Discrete microfluidics with electrochemical detection
A droplet-based electrochemical digital magnetofluidics system has been developed. The system relies on the magnetic movement, in air, of different aqueous microdroplets containing magnetic microparticles--serving as the 'sample', 'blank', 'wash' and 'reagent'...
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| Veröffentlicht in: | Analyst (London) 2007-05, Vol.132 (5), p.412-416 |
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| container_title | Analyst (London) |
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| creator | LINDSAY, Solitaire VAZQUEZ, Terannie EGATZ-GOMEZ, Ana LOYPRASERT, Suchera GARCIA, Antonio A WANG, Joseph |
| description | A droplet-based electrochemical digital magnetofluidics system has been developed. The system relies on the magnetic movement, in air, of different aqueous microdroplets containing magnetic microparticles--serving as the 'sample', 'blank', 'wash' and 'reagent' solutions--into and out of a three-electrode assembly. The movement of all droplets was controlled using the magnetic fields generated by three separate external magnets positioned below the superhydrophobic surface. Square-wave voltammetry was used for rapid measurements of dopamine in multiple successive microdrops with minimal cross talk. The ability of the droplet-based electrochemical microfluidic system to manipulate microliter solutions was also illustrated in bioassays of glucose, involving the merging of enzyme (GOx) and substrate droplets, followed by chronoamperometric measurements of the hydrogen peroxide product in the merged droplet. Variables of the new electrochemical digital magnetomicrofluidic technique were examined and optimized. The new droplet-based electrochemical microfluidic system offers a promising platform for automated clinical diagnostics and drug discovery. |
| doi_str_mv | 10.1039/b617631c |
| format | Article |
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The system relies on the magnetic movement, in air, of different aqueous microdroplets containing magnetic microparticles--serving as the 'sample', 'blank', 'wash' and 'reagent' solutions--into and out of a three-electrode assembly. The movement of all droplets was controlled using the magnetic fields generated by three separate external magnets positioned below the superhydrophobic surface. Square-wave voltammetry was used for rapid measurements of dopamine in multiple successive microdrops with minimal cross talk. The ability of the droplet-based electrochemical microfluidic system to manipulate microliter solutions was also illustrated in bioassays of glucose, involving the merging of enzyme (GOx) and substrate droplets, followed by chronoamperometric measurements of the hydrogen peroxide product in the merged droplet. Variables of the new electrochemical digital magnetomicrofluidic technique were examined and optimized. The new droplet-based electrochemical microfluidic system offers a promising platform for automated clinical diagnostics and drug discovery.</description><subject>Analytical chemistry</subject><subject>Applied sciences</subject><subject>Biological Assay - instrumentation</subject><subject>Biological Assay - methods</subject><subject>Chemistry</subject><subject>Dopamine - analysis</subject><subject>Electrochemical methods</subject><subject>Electrochemistry - instrumentation</subject><subject>Electrochemistry - methods</subject><subject>Exact sciences and technology</subject><subject>Global environmental pollution</subject><subject>Glucose - analysis</subject><subject>Magnetics</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidic Analytical Techniques - methods</subject><subject>Pollution</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1Lw0AQBuBFFFur4C-QXBQv0dnMfmSPWj-h4EXPId2dpStJU7Mp4r93pYEePQ3DPAwzL2PnHG44oLldKq4VcnvAphyVyKUsykM2BQDMCyXFhJ3E-JlaDhKO2YRroTmWasrkQ4i2p4GyNti-8802uGBj9h2GVUYN2aHv7IrSsG4yl5wdQrc-ZUe-biKdjXXGPp4e3-cv-eLt-XV-t8itAD7kEslZwQGM9p4jd5bIEwhjUeFSKQSsS1DKKSwKL7Sy0klDUjvDhSkRZ-xqt3fTd19bikPVpnOpaeo1ddtYaRASjFD_wsJoo5Q2CV7vYHo2xp58telDW_c_FYfqL8vqfpflPNGLced22ZLbwzG8BC5HUMcUj-_rtQ1x70otjTCIv6NPecY</recordid><startdate>200705</startdate><enddate>200705</enddate><creator>LINDSAY, Solitaire</creator><creator>VAZQUEZ, Terannie</creator><creator>EGATZ-GOMEZ, Ana</creator><creator>LOYPRASERT, Suchera</creator><creator>GARCIA, Antonio A</creator><creator>WANG, Joseph</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200705</creationdate><title>Discrete microfluidics with electrochemical detection</title><author>LINDSAY, Solitaire ; 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The system relies on the magnetic movement, in air, of different aqueous microdroplets containing magnetic microparticles--serving as the 'sample', 'blank', 'wash' and 'reagent' solutions--into and out of a three-electrode assembly. The movement of all droplets was controlled using the magnetic fields generated by three separate external magnets positioned below the superhydrophobic surface. Square-wave voltammetry was used for rapid measurements of dopamine in multiple successive microdrops with minimal cross talk. The ability of the droplet-based electrochemical microfluidic system to manipulate microliter solutions was also illustrated in bioassays of glucose, involving the merging of enzyme (GOx) and substrate droplets, followed by chronoamperometric measurements of the hydrogen peroxide product in the merged droplet. Variables of the new electrochemical digital magnetomicrofluidic technique were examined and optimized. The new droplet-based electrochemical microfluidic system offers a promising platform for automated clinical diagnostics and drug discovery.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><pmid>17471386</pmid><doi>10.1039/b617631c</doi><tpages>5</tpages></addata></record> |
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| ispartof | Analyst (London), 2007-05, Vol.132 (5), p.412-416 |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Analytical chemistry Applied sciences Biological Assay - instrumentation Biological Assay - methods Chemistry Dopamine - analysis Electrochemical methods Electrochemistry - instrumentation Electrochemistry - methods Exact sciences and technology Global environmental pollution Glucose - analysis Magnetics Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Pollution |
| title | Discrete microfluidics with electrochemical detection |
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