MULTIPLEX PCR ASSAY FOR THE DETECTION OF ANOPHELES FLUVIATILIS SPECIES COMPLEX, HUMAN HOST PREFERENCE, AND PLASMODIUM FALCIPARUM SPOROZOITE PRESENCE, USING A UNIQUE MOSQUITO PROCESSING METHOD

A multiplex PCR assay has been developed for detection of Anopheles fluviatilis cryptic species, their human host preference, and Plasmodium falciparum presence in the mosquito. PCR conditions were optimized using primer sets specific for A. fluviatilis cryptic species, Homo sapiens, and P. falcipar...

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Veröffentlicht in:	The American journal of tropical medicine and hygiene 2007-05, Vol.76 (5), p.837-843
Hauptverfasser:	MOHANTY, AMITAV, KAR, PRIYANKA, MISHRA, KIRTI, SINGH, DURG V, MOHAPATRA, NAMITA, KAR, SANTANU K, DASH, ADITYA P, HAZRA, RUPENANGSHU K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anopheles - classification Anopheles - genetics Anopheles - parasitology Anopheles fluviatilis Biological and medical sciences DNA Primers - chemistry DNA, Ribosomal - genetics Host-Parasite Interactions Humans Infectious diseases Insect Vectors - classification Insect Vectors - genetics Insect Vectors - parasitology Malaria, Falciparum - transmission Medical sciences Plasmodium falciparum Plasmodium falciparum - genetics Plasmodium falciparum - isolation & purification Polymerase Chain Reaction - methods Sporozoites
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