Characterization of recombinant foot-and-mouth disease virus pentamer-like structures expressed by baculovirus and their use as diagnostic antigens in a blocking ELISA

Abstract Non-infectious recombinant pentamer-like structures of the foot-and-mouth disease virus (FMDV) were expressed by baculovirus, and the antigenicity and immunogenicity of the proteins were analyzed in a blocking ELISA for the detection of FMDV antibodies. The recombinant pentamer-like structu...

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Veröffentlicht in:	Vaccine 2007-05, Vol.25 (20), p.4112-4121
Hauptverfasser:	Oem, Jae-Ku, Park, Jong-Hyeon, Lee, Kwang-Nyeong, Kim, Yong-Joo, Kye, Soo-Jeong, Park, Jee-Yong, Song, Hee-Jong
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergy and Immunology Animals Antigens Antigens, Viral - biosynthesis Antigens, Viral - genetics Antigens, Viral - immunology Applied microbiology Baculoviridae - genetics Baculoviridae - immunology Baculovirus Biological and medical sciences Blocking ELISA Cattle Centrifugation Cloning Enzyme-Linked Immunosorbent Assay - methods Enzymes Female Foot & mouth disease Foot-and-mouth disease virus Foot-and-Mouth Disease Virus - genetics Foot-and-Mouth Disease Virus - immunology Fundamental and applied biological sciences. Psychology Gene amplification Genomes Immunization Immunogenicity Infections Insects Laboratories Mice Mice, Inbred BALB C Microbiology Miscellaneous Pentameric subunits Plasmids Proteins Spodoptera - virology Swine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Virology Viruses
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