-Cherng ; Ritch, Robert ; Krupin, Theodore ; Chen, Henry Shen-Lih</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-75121a2fdb6f7fd79a95fa63083e63896971fb04bc7b1a61b7573789f23b5dcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Absorbable Implants</topic><topic>Animals</topic><topic>Basic Science</topic><topic>Biocompatible Materials</topic><topic>Bioengineering</topic><topic>Collagen</topic><topic>Conjunctiva - surgery</topic><topic>Female</topic><topic>Glycosaminoglycans</topic><topic>Intraocular Pressure</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Ophthalmology</topic><topic>Postoperative Complications - prevention & control</topic><topic>Rabbits</topic><topic>Sclera - pathology</topic><topic>Sclera - surgery</topic><topic>Surgical Flaps</topic><topic>Tissue Engineering</topic><topic>Trabeculectomy</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hsu, Wei-Cherng</creatorcontrib><creatorcontrib>Ritch, Robert</creatorcontrib><creatorcontrib>Krupin, Theodore</creatorcontrib><creatorcontrib>Chen, Henry Shen-Lih</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hsu, Wei-Cherng</au><au>Ritch, Robert</au><au>Krupin, Theodore</au><au>Chen, Henry Shen-Lih</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue bioengineering for surgical bleb defects: an animal study</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><stitle>Graefes Arch Clin Exp Ophthalmol</stitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>246</volume><issue>5</issue><spage>709</spage><epage>717</epage><pages>709-717</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><abstract>Background
To assess the effectiveness of OloGen (also named iGen), a porous, bioengineered, biodegradable, collagen-glycoaminoglycan matrix implant, in preventing poor bleb formation and early failure after trabeculectomy in eyes with a surgical wound defect.
Methods
The right eyes of 30 female New Zealand albino rabbits underwent trabeculectomy with OloGen implanted subconjunctivally on top of the scleral flap, while six right eyes received trabeculectomy without the implant to serve as a control group. A 1–2 mm diameter circular conjunctival defect was created in all eyes. Six rabbits in the group receiving the implant were sacrificed on days 3, 5, 7, 21, and 28. Rabbits in the control group were sacrificed on day 28. Perkins applanation tonometry, Seidel test and measurement of both the extent of the conjunctival defect and the anterior chamber depth were performed. Enucleated eyes were fixed in 4% formaldehyde and stained with hematoxylin and eosin (H&E) for general histological observation, and with Sirius and Fast-green stains to assess collagen deposition and cell migration.
Results
Seidel tests were negative for all operated and control eyes. No flat anterior chamber occurred in either group. With the exception of days 5 and 7, post-operative mean IOP difference is significant in both groups, (
P
> 0.05 for day 5, 7 and
P
< 0.05 for day 3, 14, 21 and 28). In the implant group, the mean IOP was reduced by between 42% and 35% at days 14, 21, and 28, whereas the mean IOP in the control group was reduced by between only 12% and 2%. In the implant group, histology showed randomized collagen deposition and microcyst formation in the bleb after the matrix had degraded completely at day 28. In the control group, histology showed dense collagen deposition subconjunctivally at day 28.
Conclusions
OloGen successfully serves as a 3-dimensional scaffold for cell migration and proliferation, and can prevent failure by maintaining the size of the bleb in the presence of a large wound defect. It might also be successful at repairing postoperative bleb leaks.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18265997</pmid><doi>10.1007/s00417-007-0744-9</doi><tpages>9</tpages></addata></record> |
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| identifier | ISSN: 0721-832X |
| ispartof | Graefe's archive for clinical and experimental ophthalmology, 2008-05, Vol.246 (5), p.709-717 |
| issn | 0721-832X 1435-702X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Absorbable Implants Animals Basic Science Biocompatible Materials Bioengineering Collagen Conjunctiva - surgery Female Glycosaminoglycans Intraocular Pressure Medicine Medicine & Public Health Ophthalmology Postoperative Complications - prevention & control Rabbits Sclera - pathology Sclera - surgery Surgical Flaps Tissue Engineering Trabeculectomy Wound Healing |
| title | Tissue bioengineering for surgical bleb defects: an animal study |
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