Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein–protein interactions
Testis-specific poly(A) polymerase (TPAP) is a cytoplasmic poly(A) polymerase that is highly expressed in round spermatids. We identified germ cell-specific gene 1 (GSG1) as a TPAP interaction partner protein using yeast two-hybrid and coimmunoprecipitation assays. Subcellular fractionation analysis...
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| Veröffentlicht in: | FEBS letters 2008-04, Vol.582 (8), p.1203-1209 |
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| creator | Choi, Hyun-Sook Lee, Seol-Hoon Kim, Hana Lee, Younghoon |
| description | Testis-specific poly(A) polymerase (TPAP) is a cytoplasmic poly(A) polymerase that is highly expressed in round spermatids. We identified germ cell-specific gene 1 (GSG1) as a TPAP interaction partner protein using yeast two-hybrid and coimmunoprecipitation assays. Subcellular fractionation analysis showed that GSG1 is exclusively localized in the endoplasmic reticulum (ER) of mouse testis where TPAP is also present. In NIH3T3 cells cotransfected with TPAP and GSG1, both proteins colocalize in the ER. Moreover, expression of GSG1 stimulates TPAP targeting to the ER, suggesting that interactions between the two proteins lead to the redistribution of TPAP from the cytosol to the ER.
MINT-6168263:Gsg1 (uniprotkb:Q8R1W2), TPAP (uniprotkb:Q9WVP6) and Calmegin (uniprotkb:P52194) colocalize (MI:0403) by cosedimentation (MI:0027)MINT-6168204, MINT-6168178:Gsg1 (uniprotkb:Q8R1W2) and TPAP (uniprotkb:Q9WVP6) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6167930:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by two-hybrid (MI:0018)MINT-6168112, MINT-6168011, MINT-6168054:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by coimmunoprecipitation (MI:0019)MINT-61668069, MINT-6168101:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by pull-down (MI:0096)MINT-6168218:Gsg1 (uniprotkb:Q8R1W2) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6168381:TPAP (uniprotkb:Q9WVP6) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416) |
| doi_str_mv | 10.1016/j.febslet.2008.01.065 |
| format | Article |
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MINT-6168263:Gsg1 (uniprotkb:Q8R1W2), TPAP (uniprotkb:Q9WVP6) and Calmegin (uniprotkb:P52194) colocalize (MI:0403) by cosedimentation (MI:0027)MINT-6168204, MINT-6168178:Gsg1 (uniprotkb:Q8R1W2) and TPAP (uniprotkb:Q9WVP6) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6167930:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by two-hybrid (MI:0018)MINT-6168112, MINT-6168011, MINT-6168054:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by coimmunoprecipitation (MI:0019)MINT-61668069, MINT-6168101:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by pull-down (MI:0096)MINT-6168218:Gsg1 (uniprotkb:Q8R1W2) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6168381:TPAP (uniprotkb:Q9WVP6) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416)</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2008.01.065</identifier><identifier>PMID: 18325338</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Adult ; Animals ; Cytoplasmic polyadenylation ; endoplasmic reticulum ; Endoplasmic Reticulum - enzymology ; germ cell-specific gene 1 protein ; GSG1 ; Humans ; Immunohistochemistry ; Immunoprecipitation ; Male ; Membrane protein ; Mice ; Microscopy, Confocal ; NIH 3T3 Cells ; Polynucleotide Adenylyltransferase - metabolism ; Protein Binding ; Protein-Serine-Threonine Kinases - metabolism ; Spermatogenesis ; Testis - enzymology ; testis-specific poly(A) polymerase ; TPAP</subject><ispartof>FEBS letters, 2008-04, Vol.582 (8), p.1203-1209</ispartof><rights>2008 Federation of European Biochemical Societies</rights><rights>FEBS Letters 582 (2008) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6031-c02207c83894a612fecf7ad150ca8336b35595e80b16bb68cb75f81624f434f23</citedby><cites>FETCH-LOGICAL-c6031-c02207c83894a612fecf7ad150ca8336b35595e80b16bb68cb75f81624f434f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2008.01.065$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2008.01.065$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18325338$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, Hyun-Sook</creatorcontrib><creatorcontrib>Lee, Seol-Hoon</creatorcontrib><creatorcontrib>Kim, Hana</creatorcontrib><creatorcontrib>Lee, Younghoon</creatorcontrib><title>Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein–protein interactions</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Testis-specific poly(A) polymerase (TPAP) is a cytoplasmic poly(A) polymerase that is highly expressed in round spermatids. We identified germ cell-specific gene 1 (GSG1) as a TPAP interaction partner protein using yeast two-hybrid and coimmunoprecipitation assays. Subcellular fractionation analysis showed that GSG1 is exclusively localized in the endoplasmic reticulum (ER) of mouse testis where TPAP is also present. In NIH3T3 cells cotransfected with TPAP and GSG1, both proteins colocalize in the ER. Moreover, expression of GSG1 stimulates TPAP targeting to the ER, suggesting that interactions between the two proteins lead to the redistribution of TPAP from the cytosol to the ER.
MINT-6168263:Gsg1 (uniprotkb:Q8R1W2), TPAP (uniprotkb:Q9WVP6) and Calmegin (uniprotkb:P52194) colocalize (MI:0403) by cosedimentation (MI:0027)MINT-6168204, MINT-6168178:Gsg1 (uniprotkb:Q8R1W2) and TPAP (uniprotkb:Q9WVP6) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6167930:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by two-hybrid (MI:0018)MINT-6168112, MINT-6168011, MINT-6168054:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by coimmunoprecipitation (MI:0019)MINT-61668069, MINT-6168101:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by pull-down (MI:0096)MINT-6168218:Gsg1 (uniprotkb:Q8R1W2) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6168381:TPAP (uniprotkb:Q9WVP6) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416)</description><subject>Adult</subject><subject>Animals</subject><subject>Cytoplasmic polyadenylation</subject><subject>endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - enzymology</subject><subject>germ cell-specific gene 1 protein</subject><subject>GSG1</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Immunoprecipitation</subject><subject>Male</subject><subject>Membrane protein</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>NIH 3T3 Cells</subject><subject>Polynucleotide Adenylyltransferase - metabolism</subject><subject>Protein Binding</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Spermatogenesis</subject><subject>Testis - enzymology</subject><subject>testis-specific poly(A) polymerase</subject><subject>TPAP</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkstu1TAQhi1ERQ-FRwB5hWCRdGwnjrNCpeoFqVIXhbWVOJNTH-WG7RSdHUv2vCFPgsOJ1GW7mrHmm98z-oeQdwxSBkye7tIWa99hSDmASoGlIPMXZMNUIRKRSfWSbABYluRFKY7Ja-93EN-Kla_IMVOC50KoDfl9ha6nBrsu8RMa21pDtzggZTRUbovB04A-WP9YnsZu__Hs0__Yo6s80jDScI8Uh2acusr3kXIYrJm7uY8VN87bezq5MaAd_v76s2bUDiH2m2DHwb8hR23VeXy7xhPy_fLi2_l1cnN79fX87CYxEgRLDHAOhVFClVklGW_RtEXVsBxMpYSQtcjzMkcFNZN1LZWpi7xVTPKszUTWcnFCPhx04xA_5ria7q1f9q8GHGevC8hkwXn5JMhBxRlk8SxQcCkimB9A40bvHbZ6crav3F4z0IupeqdXU_Viqgamo6mx7_36wVz32Dx2rS5G4PoA_LQd7p-nqi8vvvC75UKWAwEVswJYlPp8kMLowYNFp72xOBhsrEMTdDPaJ6b9B017zeQ</recordid><startdate>20080409</startdate><enddate>20080409</enddate><creator>Choi, Hyun-Sook</creator><creator>Lee, Seol-Hoon</creator><creator>Kim, Hana</creator><creator>Lee, Younghoon</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080409</creationdate><title>Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein–protein interactions</title><author>Choi, Hyun-Sook ; Lee, Seol-Hoon ; Kim, Hana ; Lee, Younghoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6031-c02207c83894a612fecf7ad150ca8336b35595e80b16bb68cb75f81624f434f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Cytoplasmic polyadenylation</topic><topic>endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - enzymology</topic><topic>germ cell-specific gene 1 protein</topic><topic>GSG1</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Immunoprecipitation</topic><topic>Male</topic><topic>Membrane protein</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>NIH 3T3 Cells</topic><topic>Polynucleotide Adenylyltransferase - metabolism</topic><topic>Protein Binding</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Spermatogenesis</topic><topic>Testis - enzymology</topic><topic>testis-specific poly(A) polymerase</topic><topic>TPAP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, Hyun-Sook</creatorcontrib><creatorcontrib>Lee, Seol-Hoon</creatorcontrib><creatorcontrib>Kim, Hana</creatorcontrib><creatorcontrib>Lee, Younghoon</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, Hyun-Sook</au><au>Lee, Seol-Hoon</au><au>Kim, Hana</au><au>Lee, Younghoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein–protein interactions</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2008-04-09</date><risdate>2008</risdate><volume>582</volume><issue>8</issue><spage>1203</spage><epage>1209</epage><pages>1203-1209</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Testis-specific poly(A) polymerase (TPAP) is a cytoplasmic poly(A) polymerase that is highly expressed in round spermatids. We identified germ cell-specific gene 1 (GSG1) as a TPAP interaction partner protein using yeast two-hybrid and coimmunoprecipitation assays. Subcellular fractionation analysis showed that GSG1 is exclusively localized in the endoplasmic reticulum (ER) of mouse testis where TPAP is also present. In NIH3T3 cells cotransfected with TPAP and GSG1, both proteins colocalize in the ER. Moreover, expression of GSG1 stimulates TPAP targeting to the ER, suggesting that interactions between the two proteins lead to the redistribution of TPAP from the cytosol to the ER.
MINT-6168263:Gsg1 (uniprotkb:Q8R1W2), TPAP (uniprotkb:Q9WVP6) and Calmegin (uniprotkb:P52194) colocalize (MI:0403) by cosedimentation (MI:0027)MINT-6168204, MINT-6168178:Gsg1 (uniprotkb:Q8R1W2) and TPAP (uniprotkb:Q9WVP6) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6167930:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by two-hybrid (MI:0018)MINT-6168112, MINT-6168011, MINT-6168054:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by coimmunoprecipitation (MI:0019)MINT-61668069, MINT-6168101:Gsg1 (uniprotkb:Q8R1W2) physically interacts (MI:0218) with TPAP (uniprotkb:Q9WVP6) by pull-down (MI:0096)MINT-6168218:Gsg1 (uniprotkb:Q8R1W2) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416)MINT-6168381:TPAP (uniprotkb:Q9WVP6) and GRP78 (uniprotkb:P20029) colocalize (MI:0403) by fluorescence microscopy (MI:0416)</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>18325338</pmid><doi>10.1016/j.febslet.2008.01.065</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Alma/SFX Local Collection |
| subjects | Adult Animals Cytoplasmic polyadenylation endoplasmic reticulum Endoplasmic Reticulum - enzymology germ cell-specific gene 1 protein GSG1 Humans Immunohistochemistry Immunoprecipitation Male Membrane protein Mice Microscopy, Confocal NIH 3T3 Cells Polynucleotide Adenylyltransferase - metabolism Protein Binding Protein-Serine-Threonine Kinases - metabolism Spermatogenesis Testis - enzymology testis-specific poly(A) polymerase TPAP |
| title | Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein–protein interactions |
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