First confirmation of Bordetella pertussis occurrence in slovakia by using real-time PCR
By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis-pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS48...
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Veröffentlicht in: | Central European journal of public health 2007-03, Vol.15 (1), p.38-43 |
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container_title | Central European journal of public health |
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creator | MAD'AROVA, Lucia KLEMENT, Cyril KOHUTOVA, Desana TINAKOVA, Katarina KRAJCIKOVA, Ludmila OBERNAUEROVA, Margita |
description | By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis-pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections. |
doi_str_mv | 10.21101/cejph.a3401 |
format | Article |
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Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections.</description><identifier>ISSN: 1210-7778</identifier><identifier>DOI: 10.21101/cejph.a3401</identifier><identifier>PMID: 17491558</identifier><language>eng</language><publisher>Prague: Czech Medical Association J. E. 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Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections.</description><subject>Adolescent</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>Bordetella pertussis - genetics</subject><subject>Bordetella pertussis - isolation & purification</subject><subject>Child, Preschool</subject><subject>Ent and stomatologic bacterial diseases</subject><subject>Female</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>Polymerase Chain Reaction</subject><subject>Public health. Hygiene</subject><subject>Public health. 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Hygiene</topic><topic>Public health. Hygiene-occupational medicine</topic><topic>Slovakia</topic><topic>Whooping Cough - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAD'AROVA, Lucia</creatorcontrib><creatorcontrib>KLEMENT, Cyril</creatorcontrib><creatorcontrib>KOHUTOVA, Desana</creatorcontrib><creatorcontrib>TINAKOVA, Katarina</creatorcontrib><creatorcontrib>KRAJCIKOVA, Ludmila</creatorcontrib><creatorcontrib>OBERNAUEROVA, Margita</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Central European journal of public health</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MAD'AROVA, Lucia</au><au>KLEMENT, Cyril</au><au>KOHUTOVA, Desana</au><au>TINAKOVA, Katarina</au><au>KRAJCIKOVA, Ludmila</au><au>OBERNAUEROVA, Margita</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>First confirmation of Bordetella pertussis occurrence in slovakia by using real-time PCR</atitle><jtitle>Central European journal of public health</jtitle><addtitle>Cent Eur J Public Health</addtitle><date>2007-03</date><risdate>2007</risdate><volume>15</volume><issue>1</issue><spage>38</spage><epage>43</epage><pages>38-43</pages><issn>1210-7778</issn><abstract>By application of the real-time PCR we manage to confirm the diagnosis and occurrence of a disease, which is caused by Bordetella pertussis-pertussis. Using this method we have proven the presence of DNA of Bordetella pertussis in the biological materials (nasopharyngeal swabs). The presence of IS481 genome sequence of Bordetella pertussis was confirmed. This method of detection of pathogens seems to be very rapid, simple, and specific. In the case of adequate technical laboratory equipment it may become very suitable and important supporter in explanation and confirmation of the occurrence of bacterial infections.</abstract><cop>Prague</cop><pub>Czech Medical Association J. E. Purkyně</pub><pmid>17491558</pmid><doi>10.21101/cejph.a3401</doi><tpages>6</tpages></addata></record> |
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subjects | Adolescent Bacterial diseases Biological and medical sciences Bordetella pertussis - genetics Bordetella pertussis - isolation & purification Child, Preschool Ent and stomatologic bacterial diseases Female Human bacterial diseases Humans Infectious diseases Male Medical sciences Miscellaneous Polymerase Chain Reaction Public health. Hygiene Public health. Hygiene-occupational medicine Slovakia Whooping Cough - diagnosis |
title | First confirmation of Bordetella pertussis occurrence in slovakia by using real-time PCR |
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