Detection of B7-H4 and p53 in pancreatic cancer: potential role as a cytological diagnostic adjunct
This study compared p53 expression with B7-H4, a novel cancer biomarker, in pancreatic ductal adenocarcinoma (PDA) resection specimens and in a pilot series of endoscopic ultrasound-guided fine-needle aspirations (EUS-FNAs). B7-H4 and p53 expression were evaluated by immunoperoxidase methods in 36 P...
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| Veröffentlicht in: | Pancreas 2008-03, Vol.36 (2), p.200-206 |
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| creator | Awadallah, Nida S Shroyer, Kenneth R Langer, Daniel A Torkko, Kathleen C Chen, Yang K Bentz, Joel S Papkoff, Jackie Liu, Wenhui Nash, S Russell Shah, Raj J |
| description | This study compared p53 expression with B7-H4, a novel cancer biomarker, in pancreatic ductal adenocarcinoma (PDA) resection specimens and in a pilot series of endoscopic ultrasound-guided fine-needle aspirations (EUS-FNAs).
B7-H4 and p53 expression were evaluated by immunoperoxidase methods in 36 PDA and 15 EUS-FNA specimens and were scored for intensity and proportion of positive cells; cases were then assigned a final sum score.
B7-H4 was detected in 33 (92%) of 36 PDA sections, 8 (89%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative EUS-FNAs. p53 was detected in 30 (83%) of 36 PDA sections, 4 (44%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative cases. One EUS-FNA case that was cytologically atypical but not diagnostic of malignancy expressed B7-H4 and p53. Some benign tissue components (intercalated cells/ducts, main pancreatic ducts, and acinar cells) were also positive for B7-H4 and/or p53. Overall expression of B7-H4 in benign tissues, however, was relatively low compared with that seen in most carcinoma cases.
B7-H4 was expressed more often in PDA than was p53. Despite potentially problematic expression in benign/normal cells, the 2 markers target different cellular components and demonstrate potential diagnostic use for detection of PDA in resected and EUS-FNA specimens. |
| doi_str_mv | 10.1097/MPA.0b013e318150e4e0 |
| format | Article |
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B7-H4 and p53 expression were evaluated by immunoperoxidase methods in 36 PDA and 15 EUS-FNA specimens and were scored for intensity and proportion of positive cells; cases were then assigned a final sum score.
B7-H4 was detected in 33 (92%) of 36 PDA sections, 8 (89%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative EUS-FNAs. p53 was detected in 30 (83%) of 36 PDA sections, 4 (44%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative cases. One EUS-FNA case that was cytologically atypical but not diagnostic of malignancy expressed B7-H4 and p53. Some benign tissue components (intercalated cells/ducts, main pancreatic ducts, and acinar cells) were also positive for B7-H4 and/or p53. Overall expression of B7-H4 in benign tissues, however, was relatively low compared with that seen in most carcinoma cases.
B7-H4 was expressed more often in PDA than was p53. Despite potentially problematic expression in benign/normal cells, the 2 markers target different cellular components and demonstrate potential diagnostic use for detection of PDA in resected and EUS-FNA specimens.</description><identifier>ISSN: 0885-3177</identifier><identifier>EISSN: 1536-4828</identifier><identifier>DOI: 10.1097/MPA.0b013e318150e4e0</identifier><identifier>PMID: 18376314</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Aged ; B7-1 Antigen - analysis ; Biomarkers, Tumor - analysis ; Biopsy, Fine-Needle - methods ; Carcinoma, Pancreatic Ductal - chemistry ; Carcinoma, Pancreatic Ductal - pathology ; Endosonography ; Female ; Humans ; Immunoenzyme Techniques ; Male ; Middle Aged ; Neoplasm Staging ; Pancreas - chemistry ; Pancreatic Neoplasms - chemistry ; Pancreatic Neoplasms - pathology ; Pilot Projects ; Predictive Value of Tests ; Reproducibility of Results ; Tissue Fixation ; Tumor Suppressor Protein p53 - analysis ; V-Set Domain-Containing T-Cell Activation Inhibitor 1</subject><ispartof>Pancreas, 2008-03, Vol.36 (2), p.200-206</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c254t-eddc30379ef5eed65f16fb9ef7ddc6a14f1960e6f4ebebbd2224b5de150add53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18376314$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Awadallah, Nida S</creatorcontrib><creatorcontrib>Shroyer, Kenneth R</creatorcontrib><creatorcontrib>Langer, Daniel A</creatorcontrib><creatorcontrib>Torkko, Kathleen C</creatorcontrib><creatorcontrib>Chen, Yang K</creatorcontrib><creatorcontrib>Bentz, Joel S</creatorcontrib><creatorcontrib>Papkoff, Jackie</creatorcontrib><creatorcontrib>Liu, Wenhui</creatorcontrib><creatorcontrib>Nash, S Russell</creatorcontrib><creatorcontrib>Shah, Raj J</creatorcontrib><title>Detection of B7-H4 and p53 in pancreatic cancer: potential role as a cytological diagnostic adjunct</title><title>Pancreas</title><addtitle>Pancreas</addtitle><description>This study compared p53 expression with B7-H4, a novel cancer biomarker, in pancreatic ductal adenocarcinoma (PDA) resection specimens and in a pilot series of endoscopic ultrasound-guided fine-needle aspirations (EUS-FNAs).
B7-H4 and p53 expression were evaluated by immunoperoxidase methods in 36 PDA and 15 EUS-FNA specimens and were scored for intensity and proportion of positive cells; cases were then assigned a final sum score.
B7-H4 was detected in 33 (92%) of 36 PDA sections, 8 (89%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative EUS-FNAs. p53 was detected in 30 (83%) of 36 PDA sections, 4 (44%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative cases. One EUS-FNA case that was cytologically atypical but not diagnostic of malignancy expressed B7-H4 and p53. Some benign tissue components (intercalated cells/ducts, main pancreatic ducts, and acinar cells) were also positive for B7-H4 and/or p53. Overall expression of B7-H4 in benign tissues, however, was relatively low compared with that seen in most carcinoma cases.
B7-H4 was expressed more often in PDA than was p53. Despite potentially problematic expression in benign/normal cells, the 2 markers target different cellular components and demonstrate potential diagnostic use for detection of PDA in resected and EUS-FNA specimens.</description><subject>Adult</subject><subject>Aged</subject><subject>B7-1 Antigen - analysis</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Biopsy, Fine-Needle - methods</subject><subject>Carcinoma, Pancreatic Ductal - chemistry</subject><subject>Carcinoma, Pancreatic Ductal - pathology</subject><subject>Endosonography</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Neoplasm Staging</subject><subject>Pancreas - chemistry</subject><subject>Pancreatic Neoplasms - chemistry</subject><subject>Pancreatic Neoplasms - pathology</subject><subject>Pilot Projects</subject><subject>Predictive Value of Tests</subject><subject>Reproducibility of Results</subject><subject>Tissue Fixation</subject><subject>Tumor Suppressor Protein p53 - analysis</subject><subject>V-Set Domain-Containing T-Cell Activation Inhibitor 1</subject><issn>0885-3177</issn><issn>1536-4828</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkDtPwzAUhS0EoqXwDxDyxJZix3acsJXyKFIRDN0jx76pXKVxsJ2h_x5XVEJiuq9zjnQ_hG4pmVNSyYePr8WcNIQyYLSkggAHcoamVLAi42VenqMpKUuRMSrlBF2FsCOESiaqSzShJZMFo3yK9DNE0NG6HrsWP8lsxbHqDR4Ew7bHg-q1BxWtxjq14B_x4CL00aoOe9cBVgErrA_RdW5rddoaq7a9C0eLMrux1_EaXbSqC3BzqjO0eX3ZLFfZ-vPtfblYZzoXPGZgjGaEyQpaAWAK0dKibdIk06FQlLe0KggULYcGmsbkec4bYSD9rowRbIbuf2MH775HCLHe26Ch61QPbgy1JJxXlOdJyH-F2rsQPLT14O1e-UNNSX1kWye29X-2yXZ3yh-bPZg_0wkm-wGksHc3</recordid><startdate>200803</startdate><enddate>200803</enddate><creator>Awadallah, Nida S</creator><creator>Shroyer, Kenneth R</creator><creator>Langer, Daniel A</creator><creator>Torkko, Kathleen C</creator><creator>Chen, Yang K</creator><creator>Bentz, Joel S</creator><creator>Papkoff, Jackie</creator><creator>Liu, Wenhui</creator><creator>Nash, S Russell</creator><creator>Shah, Raj J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200803</creationdate><title>Detection of B7-H4 and p53 in pancreatic cancer: potential role as a cytological diagnostic adjunct</title><author>Awadallah, Nida S ; Shroyer, Kenneth R ; Langer, Daniel A ; Torkko, Kathleen C ; Chen, Yang K ; Bentz, Joel S ; Papkoff, Jackie ; Liu, Wenhui ; Nash, S Russell ; Shah, Raj J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c254t-eddc30379ef5eed65f16fb9ef7ddc6a14f1960e6f4ebebbd2224b5de150add53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>B7-1 Antigen - analysis</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Biopsy, Fine-Needle - methods</topic><topic>Carcinoma, Pancreatic Ductal - chemistry</topic><topic>Carcinoma, Pancreatic Ductal - pathology</topic><topic>Endosonography</topic><topic>Female</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Neoplasm Staging</topic><topic>Pancreas - chemistry</topic><topic>Pancreatic Neoplasms - chemistry</topic><topic>Pancreatic Neoplasms - pathology</topic><topic>Pilot Projects</topic><topic>Predictive Value of Tests</topic><topic>Reproducibility of Results</topic><topic>Tissue Fixation</topic><topic>Tumor Suppressor Protein p53 - analysis</topic><topic>V-Set Domain-Containing T-Cell Activation Inhibitor 1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Awadallah, Nida S</creatorcontrib><creatorcontrib>Shroyer, Kenneth R</creatorcontrib><creatorcontrib>Langer, Daniel A</creatorcontrib><creatorcontrib>Torkko, Kathleen C</creatorcontrib><creatorcontrib>Chen, Yang K</creatorcontrib><creatorcontrib>Bentz, Joel S</creatorcontrib><creatorcontrib>Papkoff, Jackie</creatorcontrib><creatorcontrib>Liu, Wenhui</creatorcontrib><creatorcontrib>Nash, S Russell</creatorcontrib><creatorcontrib>Shah, Raj J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pancreas</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Awadallah, Nida S</au><au>Shroyer, Kenneth R</au><au>Langer, Daniel A</au><au>Torkko, Kathleen C</au><au>Chen, Yang K</au><au>Bentz, Joel S</au><au>Papkoff, Jackie</au><au>Liu, Wenhui</au><au>Nash, S Russell</au><au>Shah, Raj J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of B7-H4 and p53 in pancreatic cancer: potential role as a cytological diagnostic adjunct</atitle><jtitle>Pancreas</jtitle><addtitle>Pancreas</addtitle><date>2008-03</date><risdate>2008</risdate><volume>36</volume><issue>2</issue><spage>200</spage><epage>206</epage><pages>200-206</pages><issn>0885-3177</issn><eissn>1536-4828</eissn><abstract>This study compared p53 expression with B7-H4, a novel cancer biomarker, in pancreatic ductal adenocarcinoma (PDA) resection specimens and in a pilot series of endoscopic ultrasound-guided fine-needle aspirations (EUS-FNAs).
B7-H4 and p53 expression were evaluated by immunoperoxidase methods in 36 PDA and 15 EUS-FNA specimens and were scored for intensity and proportion of positive cells; cases were then assigned a final sum score.
B7-H4 was detected in 33 (92%) of 36 PDA sections, 8 (89%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative EUS-FNAs. p53 was detected in 30 (83%) of 36 PDA sections, 4 (44%) of 9 cytologically positive EUS-FNAs, and 1 (20%) of 5 cytologically negative cases. One EUS-FNA case that was cytologically atypical but not diagnostic of malignancy expressed B7-H4 and p53. Some benign tissue components (intercalated cells/ducts, main pancreatic ducts, and acinar cells) were also positive for B7-H4 and/or p53. Overall expression of B7-H4 in benign tissues, however, was relatively low compared with that seen in most carcinoma cases.
B7-H4 was expressed more often in PDA than was p53. Despite potentially problematic expression in benign/normal cells, the 2 markers target different cellular components and demonstrate potential diagnostic use for detection of PDA in resected and EUS-FNA specimens.</abstract><cop>United States</cop><pmid>18376314</pmid><doi>10.1097/MPA.0b013e318150e4e0</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged B7-1 Antigen - analysis Biomarkers, Tumor - analysis Biopsy, Fine-Needle - methods Carcinoma, Pancreatic Ductal - chemistry Carcinoma, Pancreatic Ductal - pathology Endosonography Female Humans Immunoenzyme Techniques Male Middle Aged Neoplasm Staging Pancreas - chemistry Pancreatic Neoplasms - chemistry Pancreatic Neoplasms - pathology Pilot Projects Predictive Value of Tests Reproducibility of Results Tissue Fixation Tumor Suppressor Protein p53 - analysis V-Set Domain-Containing T-Cell Activation Inhibitor 1 |
| title | Detection of B7-H4 and p53 in pancreatic cancer: potential role as a cytological diagnostic adjunct |
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