Detection of bovine viral diarrhea virus (BVDV) in single or small groups of preimplantation bovine embryos

The objectives of this study were to develop techniques to detect BVDV associated with single or small groups of bovine embryos contained in small aliquots of medium using either virus isolation (VI) or real time quantitative polymerase chain reaction (RT-QPCR) assays. In vivo-derived and in vitro-p...

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Veröffentlicht in:	Theriogenology 2007-06, Vol.67 (9), p.1415-1423
Hauptverfasser:	Gard, J.A., Givens, M.D., Riddell, K.P., Galik, P.K., Zhang, Y., Stringfellow, D.A., Marley, M.S.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Blastocyst - virology Bovine viral diarrhea virus Bovine viral diarrhea virus (BVDV) Bovine Virus Diarrhea-Mucosal Disease - prevention & control Bovine Virus Diarrhea-Mucosal Disease - transmission Cattle Cattle - embryology Cattle - virology Culture Techniques Diarrhea Viruses, Bovine Viral - isolation & purification disease detection embryo (animal) embryo culture Female Fertilization in Vitro In vitro-produced embryos In vivo-derived embryos Infectious Disease Transmission, Vertical - veterinary isolation polymerase chain reaction Quantitative polymerase chain reaction (QPCR) real time quantitative polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - veterinary Sensitivity and Specificity virus transmission
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