Quantitative 2-D gel electrophoresis-based expression proteomics of albumin and IgG immunodepleted plasma

Proteomic analysis of plasma is challenging because of its large dynamic range, which prevents the detection of low abundance proteins. Immunodepletion of high abundance proteins, such as albumin and IgG, has emerged as a favored technology to overcome this problem; however its suitability in quanti...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2008-04, Vol.865 (1), p.147-152
Hauptverfasser:	Seferovic, Maxim D., Krughkov, Violet, Pinto, Devanand, Han, Victor K.M., Gupta, Madhulika B.
Format:	Artikel
Sprache:	eng
Schlagworte:	2-DGE software Albumin Albumins - isolation & purification Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Electrophoresis, Gel, Two-Dimensional - methods ELISA Enzyme-Linked Immunosorbent Assay Expression proteomics Fundamental and applied biological sciences. Psychology General pharmacology Humans IgG Immunodepletion Immunoglobulin G - blood Immunoglobulin G - isolation & purification Medical sciences Pharmacology. Drug treatments Plasma proteome Proteomics Reproducibility of Results Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry
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