First confirmation of Bordetella pertussis occurrence in slovakia by using real-time PCR

By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis-pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS48...

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Veröffentlicht in:Central European journal of public health 2007-03, Vol.15 (1), p.38-43
Hauptverfasser: MAD'AROVA, Lucia, KLEMENT, Cyril, KOHUTOVA, Desana, TINAKOVA, Katarina, KRAJCIKOVA, Ludmila, OBERNAUEROVA, Margita
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container_end_page 43
container_issue 1
container_start_page 38
container_title Central European journal of public health
container_volume 15
creator MAD'AROVA, Lucia
KLEMENT, Cyril
KOHUTOVA, Desana
TINAKOVA, Katarina
KRAJCIKOVA, Ludmila
OBERNAUEROVA, Margita
description By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis-pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections.
doi_str_mv 10.21101/cejph.a3401
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subjects Adolescent
Bacterial diseases
Biological and medical sciences
Bordetella pertussis - genetics
Bordetella pertussis - isolation & purification
Child, Preschool
Ent and stomatologic bacterial diseases
Female
Human bacterial diseases
Humans
Infectious diseases
Male
Medical sciences
Miscellaneous
Polymerase Chain Reaction
Public health. Hygiene
Public health. Hygiene-occupational medicine
Slovakia
Whooping Cough - diagnosis
title First confirmation of Bordetella pertussis occurrence in slovakia by using real-time PCR
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