Preliminary results of fetal Rhc examination in plasma of pregnant women with anti-c
Objectives Anti‐Rhc antibodies may be the reason for the hemolytic disease of the newborn, therefore, noninvasive Rhc determination is important for pregnancy monitoring. For this purpose, we decided to introduce real‐time polymerase chain reaction (PCR) method. Methods Blood from 200 donors, plasma...
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| Veröffentlicht in: | Prenatal diagnosis 2008-04, Vol.28 (4), p.335-337 |
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| creator | Orzińska, A. Guz, K. Brojer, E. Żupańska, B. |
| description | Objectives
Anti‐Rhc antibodies may be the reason for the hemolytic disease of the newborn, therefore, noninvasive Rhc determination is important for pregnancy monitoring. For this purpose, we decided to introduce real‐time polymerase chain reaction (PCR) method.
Methods
Blood from 200 donors, plasma and whole‐blood from 11 Rhc‐negative mothers, as well as blood from fathers and newborns were examined. Rhc sensitivity and specificity were first determined by real‐time PCR using genomic DNA from donors. The same Rhc genotyping method was used for fetal Rhc detection in maternal plasma. To confirm the fetal Rhc‐negative result, plasma was tested with a panel of biallelic insertion/deletion polymorphisms for the presence of fetal DNA.
Results
The c allele assay showed full specificity. The mean Ct value for one copy of c allele diluted in C‐negative DNA was determined from extrapolating the correlation curve as 39.9. Full concordance was observed between the fetal Rhc genotypes from maternal plasma and the newborn phenotypes.
Conclusions
Preliminary results show that it is possible to examine fetal c allele of RHCE gene in the plasma of pregnant women with anti‐c by means of a noninvasive method. The diagnostic accuracy of the procedure, however, has yet to be confirmed in a larger group. Copyright © 2008 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/pd.1977 |
| format | Article |
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Anti‐Rhc antibodies may be the reason for the hemolytic disease of the newborn, therefore, noninvasive Rhc determination is important for pregnancy monitoring. For this purpose, we decided to introduce real‐time polymerase chain reaction (PCR) method.
Methods
Blood from 200 donors, plasma and whole‐blood from 11 Rhc‐negative mothers, as well as blood from fathers and newborns were examined. Rhc sensitivity and specificity were first determined by real‐time PCR using genomic DNA from donors. The same Rhc genotyping method was used for fetal Rhc detection in maternal plasma. To confirm the fetal Rhc‐negative result, plasma was tested with a panel of biallelic insertion/deletion polymorphisms for the presence of fetal DNA.
Results
The c allele assay showed full specificity. The mean Ct value for one copy of c allele diluted in C‐negative DNA was determined from extrapolating the correlation curve as 39.9. Full concordance was observed between the fetal Rhc genotypes from maternal plasma and the newborn phenotypes.
Conclusions
Preliminary results show that it is possible to examine fetal c allele of RHCE gene in the plasma of pregnant women with anti‐c by means of a noninvasive method. The diagnostic accuracy of the procedure, however, has yet to be confirmed in a larger group. Copyright © 2008 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0197-3851</identifier><identifier>EISSN: 1097-0223</identifier><identifier>DOI: 10.1002/pd.1977</identifier><identifier>PMID: 18382999</identifier><identifier>CODEN: PRDIDM</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Alleles ; Biological and medical sciences ; c allele of the RHCE gene ; cell-free fetal DNA ; Delivery. Postpartum. Lactation ; Feasibility Studies ; Female ; Fetus - metabolism ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; Genotype ; Gestational Age ; Gynecology. Andrology. Obstetrics ; Humans ; Infant, Newborn ; Isoantibodies - blood ; Male ; Maternal-Fetal Exchange - genetics ; Maternal-Fetal Exchange - physiology ; Medical sciences ; Molecular and cellular biology ; Mothers ; noninvasive genotyping ; Pregnancy ; Prenatal Diagnosis - methods ; Rh-Hr Blood-Group System - blood ; Rh-Hr Blood-Group System - genetics ; Rh-Hr Blood-Group System - metabolism</subject><ispartof>Prenatal diagnosis, 2008-04, Vol.28 (4), p.335-337</ispartof><rights>Copyright © 2008 John Wiley & Sons, Ltd.</rights><rights>2008 INIST-CNRS</rights><rights>Copyright (c) 2008 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3837-67867505f28314ab0396ad59d5223a6a3a42a8d8dc802f4895d7f026e6461b7d3</citedby><cites>FETCH-LOGICAL-c3837-67867505f28314ab0396ad59d5223a6a3a42a8d8dc802f4895d7f026e6461b7d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpd.1977$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpd.1977$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20218467$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18382999$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Orzińska, A.</creatorcontrib><creatorcontrib>Guz, K.</creatorcontrib><creatorcontrib>Brojer, E.</creatorcontrib><creatorcontrib>Żupańska, B.</creatorcontrib><title>Preliminary results of fetal Rhc examination in plasma of pregnant women with anti-c</title><title>Prenatal diagnosis</title><addtitle>Prenat. Diagn</addtitle><description>Objectives
Anti‐Rhc antibodies may be the reason for the hemolytic disease of the newborn, therefore, noninvasive Rhc determination is important for pregnancy monitoring. For this purpose, we decided to introduce real‐time polymerase chain reaction (PCR) method.
Methods
Blood from 200 donors, plasma and whole‐blood from 11 Rhc‐negative mothers, as well as blood from fathers and newborns were examined. Rhc sensitivity and specificity were first determined by real‐time PCR using genomic DNA from donors. The same Rhc genotyping method was used for fetal Rhc detection in maternal plasma. To confirm the fetal Rhc‐negative result, plasma was tested with a panel of biallelic insertion/deletion polymorphisms for the presence of fetal DNA.
Results
The c allele assay showed full specificity. The mean Ct value for one copy of c allele diluted in C‐negative DNA was determined from extrapolating the correlation curve as 39.9. Full concordance was observed between the fetal Rhc genotypes from maternal plasma and the newborn phenotypes.
Conclusions
Preliminary results show that it is possible to examine fetal c allele of RHCE gene in the plasma of pregnant women with anti‐c by means of a noninvasive method. The diagnostic accuracy of the procedure, however, has yet to be confirmed in a larger group. Copyright © 2008 John Wiley & Sons, Ltd.</description><subject>Alleles</subject><subject>Biological and medical sciences</subject><subject>c allele of the RHCE gene</subject><subject>cell-free fetal DNA</subject><subject>Delivery. Postpartum. Lactation</subject><subject>Feasibility Studies</subject><subject>Female</subject><subject>Fetus - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genotype</subject><subject>Gestational Age</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Isoantibodies - blood</subject><subject>Male</subject><subject>Maternal-Fetal Exchange - genetics</subject><subject>Maternal-Fetal Exchange - physiology</subject><subject>Medical sciences</subject><subject>Molecular and cellular biology</subject><subject>Mothers</subject><subject>noninvasive genotyping</subject><subject>Pregnancy</subject><subject>Prenatal Diagnosis - methods</subject><subject>Rh-Hr Blood-Group System - blood</subject><subject>Rh-Hr Blood-Group System - genetics</subject><subject>Rh-Hr Blood-Group System - metabolism</subject><issn>0197-3851</issn><issn>1097-0223</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10MtO3DAUBmALgWCgqG9QeUNZVKG-JLazRECnCNSORqB2Z52JneLiXGpnNMPb19FEsGLl26f_HB-EPlJyQQlhX3tzQUsp99CMklJmhDG-j2Yk3WVcFfQIHcf4N0HFSnmIjqjiaVeWM_SwCNa7xrUQXnCwce2HiLsa13YAj5dPFbZbGJ8H17XYtbj3EBsYSR_snxbaAW-6xrZ444YnnI4uqz6ggxp8tKfTeoIev908XH3P7n_Ob68u77OKKy4zIZWQBSlqpjjNYUV4KcAUpSlS-yCAQ85AGWUqRVidq7IwsiZMWJELupKGn6DPu9w-dP_WNg66cbGy3kNru3XUkuRCFTJP8HwHq9DFGGyt--Ca9GVNiR4HqHujxwEm-WmKXK8aa97cNLEEziYAsQJfB2grF18dI4yqXIxBX3Zu47x9ea-eXlxPZbOddnGw21cN4VmnLFnoXz_mWizF77tFvtBL_h9-1pPC</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Orzińska, A.</creator><creator>Guz, K.</creator><creator>Brojer, E.</creator><creator>Żupańska, B.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Preliminary results of fetal Rhc examination in plasma of pregnant women with anti-c</title><author>Orzińska, A. ; Guz, K. ; Brojer, E. ; Żupańska, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3837-67867505f28314ab0396ad59d5223a6a3a42a8d8dc802f4895d7f026e6461b7d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alleles</topic><topic>Biological and medical sciences</topic><topic>c allele of the RHCE gene</topic><topic>cell-free fetal DNA</topic><topic>Delivery. Postpartum. Lactation</topic><topic>Feasibility Studies</topic><topic>Female</topic><topic>Fetus - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genotype</topic><topic>Gestational Age</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Isoantibodies - blood</topic><topic>Male</topic><topic>Maternal-Fetal Exchange - genetics</topic><topic>Maternal-Fetal Exchange - physiology</topic><topic>Medical sciences</topic><topic>Molecular and cellular biology</topic><topic>Mothers</topic><topic>noninvasive genotyping</topic><topic>Pregnancy</topic><topic>Prenatal Diagnosis - methods</topic><topic>Rh-Hr Blood-Group System - blood</topic><topic>Rh-Hr Blood-Group System - genetics</topic><topic>Rh-Hr Blood-Group System - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Orzińska, A.</creatorcontrib><creatorcontrib>Guz, K.</creatorcontrib><creatorcontrib>Brojer, E.</creatorcontrib><creatorcontrib>Żupańska, B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Prenatal diagnosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Orzińska, A.</au><au>Guz, K.</au><au>Brojer, E.</au><au>Żupańska, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preliminary results of fetal Rhc examination in plasma of pregnant women with anti-c</atitle><jtitle>Prenatal diagnosis</jtitle><addtitle>Prenat. Diagn</addtitle><date>2008-04</date><risdate>2008</risdate><volume>28</volume><issue>4</issue><spage>335</spage><epage>337</epage><pages>335-337</pages><issn>0197-3851</issn><eissn>1097-0223</eissn><coden>PRDIDM</coden><abstract>Objectives
Anti‐Rhc antibodies may be the reason for the hemolytic disease of the newborn, therefore, noninvasive Rhc determination is important for pregnancy monitoring. For this purpose, we decided to introduce real‐time polymerase chain reaction (PCR) method.
Methods
Blood from 200 donors, plasma and whole‐blood from 11 Rhc‐negative mothers, as well as blood from fathers and newborns were examined. Rhc sensitivity and specificity were first determined by real‐time PCR using genomic DNA from donors. The same Rhc genotyping method was used for fetal Rhc detection in maternal plasma. To confirm the fetal Rhc‐negative result, plasma was tested with a panel of biallelic insertion/deletion polymorphisms for the presence of fetal DNA.
Results
The c allele assay showed full specificity. The mean Ct value for one copy of c allele diluted in C‐negative DNA was determined from extrapolating the correlation curve as 39.9. Full concordance was observed between the fetal Rhc genotypes from maternal plasma and the newborn phenotypes.
Conclusions
Preliminary results show that it is possible to examine fetal c allele of RHCE gene in the plasma of pregnant women with anti‐c by means of a noninvasive method. The diagnostic accuracy of the procedure, however, has yet to be confirmed in a larger group. Copyright © 2008 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>18382999</pmid><doi>10.1002/pd.1977</doi><tpages>3</tpages></addata></record> |
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| subjects | Alleles Biological and medical sciences c allele of the RHCE gene cell-free fetal DNA Delivery. Postpartum. Lactation Feasibility Studies Female Fetus - metabolism Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Genotype Gestational Age Gynecology. Andrology. Obstetrics Humans Infant, Newborn Isoantibodies - blood Male Maternal-Fetal Exchange - genetics Maternal-Fetal Exchange - physiology Medical sciences Molecular and cellular biology Mothers noninvasive genotyping Pregnancy Prenatal Diagnosis - methods Rh-Hr Blood-Group System - blood Rh-Hr Blood-Group System - genetics Rh-Hr Blood-Group System - metabolism |
| title | Preliminary results of fetal Rhc examination in plasma of pregnant women with anti-c |
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